Abstract
In vitro organogenesis was achieved from callus derived from hypocotyl explants of Cucumis sativus L. cv. Poinsett 76. Calli were induced from hypocotyl explants excised from 7-d-old seedlings grown on Murashige and Skoog (MS) medium containing 87.64 µM sucrose, 0.8 % agar, 3.62 µM 2,4-dichlorophenoxy acetic acid and 2.22 µM 6-benzyladenine (BA). Regeneration of adventitious buds from callus (25 shoots explant−1) was achieved on MS medium supplemented with 8.88 µM BA, 2.5 µM zeatin and 10 % coconut water after two subcultures in the same medium at 30-d interval. Gibberellic acid (1.75 µM) favoured shoot elongation and indole 3-butyric acid (7.36 µM) induced rooting. Rooted plants were hardened and successfully established in soil.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenolic acetic acid
- GA3 :
-
gibberellic acid
- IAA:
-
indole acetic acid
- IBA:
-
indole-3-butyric acid
- MS medium:
-
Murashige and Skoog medium
- NAA:
-
naphthalene acetic acid
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Selvaraj, N., Vasudevan, A., Manickavasagam, M. et al. In vitro organogenesis and plant formation in cucumber. Biol Plant 50, 123–126 (2006). https://doi.org/10.1007/s10535-005-0085-7
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DOI: https://doi.org/10.1007/s10535-005-0085-7