Abstract
Ethylene concentration in the culture tubes of peach rootstock regenerants of three genotypes (Cadaman, GF-677, Myrobalan 29C) was increased by the inclusion of 20 µM salicylic acid (SA), methionine (METH) and ethephon (ETH) in the MS medium whereas it was decreased in regenerants exposed up to 20 µM AgNO3. In leaves of the regenerants the increase of ethylene concentration was accompanied with an increase of non-enzymatic antioxidant activity while remarkable genotype-depended changes in the activities of catalase, peroxidase and their isoenzymes were recorded suggesting that ethylene accumulation imposes oxidative stress responses. However, the results showed that some differences could be observed in the activity of isoenzymes in regenerants exposed to SA in respect to METH and ETH-treated ones.
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Abbreviations
- CAT:
-
catalase
- DMAB:
-
3-dimethylaminobenzoic acid
- ETH:
-
ethephon
- FRAP:
-
ferric reducing antioxidant power
- MBTH:
-
3 methyl-2-benzothiazolinonhydrazon hydrochloride hydrate
- METH:
-
methionine
- MS medium:
-
Murashige and Skoog medium
- PAGE:
-
polyacrylamide gel electrophoresis
- POD:
-
peroxidase
- ROS:
-
reactive oxygen species
- SA:
-
salicylic acid
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A. Molassiotis is grateful to the State Scholarship’s Foundation of Greece for a fellowship during this work.
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Molassiotis, A., Diamantidis, G., Therios, I. et al. Effects of salicylic acid on ethylene induction and antioxidant activity in peach rootstock regenerants. Biol Plant 49, 609–612 (2005). https://doi.org/10.1007/s10535-005-0057-y
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DOI: https://doi.org/10.1007/s10535-005-0057-y