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Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression

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Biologia Plantarum


We have cloned and characterized the expression of Crocus sativus AGAMOUS1 (CsAG1), a putative C-type MADS-box gene homologous to AGAMOUS (AG) from a triploid monocot species crocus (Crocus sativus L.). The typical domain structure of MIKC-type plant MADS proteins was identified. Phylogenetic analysis of the deduced amino acid sequence indicated that the isolated gene forms a clade with the AGAMOUS homologs from the monocots Hyacinthus orientalis and Phalaenopsis equestris. A differential splicing event altering the amino acid sequence at the C terminus was identified, leading to the formation of two mRNAs differing ten nucleotides in size. The presence of both differentially spliced transcripts was restricted only to mature crocus flowers and particularly to stamens and carpels.

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  • Alvarez-Buylla, E.R., Pelaz, S., Liljegren, S.J., Gold, S.E., Burgeff, C., Ditta, G.S., Ribas de Pouplana, L., Martinez-Castilla, L., Yanofsky, M.F.: An ancestral MADS-box gene duplication occurred before the divergence of plants and animals.-Proc. nat. Acad. Sci. USA 97: 5328–5333, 2000.

    Article  PubMed  Google Scholar 

  • Angenent, G.C., Franken, J., Busscher, M., Colombo, L., Van Tunen, A.J.: Petal and stamen formation in petunia is regulated by the homeotic gene fbp1.-Plant J. 4: 101–112, 1993.

    Article  PubMed  Google Scholar 

  • Boss, P.K., Vivier, M., Matsumoto, S., Dry, I.B., Thomas, M.R.: A cDNA from grapevine (Vitis vinifera L.), which shows homology to AGAMOUS and SHATTERPROOF, is not only expressed in flowers but also throughout berry development.-Plant mol. Biol. 45: 541–553, 2001.

    Article  PubMed  Google Scholar 

  • Ferrario, S., Immink, R.G., Angenent, G.C.: Conservation and diversity in flower land.-Curr. Opinion Plant Biol. 7: 84–91, 2004.

    Article  Google Scholar 

  • Immink, R.G., Gadella, T.W., Jr., Ferrario, S., Busscher, M., Angenent, G.C.: Analysis of MADS box protein-protein interactions in living plant cells.-Proc. nat. Acad. Sci. USA 99: 2416–2421, 2002.

    Article  PubMed  Google Scholar 

  • Kang, H.G., Noh, Y.S., Chung, Y.Y., Costa, M.A., An, K., An, G.: Phenotypic alterations of petal and sepal by ectopic expression of a rice MADS box gene in tobacco.-Plant mol. Biol. 29: 1–10, 1995.

    Article  PubMed  Google Scholar 

  • Kempin, S.A., Mandel, M.A., Yanofsky, M.F.: Conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene NAG1.-Plant Physiol. 103: 1041–1046, 1993.

    Article  PubMed  Google Scholar 

  • Kim, S.H., Mizuno, K., Fujimura, T.: Isolation of MADS-box genes from sweet potato (Ipomoea batatas (L.) Lam.) expressed specifically in vegetative tissues.-Plant Cell Physiol. 43: 314–322, 2002.

    Article  PubMed  Google Scholar 

  • Kitahara, K., Matsumoto, S.: Rose MADS-box genes ‘MASAKO C1 and D1’ homologous to class C floral identity genes.-Plant Sci. 151: 121–134, 2000.

    Article  PubMed  Google Scholar 

  • Kumar, S., Tamura, K., Jakobsen, I.B., Nei, M.: MEGA2: molecular evolutionary genetics analysis software.-Bioinformatics 17: 1244–1245, 2001.

    Article  PubMed  Google Scholar 

  • Lorkovic, Z.J., Wieczorek, Kirk, D.A., Lambermon, M.H., Filipowicz, W.: Pre-mRNA splicing in higher plants.-Trends Plant Sci. 5: 160–167, 2000.

    Article  PubMed  Google Scholar 

  • Mandel, M.A., Bowman, J.L., Kempin, S.A., Ma, H., Meyerowitz, E.M., Yanofsky, M.F.: Manipulation of flower structure in transgenic tobacco.-Cell 71: 133–143, 1992.

    Article  PubMed  Google Scholar 

  • Nam, J., dePamphilis, C.W., Ma, H., Nei, M.: Antiquity and evolution of the MADS-box gene family controlling flower development in plants.-Mol. Biol. Evol. 20: 1435–1447, 2003.

