Skip to main content
Log in

UV-B radiation and cadmium induced changes in growth, photosynthesis, and antioxidant enzymes of cyanobacterium Plectonema boryanum

  • Published:
Biologia Plantarum

Abstract

UV-B (0.4 W m−2) irradiation and cadmium (2 and 8 μM) treatments separately inhibited the survival, growth, pigment content, and photosynthetic electron transport in Plectonema boryanum. Phycocyanin was the main target to UV-B and Cd and it was followed by chlorophyll a and carotenoids. UV-B and Cd caused strong inhibition on activities of photosystem 2 (PS2) and the whole electron transport chain, whereas photosystem 1 (PS1) was the least affected. UV-B and Cd treatment accelerated respiration, lipid peroxidation, and the activities of superoxide dismutase and catalase. However, enhancement in catalase activity was considerably less (5 − 50 %) as compared to SOD activity. As compared to individual treatment, the effect of their combination (UV-B + Cd) was more detrimental to the above parameters. A synergistic interaction of UV-B and Cd is probably due to increased cadmium uptake as a result of increased membrane permeability caused by lipid peroxidation in P. boryanum after UV-B exposure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

ASC:

ascorbate

Car:

carotenoids

Chl:

chlorophyll

DCMU:

3-(3′-4′-dichlorophenyl)-1,1-dimethyl urea

DCPIP:

2,6-dichlorophenol indophenol

MDA:

malondialdehyde

MV:

methyl viologen

pBQ:

p-benzoquinone

PC:

phycocyanin

PS:

photosystem

UV-B:

ultraviolet radiation (280–315 nm)

References

  • Adhikary, S.P.: Heat shock protein in the terrestrial epilithic cyanobacterium Tolypothrix byssoidea.-Biol. Plant. 47: 125–128, 2003/4.

    Article  CAS  Google Scholar 

  • Almog, O., Lotan, O., Shoham, G., Nechushtai, R.: The composition and organisation of photosystem I.-J. basic clin. Physiol. Pharmacol. 2: 123–140, 1991.

    PubMed  CAS  Google Scholar 

  • Bazzaz, M.B., Govindjee: Effects of cadmium nitrate on spectral characteristics and light reactions of chloroplasts.-Environ. Sci. Lett. 6: 1–12, 1974.

    Article  CAS  Google Scholar 

  • Bennett, A., Bogorad, L.: Complementary chromatic adaptation in a filamentous blue-green algae.-J. Cell Biol. 58: 419–435, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Caldwell, M.M.: Plant life and ultraviolet radiation: some perspectives in the history in the earth’s UV climate.-Bioscience 29: 520–525, 1979.

    Article  Google Scholar 

  • Caldwell, M.M.: Plant response to solar ultraviolet radiation.-In: Lange, O.L., Nobel, P.S., Osmond, O.S., Ziegler, H. (ed.): Physiological Plant Ecology. Vol. 12 A. Pp. 169–197. Springer-Verlag, Berlin 1981.

    Google Scholar 

  • Coohill, T.P.: Action spectra again?-Photochem. Photobiol. 54: 859–870, 1991.

    Article  PubMed  CAS  Google Scholar 

  • Dai, Q., Yan, B., Huang, S., Liu, X., Peng, S., Miranda, M.L.M., Chavez, A.Q., Vegara, B.S., Olszyk, D.: Response of oxidative stress defense systems in rice (Oryza sativa) leaves with supplemental UV-B radiation.-Physiol. Plant. 101: 301–308, 1997.

    Article  CAS  Google Scholar 

  • Döhler, G.: Effect of UV-B radiation (290–320 nm) on the nitrogen metabolism of several marine diatoms.-J. Plant Physiol. 118: 391–400, 1985.

    Google Scholar 

  • Dubey, R.S.: Photosynthesis in plants under stressful conditions.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 859–876. M. Dekker, New York 1997.

    Google Scholar 

  • Egashira, T., Takahama, U., Nakamura, K.: A reduced activity of catalase as a basis for light dependent methionine sensitivity of a Chlamydomonas reinhardtii mutant.-Plant Cell Physiol. 30: 1171–1175, 1989.

    CAS  Google Scholar 

  • Foyer, C.H., Delgado, H.L., Dat, J.F., Scott, I.M.: Hydrogen peroxide and glutathione associated mechanisms of acclamatory stress tolerance and signalling.-Physiol. Plant. 100: 241–254, 1997.

    Article  CAS  Google Scholar 

  • Frederick, J.E., Lubin, D.: Possible long-term changes in biologically active ultraviolet radiation reaching the ground.-Photochem. Photobiol. 47: 571–578, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Friso, G., Spetea, C., Giacometti, G.M., Vass, I., Barbato, R.: Degradation of photosystem II reaction center D1 protein induced by UV-B radiation in isolated thylakoids: Identification and characterization of C and N terminal break down products.-Biochim. biophys. Acta 1184: 78–84, 1994.

