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Seed protein electrophoresis of some cultivated and wild species of Chenopodium

Abstract

Seed protein profiles of 40 cultivated and wild taxa of Chenopodium have been compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The relative similarity between various taxa, estimated by Jaccard’s similarity index and clustered in UPGMA dendrogram, is generally in accordance with taxonomic position, crossability relationships and other biochemical characters. Eight accessions of C. quinoa studied are clustered together and show genetic similarity with closely related C. bushianum and C. berlandieri subsp. nuttalliae. The taxa included under C. album complex are clustered in two groups which show that these taxa are a heterogenous assemblage and their taxonomic affinities need a reassessment. Other wild species studied are placed in the dendrogram more or less according to their taxonomic position.

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Abbreviations

NTSYS-PC:

numerical taxonomy and multivariate analysis system

OTU:

operational taxonomic unit

PAGE:

polyacrylamide gel electrophoresis

SDS:

sodiumdodecyl sulphate

UPGMA:

unweighted pair-group method

References

  1. Ahmad, F., Slinkard, A.E.: Genetic relationships in the genus Cicer L. as revealed by polyacrylamide gel electrophoresis of seed storage proteins.-Theor. appl. Genet. 84: 688–692, 1992.

    Article  Google Scholar 

  2. Bera, B., Mukherjee, K.K.: Phenotypic variability in Chenopodium album.-Nucleus 30: 50–53, 1987.

    Google Scholar 

  3. El Naggar, S.M.: Implications of seed proteins in Brassicaceae systematics.-Biol. Plant. 44: 547–553, 2001.

    Article  Google Scholar 

  4. Gangopadhyay, G., Das, S., Mukherjee, K.K.: Speciation in Chenopodium in West Bengal, India.-Genet. Res. Crop Evol. 49: 503–510, 2002.

    Article  Google Scholar 

  5. Ghafoor, A., Ahmad, Z., Qureshi, A.S., Bashir, M.: Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE.-Euphytica 123: 367–378, 2002.

    Article  Google Scholar 

  6. Giusti, L.: [El genero Chenopodium in Argentina I. Numero de cromosomas.]-Darwiniana 16: 98–105, 1970. [In Span.]

    Google Scholar 

  7. Heiser, C.B., Nelson, D.C.: On the origin of cultivated chenopods (Chenopodium).-Genetics 78: 503–505, 1974.

    PubMed  Google Scholar 

  8. Jha, S.S., Ohri, D.: Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeon pea) and its wild relatives based on seed protein profiles.-Genet. Res. Crop Evol. 43: 275–281, 1996.

    Article  Google Scholar 

  9. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4-Nature 227: 680–685, 1970.

    PubMed  Google Scholar 

  10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randal, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.

    PubMed  Google Scholar 

  11. Mehra, P.N., Malik, C.P.: Cytology of some Indian Chenopodiaceae.-Caryologia 16: 67–84, 1963.

    Google Scholar 

  12. Mosyakin, S.L., Clemants, S.E.: New infrageneric taxa and combinations in Chenopodium L. (Chenopodiaceae).-Novon 6: 398–403, 1996.

    Google Scholar 

  13. Mukherjee, K.K.: A comparative study of two cytotpes of Chenopodium album in West Bengal, India.-Can. J. Bot. 64: 754–759, 1986.

    Google Scholar 

  14. Nath, P., Ohri, D., Jha, S.S., Pal, M.: Seed protein electrophoresis of wild and cultivated species of Celosia (Amaranthaceae).-Genet. Res. Crop Evol. 44: 241–245, 1997.

    Article  Google Scholar 

  15. Pal, M., Shukla, S.: A hexaploid grain chenopod from Eastern Himalayas.-Newslett. Himalayan Bot. 8: 12–14, 1990.

    Google Scholar 

  16. Partap, T., Joshi, B.D., Galwey, N.W.: Chenopods: Chenopodium spp. Promoting the Conservation and Use of Underutilized and Neglected Crops.-Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome 1998.

    Google Scholar 

  17. Risi, J., Galwey, N.W.: The Chenopodium grains of the Andes: Inca crops for modern agriculture.-Adv. appl. Biol. 10: 146–206, 1984.

    Google Scholar 

  18. Ruas, P.M, Bonifacio, A., Ruas, C.F., Fairbanks, D.J., Anderson, W.R.: Genetic relationships among 19 accessions of six species of Chenopodium L. by random amplified polymorphic DNA fragments (RAPD).-Euphytica 105: 25–32, 1999.

    Article  Google Scholar 

  19. Scott, A.J.: A review of the classification of Chenopodium L. and related genera (Chenopodiaceae).-Bot. Jahrb. Syst. 100: 205–220, 1978.

    Google Scholar 

  20. Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy.-W.H. Freeman, San Francisco 1973.

    Google Scholar 

  21. Vladova, R., Pandeva, R., Petcolicheva, K.: Seed storage proteins in Capsicum annum cultivars.-Biol. Plant. 43: 291–295, 2000.

    Article  Google Scholar 

  22. Walters, T.W.: Electrophoretic evidence for the evolutionary relationship of the tetraploid Chenopodium berlandieri to its putative diploid progenitors.-Selbyana 10: 36–55, 1987.

    Google Scholar 

  23. Wilson, H.D.: Genetic control and distribution of leucine aminopeptidase in the cultivated chenopods (Chenopodiaceae) and related weed taxa.-Biochem. Genet. 14: 913–919, 1976.

    Article  PubMed  Google Scholar 

  24. Wilson, H.D.: Artificial hybridization among species of Chenopodium sect. Chenopodium.-Syst. Bot. 5: 253–263, 1980.

    Google Scholar 

  25. Wilson, H.D.: Genetic variations among South American populations of tetraploid Chenopodium sect. Chenopodium subsect. Cellulata.-Syst. Bot. 6: 380–398, 1981.

    Google Scholar 

  26. Wilson, H.D.: Allozyme variation and morphological relationships of Chenopodium hircinum Schrader (s.lat.).-Syst. Bot. 13: 215–228, 1988a.

    Google Scholar 

  27. Wilson, H.D.: Quinua biosystematics I. Domesticated populations.-Econ. Bot. 42: 461–477, 1988b.

    Google Scholar 

  28. Wilson, H.D.: Quinua biosystematics II. Free living populations.-Econ. Bot. 42: 478–494, 1988c.

    Google Scholar 

  29. Wilson, H.D.: Quinoa and relatives (Chenopodium sect. Chenopodium subsect. Cellulata).-Econ. Bot. 44: 92–110, 1990.

    Google Scholar 

  30. Wilson, H.D., Heiser, C.B.: The origin and evolutionary relationships of ‘Huazontle’ (Chenopodium nuttalliae Safford) domesticated chenopod of Mexico.-Amer. J. Bot. 66: 198–206, 1979.

    Google Scholar 

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Correspondence to D. Ohri.

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Bhargava, A., Rana, T.S., Shukla, S. et al. Seed protein electrophoresis of some cultivated and wild species of Chenopodium . Biol Plant 49, 505–511 (2005). https://doi.org/10.1007/s10535-005-0042-5

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Additional key words

  • genetic diversity
  • Jaccard’s similarity index
  • SDS-PAGE
  • taxonomy
  • UPGMA dendrogram