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Gene regulation and ionome homeostasis in rice plants in response to arsenite stress: potential connection between transcriptomics and ionomics

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Abstract

Ionomics and transcriptomics were applied to demonstrate response of rice to arsenite [As(III)] stress in the current study. Rice plants were cultured in nutrient solutions treated with 0, 100 and 500 μg/L As(III) coded as CK, As1 and As5, respectively. The rice ionomes exhibited discriminatory response to environmental disturbances. Solid evidence of the effects of As(III) stress on binding, transport or metabolism of P, K, Ca, Zn and Cu was obtained in this work. Differentially expressed genes (DEGs) in the shoots were identified in three datasets: As1 vs CK, As5 vs CK and As5 vs As1. DEGs identified simultaneously in two or three datasets were selected for subsequent interaction and enrichment analyses. Upregulation of genes involved in protein kinase activity, phosphorus metabolic process and phosphorylation were detected in the rice treated with As(III), resulting in the maintenance of P homeostasis in the shoots. Zn and Ca binding genes were up-regulated since excess As inhibited the translocation of Zn and Ca from roots to shoots. Increased expression of responsive genes including HMA, WRKY, NAC and PUB genes conferred As tolerance in the rice plants to cope with external As(III) stress. The results suggested that As(III) stress could disturb the uptake and translocation of macro and essential elements by rice. Plants could regulate the expression of corresponding genes to maintain mineral nutrient homeostasis for essential metabolic processes.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 41977351) and the Natural Science Foundation of Hunan Province, China (No. 2021JJ31094).

Funding

This work was financially supported by the National Natural Science Foundation of China (No. 41977351) and the Natural Science Foundation of Hunan Province, China (No. 2021JJ31094).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    Guobing Lin, Xiaoman He & Lin Wang

  2. College of Forestry, Henan Agriculture University, Zhengzhou, 450002, China

    Li Ma

  3. Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China

    Jie Tang

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  1. Guobing Lin
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  2. Li Ma
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Contributions

GL: Methodology, Investigation, Visualization, Writing- Original draft preparation. LM: Investigation, Visualization. XH: Investigation. JT: Methodology, Writing—Review & Editing, Resources. LW: Supervision, Writing- Original draft preparation, Writing—Review & Editing, Funding acquisition.

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Correspondence to Jie Tang or Lin Wang.

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Lin, G., Ma, L., He, X. et al. Gene regulation and ionome homeostasis in rice plants in response to arsenite stress: potential connection between transcriptomics and ionomics. Biometals 36, 1157–1169 (2023). https://doi.org/10.1007/s10534-023-00510-z

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