Abstract
The leaves of pepper (Capsicum anuum L.) were inoculated with Phytophthora capsici Leonian 3 d after treatment with acibenzolar-S-methylbenzo [1,2,3]thiadiazole-7-carbothioic acid-S-methyl ester (ASM) and resistance to Phytophthora blight disease was investigated. Results showed that P. capsici was significantly inhibited by ASM treatment by up to 45 % in planta. The pepper plants responded to ASM treatments by rapid and transient induction of L-phenylalanine ammonia-lyase (PAL), increase in total phenol content and activities of chitinase and β-1,3-glucanase. No significant increases in enzyme activities were observed in water-treated control plants compared with the ASM-treated plants. Therefore it may be suggested that ASM induces defense-related enzymes, PAL activity, PR proteins and phenol accumulation in ASM-treated plants and contribute to enhance resistance against P. capsici.
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Abbreviations
- AMS:
-
acibenzolar-S-methylbenzo[1,2,3]thiadiazole-7-carbothioic acid-S-methyl ester, dai - days after inoculation
- IR:
-
induced resistance
- PAL:
-
L-phenylalanine ammonialyase
- PR:
-
pathogenesis-related
- SA:
-
salicylic acid
- SAR:
-
systemic aquired resistance
References
Anfoka, G.H.: Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato (Lycopersicon esculentum Mill. cv.Volledung) to cucumber mosaic virus.-Crop Protect. 19: 401–405, 2000.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Burketova, L., Sindelarova, M., Sindelar, L.: Benzothiadiazole as an inducer of β-1,3-glucanase and chitinase isozymes in sugar beet.-Biol. Plant. 42: 279–287, 1999.
Burketova, L., Stillerova, K., Feltlova, M., Sindelarova, M.: Immunolohistological analysis of chemically induced proteins in sugar beet.-Biol. Plant. 47: 243–251, 2003.
Cohen, Y., Niederman, T., Mosinger, E., Fluhr, R.: β-Aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycopersicon esculentum L.) plants and resistance to late blight infection caused by Phytophthora infestans.-Plant Physiol. 104: 59–66, 1994.
Cole, D.L.: The efficacy of acibenzolar-S-methyl, an inducer of systemic acquired resistance against bacterial and fungal diseases of tobacco.-Crop Protect. 18: 267–273, 1999.
Conrath, U., Thulke, O., Katz, V., Schwindling, S., Kohler, A.: Priming as a mechanism in induced systemic resistance of plants.-Eur. J. Plant Pathol. 107: 113–119, 2001.
Dickerson, D.P., Pascholati, S.F., Hagerman, A.E., Butler, L.G., Nicholson, R.L.: Phenylalaninelyase and hydroxycinnamate; CoA ligase in maize mesocotyls inoculated with Helminthosporium carbonum.-Physiol. Plant Pathol. 25: 111–123, 1984.
Dixon, A.R., Lamb, C.J.: Molecular communication in interaction between plants and microbial pathogens.-Annu. Rev. Plant Physiol. Plant mol. Biol. 41: 339–367, 1990.
Doubrava, N.S., Dean, R.A., Kuc, J.: Induction of systemic resistance to antracnosis caused by Collectotrichum lagenarium in cucumber by oxalate and extracts of spinach and rhubarb leaves.-Physiol. mol. Plant Pathol. 33: 69–79, 1988.
Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M.G., Meier, B., Dincher, S., Staub, T., Uknes, S., Metraux, J.-P., Kessmann, H., Ryals, J.: A benzothiadiazole derivative induces systemic acquired resistance in tobacco.-Plant J. 10: 61–70, 1996.
Gorlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K.H., Oostendorp, M., Staub, T., Ward, E., Kessmann, H., Ryals, J.: Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.-Plant Cell 8: 629–643, 1996.
Hammerschmidt, R., Kuc, J.: Induced Resistance to Disease in Plants.-Kluwer Academic Publishers, Dordrecht 1995.
