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Role of phytohormones in organogenic ability of elm multiplicated shoots

  • Published:
Biologia Plantarum

Abstract

The study presents the comparative analyses of endogenous contents of auxin (IAA), cytokinins (CKs), polyamines (PAs), and phenolic acids (PhAs) in apical and basal parts of elm multiplicated shoots with regard to the organogenic potential. The shoot-forming capacity was higher in the apical part than in the basal part. However, the timing of root formation was in the apical type of explant significantly delayed (compared with the organogenic potential of basal part). Significantly higher contents of free bases, ribosides and ribotides of isopentenyl adenine, zeatin and dihydrozeatin that were found in the apical segments, might be considered as the most important factor affecting in vitro shoot formation. The content of endogenous free IAA was approximately three times higher in the basal shoot parts than in the apical parts. The amounts of putrescine and spermidine were higher in the apical part which generally contains less differentiated tissues than the basal part of shoot. The predominant PhA in both types of explants was caffeic acid, and concentrations of other PhAs decreased in the following order: p-coumaric, ferulic, sinapic, vanillic, chlorogenic, p-hydroxybenzoic and gallic acids. The contents of all determined PhAs in their free forms and higher contents of glycoside-bound p-coumaric, ferulic and sinapic acids, precursors for lignin biosynthesis, were found in the basal parts.

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Abbreviations

CaA:

cafeic acid

pCA:

p-coumaric acid

CKs:

cytokinins

DHZ:

dihydrozeatin

DHZR:

dihydrozeatin riboside

DHZRP:

dihydrozeatin riboside monophosphate

FA:

ferulic acid

GA:

gallic acid

pHBA:

p-hydroxybenzoic acid

ChA:

chlorogenic acid

IAA:

auxin

iP:

isopentenyl adenine

iPR:

isopentenyl adenine riboside

iPRP:

isopentenyl adenine riboside monophosphate

SiA:

sinapic acid

PhAs:

phenolic acids

PAs:

polyamines

Put:

putrescine

SAVO:

0.1% NaClO3

Spd:

spermidine

Spm:

spermine

VA:

vanillic acid

Z:

zeatin

ZR:

zeatin riboside

ZRP:

zeatin riboside monophosphate

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Authors and Affiliations

  1. Forestry and Game Management Research Institute, CZ-15604, Praha 5, Zbraslav-Strnady, Czech Republic

    J. Mala

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, CZ-16502, Praha 6, Czech Republic

    A. Gaudinova, P. Dobrev, J. Eder & M. Cvikrova

Authors
  1. J. Mala
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  2. A. Gaudinova
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  3. P. Dobrev
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  4. J. Eder
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  5. M. Cvikrova
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Correspondence to M. Cvikrova.

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Mala, J., Gaudinova, A., Dobrev, P. et al. Role of phytohormones in organogenic ability of elm multiplicated shoots. Biol Plant 50, 8–14 (2006). https://doi.org/10.1007/s10535-005-0068-8

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  • DOI: https://doi.org/10.1007/s10535-005-0068-8

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