Abstract
Oncidium cultivars gave different embryogenic responses of leaf explants when affected by auxins (2,4-D, IAA, IBA and NAA), cytokinins (2iP, BA, kinetin, TDZ and zeatin), sucrose, NaH2PO4, casein hydrolysate, peptone, and glutamine. The best embryogenic responses of cv. Sweet Sugar were at 20 g dm−3 sucrose, 85 mg dm−3 NaH2PO4 and 3 mg dm−3 kinetin, respectively. The development of somatic embryos on leaf explants of cv. Sweet Sugar was delayed for about 10 – 20 d in comparison with cv. Gower Ramsey. On growth regulator-free medium, about 40 % of leaf derived embryos of cv. Gower Ramsey were fused together in their basal parts and so called multiple-state embryos. However, under the same condition, the embryos of cv. Sweet Sugar were all in multiple-state form.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- 2iP:
-
N6-[2-isopentenyl]-adenine
- BA:
-
N6-benzyladenine
- kinetin:
-
6-furfurylaminopurine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron)
- zeatin:
-
6-[4-hydroxy-3-methylbut-2-enylamino]purine
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Su, Y.J., Chen, J.T. & Chang, W.C. Efficient and repetitive production of leaf-derived somatic embryos of Oncidium . Biol Plant 50, 107–110 (2006). https://doi.org/10.1007/s10535-005-0081-y
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DOI: https://doi.org/10.1007/s10535-005-0081-y