, Volume 31, Issue 2, pp 243–254 | Cite as

The metabolic effects of mercury during the biological cycle of vines (Vitis vinifera)

  • Adrián Spisso
  • Ernesto Verni
  • Keaton Nahan
  • Luis Martinez
  • Julio Landero
  • Pablo PachecoEmail author


Mercury (Hg) is a major environmental pollutant that can be disposed to the environment by human activities, reaching crops like vineyards during irrigation with contaminated waters. A 2-year study was performed to monitor Hg variations during reproductive and vegetative stages of vines after Hg supplementation. Variations were focused on total Hg concentration, the molecular weight of Hg fractions and Hg-proteins associations in roots, stems and leaves. Total Hg concentrations increased during reproductive stages and decreased during vegetative stages. Variations in length of these stages were observed, according to an extension of the vegetative period. Six months post Hg administration, in roots, stems and leaves, initial Hg proteic fractions of 200 kDa were catabolized to 66 kDa fractions according to a transition from reproductive to vegetative stages. However, 24 months after Hg supplementation, the 66 kDa Hg proteic fraction was continuously determined in a prolonged senescence. Accordingly, the identified proteins associated to Hg show catabolic functions such as endopeptidases, hydrolases, glucosidases and nucleosidases. Stress associated proteins, like peroxidase and chitinase were also found associated to Hg. During the reproductive periods of vines, Hg was associated to membrane proteins, such as ATPases and lipid transfer proteins, especially in roots where Hg is absorbed.


Mercury Proteins Vines Biological cycle 



We would like to thanks Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) for the scholarship (Spisso and Verni). We also would to thanks Agencia Nacional de Promoción Científica y Técnica (ANPCyT) and Universidad Nacional de San Luis (UNSL) for the funding. We are grateful to Agilent Technologies and CEM Corp. for the instrumentation loans.

Supplementary material

10534_2018_84_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)


