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High frequency in vitro regeneration of Lathyrus sativus L.

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  • Published:
Biologia Plantarum

Abstract

A simple and efficient protocol for high frequency plant regeneration of a grain legume grasspea (Lathyrus sativus L.) is described. Of different explant types tested epicotyl segments were most responsive. Murashige and Skoog’s (1962) medium augmented with 17.76 µM 6-benzyladenine + 10.74 µM α-naphthaleneacetic acid showed the highest percentage of direct shoot regeneration. Among cultivars IC-120487 showed the highest regeneration frequency (80 %) with maximum shoot numbers (8.2 shoots per explant) and maximum average shoot length (4.1 cm). About 78 % of the regenerated shoots were rooted in half-strength MS medium containing 2.85 µM indole-3-acetic acid. After primary hardening the plantlets were established in soil with a survival rate of 75 %.

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Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

IPA:

indole-3-propionic acid

MS medium:

Murashige and Skoog’s (1962) medium

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Plant Cell, Tissue and Organ Culture Facility, Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Orissa, 751004, India

    D. P. Barik, U. Mohapatra & P. K. Chand

Authors
  1. D. P. Barik
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  2. U. Mohapatra
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  3. P. K. Chand
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Correspondence to P. K. Chand.

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Barik, D.P., Mohapatra, U. & Chand, P.K. High frequency in vitro regeneration of Lathyrus sativus L.. Biol Plant 49, 637–639 (2005). https://doi.org/10.1007/s10535-005-0066-x

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  • DOI: https://doi.org/10.1007/s10535-005-0066-x

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