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Secretion of a chitinase-like protein in embryogenic suspension cultures of Dactylis glomerata L.

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Biologia Plantarum

Abstract

A chitinase-like 32 kDa acidic protein with a potential chitinase activity has been identified in the medium of embryogenic suspension cultures of Dactylis glomerata L. using an antiserum raised against endochitinase EP3 from Daucus carota L. The presence of this protein discriminates between Dactylis glomerata L. embryogenic and nonembryogenic suspension cultures and thus could be possibly used as a marker for embryogenic potential.

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Abbreviations

Dicamba:

3,6-dichloro-o-anisic acid

IEF:

isoelectric focusing

PEMs:

proembryogenic masses

pI:

isoelectric point

SDS-PAGE:

sodium dodecyl sulphate polyacrylamide gel electrophoresis

SH0:

Schenk and Hildebrandt (1972) medium

SH30:

SH0 medium supplemented with 30 µM dicamba

2-D PAGE:

two-dimensional polyacrylamide gel electrophoresis

References

  • Albiach, M., Guerri, J., Moreno, P.: Multiple use of blotted polyvinylidene difluoride membranes immunostained with nitro tetrazolium.-Anal. Biochem. 221: 25–28, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Blum, H., Beier, B., Gross, H.: Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels.-Electrophoresis 8: 93–99, 1987.

    Article  CAS  Google Scholar 

  • Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Burketova, L., Stillerova, K., Feltlova, M., Sindelarova, M.: Immunohistological analysis of chemically induced proteins in sugar beet.-Biol. Plant. 47: 243–251, 2003/4.

    CAS  Google Scholar 

  • Collinge, D., Kragh, K., Mikkelsen, J., Nielsen, K., Rasmusen, U., Vad, K.: Plant chitinases.-Plant J. 3: 31–40, 1993.

    Article  PubMed  CAS  Google Scholar 

  • Conger, B.V., Hovanesian, J.C., Trigiano, R., Gray, D.: Somatic embryo ontogeny in suspension cultures of orchardgrass.-Crop Sci. 29: 448–452, 1989.

    Article  Google Scholar 

  • De Jong, A., Cordewener, J., LoSchiavo, F., Terzi, M., Vandekerckhove, J., Van Kammen, A., De Vries, S.: A carrot somatic embryo mutant is rescued by chitinase.-Plant Cell 4: 425–433, 1992.

    PubMed  Google Scholar 

  • De Jong, A., Hendriks, T., Meijer, E., Penning, M., Loschiavo, F., Terzi, M., Van Kammen, A., De Vries, S.: Transient reduction in secreted 32 kD chitinase prevents somatic embryogenesis in the carrot (Daucus carota L.) variant ts11.-Dev. Genet. 16: 332–343, 1995.

    Google Scholar 

  • De Vries, S., Booij, H., Janssens, R., Vogels, R., Saris, L., LoSchiavo, F., Terzi, M., Van Kammen, A.: Carrot somatic embryogenesis depends on the phytohormone-controlled presence of correctly glycosylated extracellular proteins.-Genes Dev. 2: 462–476, 1988.

    Google Scholar 

  • Domon, J.-M., Neutelings, G., Roger, D., David, A., David, H.: A basic chitinase-like protein secreted by Pinus caribaea acts on arabinogalactan proteins extracted from the same cell lines.-J. Plant Physiol. 156: 33–39, 2000.

    CAS  Google Scholar 

  • Egertsdotter, U., Von Arnold, S.: Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies).-Physiol. Plant. 93: 334–345, 1995.

    Article  CAS  Google Scholar 

  • Gavish, H., Vard, A., Fluhr, R.: Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins.-Planta 186: 511–517, 1992.

    Article  CAS  Google Scholar 

  • Helleboid, S., Hendriks, T., Bauw, G., Inze, D., Vasseur, J., Hilbert, J.-L.: Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins.-J. exp. Bot. 51: 1189–1200, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Kasprzewska, A.: Plant chitinases-regulation and function.-Cell. mol. Biol. Lett. 8: 809–824, 2003.