    Article  PubMed  Google Scholar 

  • Pnueli, L., Hareven, D., Rounsley, S.D., Yanofsky, M.F., Lifschitz, E.: Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants.-Plant Cell 6: 163–173, 1994.

    Article  PubMed  Google Scholar 

  • Rigola, D., Pe, M.E., Fabrizio, C., Me, G., Sari-Gorla, M.: CaMADS1, a MADS box gene expressed in the carpel of hazelnut.-Plant mol. Biol. 38: 1147–1160, 1998.

    Article  PubMed  Google Scholar 

  • Rounsley, S.D., Ditta, G.S., Yanofsky, M.F.: Diverse roles for MADS box genes in Arabidopsis development.-Plant Cell 7: 1259–1269, 1995.

    Article  PubMed  Google Scholar 

  • Saitou, N., Nei, M.: The neighbor-joining method: a new method for reconstructing phylogenetic trees.-Mol. Biol. Evol. 4: 406–425, 1987.

    PubMed  Google Scholar 

  • Schmidt, R.J., Veit, B., Mandel, M.A., Mena, M., Hake, S., Yanofsky, M.F.: Identification and molecular characterization of ZAG1, the maize homolog of the Arabidopsis floral homeotic gene AGAMOUS.-Plant Cell 5: 729–737, 1993.

    Article  PubMed  Google Scholar 

  • Theissen, G.: Development of floral organ identity: stories from the MADS house.-Curr. Opinion Plant Biol. 4: 75–85, 2001.

    Google Scholar 

  • Theissen, G., Becker, A., Di Rosa, A., Kanno, A., Kim, J.T., Munster, T., Winter, K.U., Saedler, H.: A short history of MADS-box genes in plants.-Plant mol. Biol. 42: 115–149, 2000.

    Article  PubMed  Google Scholar 

  • Theissen, G., Strater, T., Fischer, A. and Saedler, H.: Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize.-Gene 156: 155–166, 1995.

    Article  PubMed  Google Scholar 

  • Thompson, J.D., Higgins, D.G., Gibson, T.J.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.-Nucl. Acids Res. 22: 4673–4680, 1994.

    PubMed  Google Scholar 

  • Tsaftaris, A.S., Pasentsis, K., Iliopoulos, I., Polidoros, A.N.: Isolation of three homologous AP1-like MADS-box genes in crocus (Crocus sativus L.) and characterization of their expression.-Plant Sci. 166: 1235–1243, 2004.

    Article  Google Scholar 

  • Tsuchimoto, S., Van der Krol, A.R., Chua, N.H.: Ectopic expression of pMADS3 in transgenic petunia phenocopies the petunia blind mutant.-Plant Cell 5: 843–853, 1993.

    Article  PubMed  Google Scholar 

  • Van der Linden, C.G., Vosman, B., Smulders, M.J.: Cloning and characterization of four apple MADS box genes isolated from vegetative tissue.-J. exp. Bot. 53: 1025–1036, 2002.

    Article  PubMed  Google Scholar 

  • Weigel, D., Meyerowitz, E.M.: The ABCs of floral homeotic genes.-Cell 78: 203–209, 1994.

    Article  PubMed  Google Scholar 

  • Yanofsky, M.F., Ma, H., Bowman, J.L., Drews, G.N., Feldmann, K.A., Meyerowitz, E.M.: The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors.-Nature 346: 35–39, 1990.

    Article  PubMed  Google Scholar 

  • Yu, D., Kotilainen, M., Pollanen, E., Mehto, M., Elomaa, P., Helariutta, Y., Albert, V.A., Teeri, T.H.: Organ identity genes and modified patterns of flower development in Gerbera hybrida (Asteraceae).-Plant J. 17: 51–62, 1999.

    Article  PubMed  Google Scholar 

  • Zhang, P., Tan, H.T., Pwee, K.H., Kumar, P.P.: Conservation of class C function of floral organ development during 300 million years of evolution from gymnosperms to angiosperms.-Plant J. 37: 566–577, 2004.

    Article  PubMed  Google Scholar 

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Correspondence to A. S. Tsaftaris.

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Tsaftaris, A.S., Pasentsis, K. & Polidoros, A.N. Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression. Biol Plant 49, 499–504 (2005).

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