Abstract
The present study evaluated the use of γ-radiation to physically remove selective marker genes previously introduced into the soybean genome. Homozygous seeds from a transgenic soybean line carrying the gus and ahas transgenes were irradiated with γ-rays. Six plants presenting a deleted gus gene were analyzed by Southern blot to confirm removal of both ahas and gus genes. Line 1A presented an absence of the gus gene cassette and presence of the ahas gene cassette.
References
Ahloowalia, B.S., Maluszynski, M.: Induced mutations — A new paradigm in plant breeding.-Euphytica 118: 167–173, 2001.
Aragao, F.J.L., Barros, L.M.G., Brasileiro, A.C.M., Ribeiro, S.G., Smith, F.D., Sanford, J.C., Faria, J.C., Rech, E.L.: Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.-Theor. appl. Genet. 93: 142–150, 1996.
Aragao, F.J.L., Brasileiro, A.C.M.: Positive, negative and marker-free strategies for transgenic plant cell selection.-Braz. J. Plant. Physiol. 14: 1–10, 2002.
Aragao, F.J.L., Sarokin, L., Vianna, G.R., Rech, E.L.: Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean (Glycine max (L.) Merril) plants at high frequency.-Theor. appl. Genet. 101: 1–6, 2000.
Carroll, B.J., McNeil, D.L., Gresshoff, P.M.: Mutagenesis of soybean Glycine max (L.) Merr.) and the isolation of non-nodulating mutants.-Plant Sci. 47: 109–114, 1986.
Dellaporta, S.L., Wood, J., Hicks, J.B.: A plant DNA minipreparation: version II.-Plant mol. Biol. Rep. 1: 19–21, 1983.
Ebinuma, H., Komamine, A.: MAT (Multi-Auto-Transformation) vector system. The oncogenes of Agrobacterium as positive markers for regeneration and selection of marker-free transgenic plants.-In Vitro cell. dev. Biol. Plant 37: 103–113, 2001.
Jefferson, R.A., Kavanagh, T.A., Bevan, M.W.: GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.-EMBO J. 6: 3901–3907, 1987.
Kovalchuk, I., Kovalchuk, O., Hohn, B.: Genome-wide variation of the somatic mutation frequency in transgenic plants.-EMBO J. 19: 4431–4438, 2000.
Maluszynski, M., Sigurbjornsson, B. Amano, E. Sitch, L., Kamra, O.: Mutant varieties Data Bank.-Mut. Breed. Newslett. 38: 16–49, 1991.
Pavingerova, D., Briza, J., Niedermeierova, H.: Timing of transposition of Ac mobile element in potato.-Biol. Plant. 44: 347–353, 2001.
Raizada, M.N., Nan G.L.,, Walbot, V.: Somatic and germinal mobility of the Rescue Mu transposon in transgenic maize.-Plant Cell 13: 1587–1608, 2001.
Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: a Laboratory Manual.-Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.
Tulmann-Neto, A., Alves, M.C.: Induction of mutations for earliness in the soybean cultivar Parana.-Braz. J. Genet. 20: 45–50, 1997.
Vergunst, A.C., Hooykaas, P.J.J.: Recombination in the plant genome and its application in biotechnology.-Crit. Rev. Plant Sci. 18: 1–31, 1999.
Vizir, I.Y., Thorlby, G., Mulligan, B.J.: Classical mutagenesis and genetic analysis.-In: Foster, G.D., Twell, D. (ed.): Gene Isolation, Principles and Practice. Pp. 215–245. Wiley, London 1996.
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Tinoco, M.L., Vianna, G.R., Abud, S. et al. Radiation as a tool to remove selective marker genes from transgenic soybean plants. Biol Plant 50, 146–148 (2006). https://doi.org/10.1007/s10535-005-0091-9
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DOI: https://doi.org/10.1007/s10535-005-0091-9