    PubMed  CAS  Google Scholar 

  • Maes, O., Coutos-Thevenot, P., Jouenne, T., Boulay, M., Guern, J.: Influence of extracellular proteins, proteases and protease inhibitors on grapevine somatic embryogenesis.-Plant Cell Tissue Organ Cult. 50: 97–105, 1997.

    CAS  Google Scholar 

  • Marek, S., Craig, R., Beuselinck, P., Karr, A.: Silver stain detection of chitinolytic enzymes after polyacrylamide gel electrophoresis.-Anal. Biochem. 230: 184–185, 1995.

    Article  PubMed  CAS  Google Scholar 

  • Mo, L., Egertsdotter, U., Von Arnold, S.: Secretion of specific extracellular proteins by somatic embryos of Picea abies is dependent on embryo morphology.-Ann. Bot. 77: 143–152, 1996.

    Article  CAS  Google Scholar 

  • Okadjima, T., Tanabe, T., Yasuda, T.: Nonurea sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-molarity buffers for the separation of protein and peptides.-Anal. Biochem. 211: 293–300, 1993.

    Google Scholar 

  • Schenk, R., Hildebrandt, A.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199–204, 1972.

    CAS  Google Scholar 

  • Tchorbadjieva, M., Odjakova, M.: An acidic esterase as a biochemical marker for somatic embryogenesis in orchardgrass (Dactylis glomerata L.) suspension cultures.-Plant Cell Rep. 20: 28–33, 2001.

    Article  CAS  Google Scholar 

  • Tchorbadjieva, M., Pantchev, I., Harizanova, N.: Two-dimensional protein pattern analysis of extracellular proteins secreted by embryogenic and non-embryogenic suspension cultures of Dactylis glomerata L.-Biotechnol. Biotechnol. Eq. 18: 20–27, 2004.

    CAS  Google Scholar 

  • Thangavelu, R., Palaniswami, A., Doraiswamy, S., Velazhahan, R.: The effect of Pseudomonas fluorescens and Fusarium oxysporum f. sp. cubense on induction of defense enzymes and phenolics in banana.-Biol. Plant. 46: 107–112, 2003.

    Article  CAS  Google Scholar 

  • Toonen, M., Schmidt, E., Van Kammen, A., De Vries, S.: Promotive and inhibitory effects of diverse arabinogalactan proteins on Daucus carota L. somatic embryogenesis.-Planta 203: 188–195, 1997.

    Article  CAS  Google Scholar 

  • Van Hengel, A., Guzzo, F., Van Kammen, A., De Vries, S.: Expression pattern of the carrot EP3 endochitinase genes in suspension cultures and in developing seeds.-Plant Physiol. 117: 43–53, 1998.

    PubMed  Google Scholar 

  • Van Hengel, A., Tadesse, Z., Immerzeel, P., Schols, H., Van Kammen, A., De Vries, S.: N-acetylglucosamine and glucosamine-containing arabinogalactan proteins control somatic embryogenesis.-Plant Physiol. 125: 1880–1890, 2001.

    PubMed  Google Scholar 

  • Wojtaszek, P., Pislewska, M., Bolwell, G., Stobiecki, M.: Secretion of stress-related proteins by suspension-cultured Lupinus albus cells.-Acta biochim. polon. 45: 281–285, 1998.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, Sofia University, Dragan Zankov str. 8, BG-1164, Sofia, Bulgaria

    M. I. Tchorbadjieva & I. Y. Pantchev

Authors
  1. M. I. Tchorbadjieva
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  2. I. Y. Pantchev
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Correspondence to M. I. Tchorbadjieva.

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Tchorbadjieva, M.I., Pantchev, I.Y. Secretion of a chitinase-like protein in embryogenic suspension cultures of Dactylis glomerata L.. Biol Plant 50, 142–145 (2006). https://doi.org/10.1007/s10535-005-0090-x

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  • DOI: https://doi.org/10.1007/s10535-005-0090-x

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