Abstract
The activities of sucrose phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI) were measured in callus cultures of four Mexican sugarcane cultivars (Saccharum spp.) with a different capacity to accumulate sucrose in stem parenchyma cells. The results indicated that sucrose accumulation in callus was positively correlated to the activity of SPS and SUSY and negatively to the activity of SAI and NI while SPS explained most of the variation found for sucrose accumulation and NI least.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS medium:
-
Murashige and Skoog medium
- NI:
-
neutral invertase
- SAI:
-
soluble acid invertase
- SPS:
-
sucrose-phosphate synthase
- SUSY:
-
sucrose synthase
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The research was funded by the department of Biotechnology and Bioengineering CINVESTAV Mexico City, and F. G.-M. received grant-aided support from CONACyT, Mexico.
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Gutierrez-Miceli, F.A., Rodriguez-Mendiola, M.A., Ochoa-Alejo, N. et al. Sucrose accumulation and enzyme activities in callus culture of sugarcane. Biol Plant 49, 475–479 (2005). https://doi.org/10.1007/s10535-005-0034-5
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DOI: https://doi.org/10.1007/s10535-005-0034-5