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Green synthesis of selenium nanoparticles using Rosmarinus officinalis and investigated their antimicrobial activity

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Abstract

The interest of many has been attracted by plant-mediated synthesizing procedures for nanoparticles since they provide certain qualities including being cost-effective, quick, and compatible with the environment. In this regard, this work introduces the production of selenium-nanoparticles (Se-NPs) in a biological manner utilizing aqueous extracts of Rosmarinus officinalis (R. officinalis). Production of Se-NPs was confirmed using UV–visible (UV–Vis) spectrophotometry. Also, dynamic light scattering (DLS) analysis was used for determination particle size distribution, while we distinguished the identification of crystalline construction of nanoparticles through the means of X-ray diffraction (XRD) pattern, DLS, and transmission electron microscopy (TEM) examination indicated that Se-NPs are often spherical with a size about 20 to 40 nm. The minimum inhibitory concentration (MIC) of the synthesized Se-NPs by R. officinalis extract against Mycobacterium tuberculosis (M. tuberculosis), Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa) was 256, 16, 32, 128, and 64 µg/mL, respectively. The synthesized Se-NPs had no significant effect on Mycobacterium simiae (M. simiae) and had exhibited a strong antimicrobial functionality towards the gram-positive and gram-negative bacteria and can stand as a potent antibacterial agent.

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Abbreviations

Na2SeO3 :

Sodium selenite

Se-NPs:

Selenium-nanoparticles

REMA:

Resazurin microtiter assay

NA:

Nutrient agar

MIC:

Minimum inhibitory concentration

TSB:

Trypticase soy broth

OADC:

Oleic acid, albumin, dextrose, and catalase

UV–Vis:

Ultraviolet–visible

XRD:

X-ray diffraction

TEM:

Transmission electron microscopy

FT-IR:

Fourier transform infrared

DLS:

Dynamic light scattering

R. officinalis :

Rosmarinus officinalis

M. tuberculosis :

Mycobacterium tuberculosis

S. aureus :

Staphylococcus aureus

S. mutans :

Streptococcus mutans

E. coli :

Escherichia coli

P. aeruginosa :

Pseudomonas aeruginosa

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Acknowledgements

The technical support of this work was provided by Mashhad University of Medical Sciences based on the MSc Thesis of Ms. F. Adibian.

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Authors and Affiliations

  1. Antimicrobial Resistance Research Centre, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Adibian, Roya Saddat Ghaderi & Saman Soleimanpour

  2. Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Adibian, Roya Saddat Ghaderi, Masoud Youssefi & Saman Soleimanpour

  3. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Zahra Sabouri

  4. Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Javid Davoodi

  5. Chemistry Department, Payame Noor University, Mashhad, Iran

    Monireh Kazemi

  6. Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Kiarash Ghazvini

  7. Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Majid Darroudi

  8. Department of Medical Biotechnology & Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Majid Darroudi

Authors
  1. Fatemeh Adibian
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  2. Roya Saddat Ghaderi
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  3. Zahra Sabouri
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  8. Saman Soleimanpour
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Correspondence to Saman Soleimanpour or Majid Darroudi.

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Adibian, F., Ghaderi, R.S., Sabouri, Z. et al. Green synthesis of selenium nanoparticles using Rosmarinus officinalis and investigated their antimicrobial activity. Biometals 35, 147–158 (2022). https://doi.org/10.1007/s10534-021-00356-3

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