    Article  CAS  Google Scholar 

  • Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases: I. Occurrence in higher plants.-Plant Physiol. 59: 309–314, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Girotti, A.W.: Photodynamic lipid peroxidation in biological systems.-Photochem. Photobiol. 51: 497–509, 1990.

    Article  PubMed  CAS  Google Scholar 

  • Häder, D.P., Häder, M.A.: Effects of enhanced solar ultraviolet radiation on aquatic ecosystems.-Photochem. Photobiol. 53: 717–725, 1989.

    Article  Google Scholar 

  • Häder, D.P., Watanabe, M., Furuya, M.: Inhibition of motility in the cyanobacterium Phormidium uncinatum by solar and monochromatic UV-irradiation.-Plant Cell Physiol. 27: 887–894, 1986.

    Google Scholar 

  • Halliwell, B., Gutteridge, M.C.: Oxygen toxicity, oxygen radicals, transition metals and disease.-Biochem. J. 219: 1–14, 1984.

    PubMed  CAS  Google Scholar 

  • Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts, I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189–198, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Hideg, E., Vass, I.: UV-B induced free radical production in plant leaves and isolated thylakoid membranes.-Plant Sci. 115: 251–260, 1996.

    Article  CAS  Google Scholar 

  • Husaini, Y., Rai, L.C.: Studies on nitrogen and phosphorus metabolism and the photosynthetic electron transport system of Nostoc linckia under cadmium stress.-J. Plant Physiol. 138: 429–435, 1991.

    CAS  Google Scholar 

  • Jensen, A.: Chlorophylls and carotenoids.-In: Hellebust, J.A., Craige, J.S. (ed.): Handbook of Phycological Methods. Pp. 60–70. Cambridge University Press, Cambridge 1978.

    Google Scholar 

  • Karentz, D., Cleaver, J.E., Mitchell, D.L.: Cell survival characteristics and molecular responces of antarctic phytoplankton to ultraviolet-B radiation.-J. Phycol. 27: 326–341, 1991.

    Article  CAS  Google Scholar 

  • Kondo, N., Kawashima, M.: Enhancement of the tolerance to oxidative stress in cucumber (Cucumis sativus L.) seedlings by UV-B irradiation: Possible involvement of phenolic compounds and antioxidative enzymes.-J. Plant Res. 113: 311–317, 2000.

    Article  CAS  Google Scholar 

  • Lösch, R., Köhl, K.I.: Plant respiration under the influence of heavy metals.-In: Prasad, M.N.V., Hagemeyer, J.(ed.): Heavy Metal Stress in Plants from Molecules to Ecosystems. Pp. 139–156. Springer-Verlag, Berlin 1999.

    Google Scholar 

  • Mackinney, G.: Absorption of light by chlorophyll solutions.-J. biol. Chem. 140: 315–322, 1941.

    CAS  Google Scholar 

  • Mallick, N., Rai, L.C.: Studies on mineral uptake and enzyme activities of Anabaena doliolum under metal stress.-J. gen. appl. Microbiol. 40: 123–133, 1994.

    Article  CAS  Google Scholar 

  • Mallick, N., Rai, L.C.: Response of the antioxidant systems of the nitrogen fixing cyanobacterium Anabaena doliolum to copper.-J. Plant Physiol. 155: 146–149, 1999.

    CAS  Google Scholar 

  • Mathur, A., Sharma, Y.C., Rupainwar, D.C., Murthy, R.C., Chandra, S.: A study of river Ganga at Varanasi with special emphasis on heavy metal pollution.-Pollut. Res. 6 37–44, 1987.

    CAS  Google Scholar 

  • Melis, A., Nemson, J.A., Harrison, M.A.: Damage to functional components and partial degradation of photosystem II reaction centre proteins upon chloroplast exposure to ultraviolet-B radiation.-Biochim. biophys. Acta 1100: 312–320, 1992.

    CAS  Google Scholar 

  • Middleton, E.M., Teramura, A.H.: The role of flavonol glycosides and carotenoids in protecting soybean from ultraviolet-B damage.-Plant Physiol. 103: 741–752, 1993.

    PubMed  CAS  Google Scholar 

  • Mittler, R., Tel-Or, E.: Oxidative stress responses in the unicellular cyanobacterium Synechococcus PCC 7942.-Free Radical Res. Commun. 12: 845–850, 1991.

    Article  Google Scholar 

  • Moustakas, M., Lanaras, T., Symeonidis, L., Karataglis, S.: Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress.-Photosynthetica 30: 389–396, 1997.

