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Somatic embryogenesis and plant regeneration in diploid and triploid Arachis pintoi

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Biologia Plantarum

Abstract

Plants of two cytotypes (2n=2x=20, and 2n=3x=30) of pinto peanut (Arachis pintoi Krapov. & W.C. Gregory) were regenerated through somatic embryogenesis. Embryogenic calli were induced from shoot tips or immature leaves dissected from in vitro growing plants. In the case of the diploid peanut the best somatic embryogenesis was achieved when shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with 10 mg dm−3 Picloram (PIC) and 0.1 mg dm−3 6-benzylaminopurine (BAP) or when explants from immature leaves were cultured on MS + 10 mg dm−3 PIC + 0.01 mg dm−3 BAP. In the case of triploid peanut the highest number of somatic embryos was obtained when shoot tips were cultured on MS + 10 mg dm−3 PIC + 0.01 mg dm−3 BAP or when immature leaves were cultured on MS + 20 mg dm−3 PIC + 0.01 mg dm−3 BAP. Somatic embryos were converted into plants by culture on MS + 0.01 mg dm−3 naphthaleneacetic acid + 0.01 mg dm−3 BAP. Plants were successfully transferred to pots in greenhouse.

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Abbreviations

BAP:

6-benzylaminopurine

MS medium:

Murashige and Skoog (1962) medium + 3 % sucrose + 0.65 % agar

NAA:

1-naphthaleneacetic acid

PIC:

picloram (4-amino-3,5,6-trichloropicolinic acid)

REYI:

relative embryogenic yield index = (% of calli with SE) × (number of SE per callus)

SE:

somatic embryos

References

  • Ammirato, P.V.: Embryogenesis.-In: Evans, E.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Techniques for Propagation and Breeding. Pp. 82–123. Macmillan Publ. Co., London 1983.

    Google Scholar 

  • Baker, C.M., Wetzstein, H.Y.: Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea.-Plant Cell Rep. 11: 71–75, 1992.

    Article  CAS  Google Scholar 

  • Baker, C.M., Wetzstein, H.Y.: Leaflet development, induction time, and medium influence somatic embryogenesis in peanut (Arachis hypogaea L.).-Plant Cell Rep. 17: 925–929, 1998.

    Article  CAS  Google Scholar 

  • Chengalrayan, K., Sathaye, S.S., Hazra, S.: Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis hypogaea L.).-Plant Cell Rep. 13: 578–581, 1994.

    Article  CAS  Google Scholar 

  • Durham, R.E., Parrott, W.A.: Repetitive somatic embryogenesis from peanut cultures in liquid medium.-Plant Cell Rep. 11: 122–125, 1992.

    Article  Google Scholar 

  • Eapen, S., George, L., Rao, P.S.: Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.).-Biol. Plant. 35: 499–504. 1993.

    Article  Google Scholar 

  • Fernandez, A., Krapovickas, A.: Cromosomas y evolucion en Arachis (Leguminosae).-Bonplandia 8: 187–220, 1994.

    Google Scholar 

  • Halamkova, E., Vagera, J., Ohnoutkova, L.: Regeneration capacity of calli derived from immature embryos in spring barley cultivars.-Biol. Plant. 48: 313–316, 2004.

    Google Scholar 

  • Joshi, M.V., Sahasrabudhe, N.A., Hazra, S.: Responses of peanut somatic embryos to thidiazuron.-Biol. Plant. 46: 187–192, 2003.

    Article  CAS  Google Scholar 

  • Krapovickas, A., Gregory, W.C.: Taxonomia del genero Arachis (Leguminosae).-Bonplandia 8: 1–186, 1994.

    Google Scholar 

  • Martin, K.P.: Benzyladenine induced somatic embryogenesis and plant regeneration of Leptadenia reticulata.-Biol. Plant. 48: 285–288, 2004.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    CAS  Google Scholar 

  • Penaloza, A.P., Pozzobon, M.T., Valls, J.F.M.: Cytogenetic findings in wild species of Arachis (Leguminosae).-Rev. Brasil. Genet. 19: 129, 1996.

    Google Scholar 

  • Pizarro, E.A., Rincon, A.: Regional experience with forage Arachis in South America.-In: Kerridge, P.C., Hardy, W. (ed.): Biology and Agronomy of Forage Arachis. Pp. 144–157. CIAT, Cali 1994.

    Google Scholar 

  • Rey, H.Y., Scocchi, A.M., Gonzalez, A.M., Mroginski, L.A.: Plant regeneration in Arachis pintoi (Leguminosae) through leaf culture.-Plant Cell Rep. 19: 856–862. 2000.

    Article  CAS  Google Scholar 

  • Sellars, R.M., Southward, G.M., Phillips, G.C.: Adventitious somatic embryogenesis from cultured immature zygotic embryos of peanut and soybeans.-Crop Sci. 30: 408–414, 1990.

    Article  CAS  Google Scholar 

  • Vidoz, M.L., Rey, H.Y., Gonzalez, A.M., Mroginski, L.A.: Somatic embryogenesis and plant regeneration through leaf culture in Arachis glabrata (Leguminosae).-Acta Physiol. Plant. 26: 59–66, 2004.

    Google Scholar 

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botanica del Nordeste (IBONE), C.C. 209, Corrientes, 3400, Argentina

    H. Y. Rey & L. A. Mroginski

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  1. H. Y. Rey
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  2. L. A. Mroginski
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Correspondence to H. Y. Rey.

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Rey, H.Y., Mroginski, L.A. Somatic embryogenesis and plant regeneration in diploid and triploid Arachis pintoi . Biol Plant 50, 152–155 (2006). https://doi.org/10.1007/s10535-005-0093-7

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  • DOI: https://doi.org/10.1007/s10535-005-0093-7

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