Abstract
Studies were undertaken to identify genetic relationships in three species of Typhonium and to evaluate the genetic variance within populations of Typhonium trilobatum, Typhonium roxburghii and Typhonium flagelliforme by using random amplified polymorphic DNA (RAPD) markers. A total of 193 distinct DNA fragments ranging from 0.2 to 3.2 kb, were amplified using 22 selected random decamer primers. The cluster analysis indicated that the three species of Typhonium formed two clusters: the first one consisted of T. trilobatum and T. roxburghii, the second one was represented by T. flagelliforme. A maximum similarity of 63 % was observed in T. trilobatum and T. roxburghii. T. flagelliforme shared up to 43 % similarity with T. trilobatum and T. roxburghii. The closest genetic distance was obtained within populations of different Typhonium species.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- CTAB:
-
cetyldimethyl ethylammonium bromide
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- TE:
-
Tris-EDTA buffer
References
Bai, D., Brandle, J., Reeleder, R.: Genetic diversity in North America ginseng (Panax quinquefolius L.) grown in Ontario detected by RAPD analysis.-Genome 40: 111–115, 1997.
Brown, S.M., Kresovich, S.: Molecular characterization for plant genetic resources conservation.-In: Paterson, A.H. (ed.): Genome Mapping in Plants. Pp. 85–93, Academic Press, London 1996.
Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissue.-Focus 12: 13–15, 1990.
Nei, M., Li, W.H.: Mathematical model for studying genetic variation in terms of restriction endonucleases.-Proc. nat. Acad. Sci. USA 74: 5267–5273, 1979.
Nicolson, D.H., Sivadasan, M.: Four frequently confused species of Typhonium Schott. (Araceae).-Blumea 27: 483–497, 1981.
Ortiz, A., Renaud, R., Calzada, I., Ritter, E.; Analysis of plum cultivars with RAPD markers.-J. hort. Sci. 72: 1–9, 1997.
Pal, M.D., Raychaudhuri, S.S.: Estimation of genetic variability in Plantago ovata cultivars.-Biol. Plant. 47: 459–462, 2003.
Rajaseger, G., Tan, H.T.W., Turner, I.M., Kumar, P.P.: Analysis of genetic diversity among Ixora cultivars (Rubiaceae) using Random Amplified Polymorphic DNA.-Ann. Bot. 80: 355–361, 1997.
Rohlf, F.J.: NTSYS-PC Numerical Taxonomy and Multivariate Analysis System.-Exeter Public., Setauket-New York 1995.
Rout, G.R., Bhatacharya, D., Nanda, R.M, Nayak, S., Das, P.: Evaluation of genetic relationships in Dalbergia species using RAPD markers.-Biodiversity Conserv. 12: 197–206, 2003.
Sneath, P.H.A., Sokal, R.R: Numerical Taxonomy. The Principles and Practices of Numerical Classification.-W.H. Freeman and Co., San Francisco 1973.
Virk, P.S., Ford-Lloyd, B.V., Jackson, M., Newbury, M.T.: Use of RAPD for the study of diversity within plant germplasm collections.-Heredity 74: 170–179, 1995.
Wang, J.C., Yang, K.C.: The genus Typhonium (Araceae) in Taiwan.-Bot. Bull. Acad. sin. 37: 159–163, 1996.
Whitkus, R., Doebley, J., Wendel, J.F.: Nuclear DNA markers in systematics and evolution.-In: Phillips, L., Vasil, I.K. (ed.): DNA-based Markers in Plants. Pp. 116–141, Kluwer Acad. Publ., Dordrecht 1994.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rout, G.R. Evaluation of genetic relationship in Typhonium species through random amplified polymorphic DNA markers. Biol Plant 50, 127–130 (2006). https://doi.org/10.1007/s10535-005-0086-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-005-0086-6