  1. Chen YA et al (2012) Mercury-induced biochemical and proteomic changes in rice roots. Plant Physiol Biochem 55:23–32. CrossRefPubMedGoogle Scholar
  2. Chen YA et al (2014) Transcriptome profiling and physiological studies reveal a major role for aromatic amino acids in mercury stress tolerance in rice seedlings. PLoS ONE. Google Scholar
  3. Chopin EIB et al (2008) Factors affecting distribution and mobility of trace elements (Cu, Pb, Zn) in a perennial grapevine (Vitis vinifera L.) in the Champagne region of France. Environ Pollut 156:1092–1098. CrossRefPubMedGoogle Scholar
  4. Clemens S (2006) Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88:1707–1719. CrossRefPubMedGoogle Scholar
  5. Clemens S, Ma JF (2016) Toxic heavy metal and metalloid accumulation in crop plants and foods. Ann Rev Plant Biol. Google Scholar
  6. Cobbett C, Goldsbrough P (2002) Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Ann Rev Plant Biol 53:159–182. CrossRefGoogle Scholar
  7. Duarte B, Caetano M, Almeida PR, Vale C, Caçador I (2010) Accumulation and biological cycling of heavy metal in four salt marsh species, from Tagus estuary (Portugal). Environ Pollut 158:1661–1668. CrossRefPubMedGoogle Scholar
  8. Fabani MP, Toro ME, Vázquez F, Díaz MP, Wunderlin DA (2009) Differential absorption of metals from soil to diverse vine varieties from the valley of tulum (Argentina): consequences to evaluate wine provenance. J Agric Food Chem 57:7409–7416. CrossRefPubMedGoogle Scholar
  9. Gómez-Sagasti MT, Barrutia O, Ribas G, Garbisu C, Becerril JM (2016) Early transcriptomic response of: arabidopsis thaliana to polymetallic contamination: implications for the identification of potential biomarkers of metal exposure. Metallomics 8:518–531. CrossRefPubMedGoogle Scholar
  10. Greger M, Wang Y, Neuschütz C (2005) Absence of Hg transpiration by shoot after Hg uptake by roots of six terrestrial plant species. Environ Pollut 134:201–208. CrossRefPubMedGoogle Scholar
  11. Kang G et al (2015) Hg-responsive proteins identified in wheat seedlings using iTRAQ analysis and the role of ABA in Hg stress. J Proteom Res 14:249–267. CrossRefGoogle Scholar
  12. Keller M (2015) The science of grapevines: anatomy and physiology. Elsevier, AmsterdamGoogle Scholar
  13. Kembhavi AA, Buttle DJ, Knight CG, Barrett AJ (1993) The two cysteine endopeptidases of legume seeds: purification and characterization by use of specific fluorometric assays. Arch Biochem Biophys 303:208–213. CrossRefPubMedGoogle Scholar
  14. Komárek M, Čadková E, Chrastný V, Bordas F, Bollinger JC (2010) Contamination of vineyard soils with fungicides: a review of environmental and toxicological aspects. Environ Int 36:138–151. CrossRefPubMedGoogle Scholar
  15. Leita L, Mondini C, De Nobili M, Simoni A, Sequi P (1998) Heavy metal content in xylem sap (Vitis vinifera) from mining and smelting areas. Environ Monit Assess 50:189–200. CrossRefGoogle Scholar
  16. Li Y et al (2016a) Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots. Metallomics 8:663–671. CrossRefPubMedGoogle Scholar
  17. Li Y, Zhao J, Zhang B, Liu Y, Xu X, Li YF, Li B, Gao Y, Chai Z (2016b) The influence of iron plaque on the absorption, translocation and transformation of mercury in rice (Oryza sativa L.) seedlings exposed to different mercury species. Plant Soil 398(1–2):87–97. CrossRefGoogle Scholar
  18. Liu X, Wu H, Ji C, Wei L, Zhao J, Yu J (2013) An integrated proteomic and metabolomic study on the chronic effects of mercury in suaeda salsa under an environmentally relevant salinity. PLoS ONE. Google Scholar
  19. Miotto A et al (2014) Copper uptake, accumulation and physiological changes in adult grapevines in response to excess copper in soil. Plant Soil 374:593–610. CrossRefGoogle Scholar
  20. Mohapatra PK, Patro L, Raval MK, Ramaswamy NK, Biswal UC, Biswal B (2010) Senescence-induced loss in photosynthesis enhances cell wall β-glucosidase activity. Physiol Plant 138:346–355. CrossRefPubMedGoogle Scholar
  21. Möhlmann T, Bernard C, Hach S, Ekkehard Neuhaus H (2010) Nucleoside transport and associated metabolism. Plant Biol 12:26–34. CrossRefPubMedGoogle Scholar
  22. Mohr H, Lawlor G, Lawlor DW, Schopfer P (1995) Plant physiology. Springer, BerlinCrossRefGoogle Scholar
  23. Pacheco P, Hanley T, Landero Figueroa JA (2014) Identification of proteins involved in Hg-Se antagonism in water hyacinth (Eichhornia crassipes). Metallomics 6:560–571. CrossRefPubMedGoogle Scholar
  24. Pande M, Dubey VK, Yadav SC, Jagannadham MV (2006) A novel serine protease cryptolepain from Cryptolepis buchanani: purification and biochemical characterization. J Agric Food Chem 54:10141–10150. CrossRefPubMedGoogle Scholar
  25. Peralta-Videa JR, Lopez ML, Narayan M, Saupe G, Gardea-Torresdey J (2009) The biochemistry of environmental heavy metal uptake by plants: implications for the food chain. Int J Biochem Cell Biol 41:1665–1677. CrossRefPubMedGoogle Scholar
  26. Puzon JJM, Rivero GC, Serrano JE (2014) Antioxidant responses in the leaves of mercury-treated Eichhornia crassipes (Mart.) Solms. Environ Monit Assess 186:6889–6901. CrossRefPubMedGoogle Scholar
  27. Spisso A, Pacheco PH, Gómez FJV, Silva MF, Martinez LD (2013) Risk assessment on irrigation of vitis vinifera L. cv malbec with Hg contaminated waters. Environ Sci Technol 47:6606–6613. CrossRefPubMedGoogle Scholar
  28. Spisso AA, Cerutti S, Silva F, Pacheco PH, Martinez LD (2014) Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress. Biometals 27:591–599. CrossRefPubMedGoogle Scholar
  29. Todic S, Beslic Z, Lakic N, Tesic D (2006) Lead, mercury, and nickel in grapevine, Vitis vinifera L., in polluted and nonpolluted regions. Bull Environ Contam Toxicol 77:665–670. CrossRefPubMedGoogle Scholar
  30. Yathavakilla SKV, Caruso JA (2007) A study of Se-Hg antagonism in Glycine max (soybean) roots by size exclusion and reversed phase HPLC–ICPMS. Anal Bioanal Chem 389:715–723. CrossRefPubMedGoogle Scholar
  31. Zheng WJ, Chen XY, Peng L (1997) Accumulation and biological cycling of heavy metal elements in Rhizophora stylosa mangroves in Yingluo Bay, China. Mar Ecol Prog Ser 159:293–301CrossRefGoogle Scholar
  32. Zhou Q et al (2016) Comparative transcriptome analysis between low- and high-cadmium-accumulating genotypes of pakchoi (Brassica chinensis L.) in response to cadmium stress. Environ Sci Technol 50:6485–6494. CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de Química de San Luis (INQUISAL-CONICET)San LuisArgentina
  2. 2.Department of Chemistry, University of Cincinnati/Agilent Technologies, Metallomics Center of the AmericasUniversity of CincinnatiCincinnatiUSA

Personalised recommendations