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Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects

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Biologia Plantarum

Abstract

A rapid plantlet regeneration system for Perilla frutescens was established from cotyledon and hypocotyl explants. A maximum of 91.06 % cotyledon and 76.4 % hypocotyl explants could directly produce shoots (3.09 ± 0.18 shoots per explants) on Murashige and Skoog (MS) medium. The optimum hormone combinations were 4.44 μM 6-benzylaminopurine (BA) for cotyledon and 2.22 μM BA + 2.85 μM indole-3-acetic acid (IAA) for hypocotyls. Rooting was induced on half-strength hormone-free MS medium. After transplantation to soil, approximate 80 % of the regenerated plantlets could survive, flower and fruit. Moreover, some morphological abnormalities were found among the regenerated plants.

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Abbreviations

BA:

6-benzylaminopurine

IAA:

indole-3-acetic acid

NAA:

α-naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS medium:

Murashige and Skoog medium

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Authors and Affiliations

  1. The Institute of Cell Biology, Lanzhou University, Lanzhou, 730000, P.R. China

    T. Zhang, X. Y. Wang & Z. Y. Cao

  2. Gansu Academy of Agricultural Science, Lanzhou, 730070, P.R. China

    T. Zhang

  3. School of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, P.R. China

    Z. Y. Cao

Authors
  1. T. Zhang
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  2. X. Y. Wang
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  3. Z. Y. Cao
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Correspondence to X. Y. Wang.

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Zhang, T., Wang, X.Y. & Cao, Z.Y. Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects. Biol Plant 49, 423–426 (2005). https://doi.org/10.1007/s10535-005-0020-y

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  • DOI: https://doi.org/10.1007/s10535-005-0020-y

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