Abstract
Selenium (Se) plays an essential role in the growth of fish and performs its physiological functions mainly through incorporation into selenoproteins. Our previous studies suggested that the selenoprotein W gene (selenow) is sensitive to changes in dietary Se in rainbow trout. However, the molecular characterization and tissue expression pattern of selenow are still unknown. Here, we revealed the molecular characterization, the tissue expression pattern of rainbow trout selenow and analyzed its response to dietary Se. The open reading frame (ORF) of the selenow gene was composed of 393 base pairs (bp) and encodes a 130-amino-acid protein. The 3′ untranslated region (UTR) was 372 bp with a selenocysteine insertion sequence (SECIS) element. Remarkably, the rainbow trout selenow gene sequence was longer than those reported for mammals and most other fish. A β1-α1-β2-β3-β4-α2 pattern made up the secondary structure of SELENOW. Furthermore, multiple sequence alignment revealed that rainbow trout SELENOW showed a high level of identity with SELENOW from Salmo salar. In addition, the selenow gene was ubiquitously distributed in 13 tissues with various abundances and was predominantly expressed in muscle and brain. Interestingly, dietary Se significantly increased selenow mRNA expression in muscle. Our results highlight the vital role of selenow in rainbow trout muscle response to dietary Se levels and provide a theoretical basis for studies of selenow.
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Acknowledgements
This work was financially supported by the National Key R&D Program (Grant Number: 2019YFD0900303) and the Fundamental Research Funds for the Central Universities (Grant Numbers: 2662021SPPY001 and 11900022414).
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CL: conceptualization, investigation, formal analysis, writing-original draft. FZ, ZT and GZ: methodology, formal analysis, writing—review & editing. YY, PX, XH, LW, FY and ZY: methodology, formal analysis. XZ: writing-review & editing, supervision, project administration, funding acquisition. All the authors read and approved the manuscript.
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Liao, C., Zhang, F., Teng, Z. et al. Molecular characterization and expression analysis of selenoprotein W gene in rainbow trout (Oncorhynchus mykiss) with dietary selenium levels. Biometals 35, 1359–1370 (2022). https://doi.org/10.1007/s10534-022-00451-z
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DOI: https://doi.org/10.1007/s10534-022-00451-z