    Google Scholar 

  • Nedunchezhian, N., Annamalainathan, K., Kulandaivelu, G.: Ultraviolet-B (280–320 nm) enhanced radiation induced changes in chlorophyll-protein complexes and polypeptide composition of chloroplasts in Vigna unguiculata seedlings grown at various temperatures.-Photosynthetica 31: 21–29, 1995.

    CAS  Google Scholar 

  • Newton, J.W., Tyler, D.D., Slod Ki, M.E.: Effect of ultraviolet-B (280–320 nm) radiation on blue green algae (cyanobacteria): Possible biological indicators of stratospheric ozone depletion.-Appl. environ. Microbiol. 37: 1134–1141, 1979.

    Google Scholar 

  • Okamoto, O.K., Asano, C.S., Aidar, E., Colepicolo, P.: Effects of cadmium on growth and superoxide dismutase activity of the marine microalga Tetraselmis gracilis (Prasinophyceae).-J. Phycol. 32: 74–79, 1996.

    Article  CAS  Google Scholar 

  • Prasad, M.N.V., Strzalka, K.: Impact of heavy metals on photosynthesis.-In: Prasad, M.N.V., Hagemeyer, J. (ed.): Heavy Metal Stress in Plants from Molecules to Ecosystems. Pp. 117–138. Springer-Verlag, Berlin 1999.

    Google Scholar 

  • Prasad, S.M., Singh, J.B., Rai, L.C., Kumar, H.D.: Metal induced inhibition of photosynthetic electron transport chain of the cyanobacterium N. muscorum.-FEMS Microbiol. Lett. 82: 95–100, 1991.

    Article  CAS  Google Scholar 

  • Quesada, A., Mouget, J.L., Vincent, W.F.: Growth of antarctic cyanobacteria under ultraviolet radiation; UV-A counteracts UV-B inhibition.-J. Phycol. 31: 242–248, 1995.

    Article  Google Scholar 

  • Rai, L.C., Raizada, M., Mallick, N., Husaini, Y., Singh, A.K., Dubey, S.K.: Effect of four heavy metals on the biology of Nostoc muscorum.-Biol. Metals 2: 229–234, 1990.

    Article  CAS  Google Scholar 

  • Rai, L.C., Tyagi, B., Mallick, N., Rai, P.K.: Interactive effect of UV-B and copper on photosynthetic activity of the cyanobacterium Anabaena doliolum.-Environ. exp. Bot. 35: 177–185, 1995.

    Article  CAS  Google Scholar 

  • Rai, L.C., Tyagi, B., Rai, P.K., Mallick, N.: Interactive effects of UV-B and heavy metals (Cu and Pb) on nitrogen and phosphorus metabolism of N2 fixing cyanobacterium Anabaena doliolum.-Environ. exp. Bot. 39: 221–231, 1998.

    Article  CAS  Google Scholar 

  • Sinha, R.P., Häder, D.P.: Effects of ultraviolet-B radiation in three rice field cyanobacteria.-J. Plant Physiol. 153: 763–769, 1998.

    CAS  Google Scholar 

  • Skórzyńska-Polit, E., Drążkiewiez, M., Krupa, Z.: The activity of the antioxidative system in cadmium-treated Arabidopsis thaliana.-Biol. Plant. 47: 71–78, 2003/4.

    Article  Google Scholar 

  • Spiller, H.: Photophosphorylation capacity of stable spheroplast preparations of Anabaena.-Plant Physiol. 66: 446–450, 1980.

    Article  PubMed  CAS  Google Scholar 

  • Stratton, G.W., Corke, C.T.: The effect of cadmium ion on the growth, photosynthesis and nitrogenase activity of Anabaena inaequalis.-Chemosphere 5: 277–282, 1979.

    Article  Google Scholar 

  • Tyagi, R., Srinivas, G., Vyas, D., Kumar, A., Kumar, H.D.: Differential effect of ultraviolet-B radiation on certain metabolic processes in a chromatically adapting Nostoc.-Photochem. Photobiol. 55: 401–407, 1992.

    Article  PubMed  CAS  Google Scholar 

  • Vincent, W.F., Roy, S.: Solar ultraviolet-B radiation and aquatic primary production damage, protection and recovery.-Environ. Rev. 1: 1–12, 1993.

    CAS  Google Scholar 

  • Wong, P.T.S., Burnison, G., Chau, Y.K.: Cadmium toxicity to freshwater algae.-Bull. environ. Contam. Toxicol. 23: 487–490, 1979.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. M. Prasad.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prasad, S.M., Zeeshan, M. UV-B radiation and cadmium induced changes in growth, photosynthesis, and antioxidant enzymes of cyanobacterium Plectonema boryanum . Biol Plant 49, 229–236 (2005). https://doi.org/10.1007/s10535-005-0236-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-005-0236-x

Additional key words

Navigation