Abstract
Sunflower seeds behaved as chilling and freezing sensitive and also exhibited acclimation under low seed moisture content (< 1 %). At high seed moisture content (approx. 22 %) they tolerated chilling stress but failed to acclimate under freezing temperatures. Pre-imbibitional chilling (5 °C) or freezing (−5 or −10 °C) stress significantly enhanced total soluble protein (TSP) content. Chilling treatment after imbibition (in contrast to pre-imbibition) enhanced germination and this was accompanied by increase in 30, 24 and 21.9 kDa TSPs content (3 d after germination). Freezing at −5 and −10 °C suppressed seed germination and increased content of 78 and 56.2 kDa wall bound proteins. Chilling acclimation decreased 35.4, 33.9, 29.5, 23.4 and 21.4 kDa TSPs.
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Abbreviations
- SDS-PAGE:
-
sodiumdodecyl sulphate - polyacrylamide gel electrophoresis
- TSP:
-
total soluble protein
- WBP:
-
wall bound protein
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Kumar, A., Bhatla, S.C. Polypeptide markers for low temperature stress during seed germination in sunflower. Biol Plant 50, 81–86 (2006). https://doi.org/10.1007/s10535-005-0078-6
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DOI: https://doi.org/10.1007/s10535-005-0078-6