Heath, M.C.: Reactions of nonsuscepts to fungal pathogens.-Annu. Rev. Phytopathol. 18: 211–236, 1980.
Lawton, K.A., Friedrich, L., Hunt, M., Weymann, K., Delaney, T., Kessmann, H., Staub, T., Ryals, J.: Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.-Plant J. 10: 71–82, 1996.
Lopez, A.M.Q., Lucas, J.A.: Effects of plant defence activators on anthracnose disease of cashew.-Eur. J. Plant Pathol. 108: 409–420, 2002.
Matern, U., Grimming, B.: Natural phenols as stress metabolites.-Acta Hort. 381: 448–462, 1994.
Matern, U., Kneusel, R.: Phenolic compounds in plant disease resistance.-Phytoparasitica 16: 153–170, 1988.
Oostendorp, M., Kunz, W., Dietrich, B., Staub, T.: Induced disease resistance in plants by chemicals.-Eur. J. Plant Pathol. 107: 19–28, 2001.
Reuveni, M., Zahavi, T., Cohen, Y.: Controlling downy mildew (Plasmopara viticola) in field-grown grapevine by β-aminobutyric acid (BABA).-Phytoparasitica 29: 125–133, 2001.
Sid, A., Ezziyyani, M., Egea-Gilabert, C., Candela, M.E.: Selecting bacterial strains for use in the biocontrol of diseases caused by Phytophthora capsici and Alternaria alternata in sweet pepper plants.-Biol. Plant. 47: 569–574, 2003.
Siegrist, J., Glenewinkel, D., Kolle, C., Schmidtke, M.: Chemical induced resistance in green bean against bacterial and fungal pathogens.-J. Plant Dis. Protect. 104: 599–610, 1997.
Stadnik, M.J., Buchenauer, H.: Inhibition of phenylalanine ammonia-lyase suppresses the resistance induced by benzothiadiazole in wheat to Blumeria graminis f. sp. tritici.-Physiol. mol. Plant Pathol. 57: 25–34, 2000.
Stintzi, A., Heitz, T., Prasad, V., Wiedemann-Merdinoghi, S., Kauffmann, S., Geoffroy, P., Legrand, M., Fritig, B.: Plant ‘pathogenesis-related’ proteins and their role in defence against pathogens.-Biochemistry 75: 687–706, 1993.
Sunwoo, J.Y., Lee, Y.K., Hwang, B.K.: Induced resistance against Phytophthora capsici in pepper plants in response to DL-β-amino-n-butyric acid.-Eur. J. Plant Pathol. 102: 663–670, 1996.
Suo, Y., Leung, D.W.M.: Elevation of extracellular β-1,3-glucanase and chitinase activities in rose in response to treatment with acibenzolar-S-methyl and infection by D. rosae.-J. Plant Physiol. 158: 971–976, 2001.
Van Loon, L.C.: Induced resistance in plants and the role of pathogenesis-related proteins.-Eur. J. Plant Pathol. 103: 753–765, 1997. 1997
Wirth, S.J., Wolf, G.A.: Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity.-J. Micromethods 12: 197–205, 1990.
Wirth, S.J., Wolf, G.A.: Micro-plate colorimetric assay for endo-acting cellulase, xylanase, chitinase β-1,3-glucanase and amylase extracted from forest soil horizons.-Soil Biol. Biochem. 24: 511–519, 1992.
Zhu, Q., Maker, E.A., Masond, S., Dixon, R.E., Lamb, C.: Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase in transgenic tobacco.-Bio-Technology 12: 807–812, 1994.
Zieslin, N., Ben-Zaken, R.: Peroxidase activity and presence of phenolic substances in peduncles of rose flowers.-Plant Physiol. Biochem. 31: 333–339, 1993.
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Baysal, O., Turgut, C. & Mao, G. Acibenzolar-S-methyl induced resistance to Phytophthora capsici in pepper leaves. Biol Plant 49, 599–604 (2005). https://doi.org/10.1007/s10535-005-0055-0
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DOI: https://doi.org/10.1007/s10535-005-0055-0