Abstract
Using 2-D electrophoresis, we analyzed proteins from transgenic rice overexpressing gibberellin acid (GA) catabolic enzyme, GA2-oxidase. These results indicate eight specific proteins differentially expressed in the transformed rice stems of T1 generation, but non in case of T2 generation. Proteins isolated from different stages of leaves of T1 generation showed no significant differences, except one-month-old leaf, where five differentially expressed proteins are visible.
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Abbreviations
- GA:
-
gibberellin
- IEF:
-
isoelectric focusing
- PAGE:
-
polyacrylamide gel electrophoresis
References
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This work was supported in part by a research project of an Identification and Analysis of Proteins for Gene Discovery and Elucidation of Functions of Useful Genes in Rice Genome from Ministry of Agriculture, Forestry and Fisheries, and also supported by a part of grant from the Program of Basic Research Activities for Innovative Biosciences. Martin Hajduch was supported by European Commission’s specific research and technological development program “Confirming the International Role of Community Research” 1998-2002 (EU Fellowship to Japan). The authors are solely responsible for the content of this paper and it does not represent the opinion of the Community, and the Community is not responsible for any of use that might be made of date appearing herein.
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Hajduch, M., Tanaka, H., Morinaka, Y. et al. Protein analysis of dwarfed transgenic rice plants overexpressing GA2-oxidase gene. Biol Plant 49, 621–624 (2005). https://doi.org/10.1007/s10535-005-0061-2
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DOI: https://doi.org/10.1007/s10535-005-0061-2