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BioMetals

, Volume 31, Issue 2, pp 243–254 | Cite as

The metabolic effects of mercury during the biological cycle of vines (Vitis vinifera)

  • Adrián Spisso
  • Ernesto Verni
  • Keaton Nahan
  • Luis Martinez
  • Julio Landero
  • Pablo Pacheco
Article
  • 80 Downloads

Abstract

Mercury (Hg) is a major environmental pollutant that can be disposed to the environment by human activities, reaching crops like vineyards during irrigation with contaminated waters. A 2-year study was performed to monitor Hg variations during reproductive and vegetative stages of vines after Hg supplementation. Variations were focused on total Hg concentration, the molecular weight of Hg fractions and Hg-proteins associations in roots, stems and leaves. Total Hg concentrations increased during reproductive stages and decreased during vegetative stages. Variations in length of these stages were observed, according to an extension of the vegetative period. Six months post Hg administration, in roots, stems and leaves, initial Hg proteic fractions of 200 kDa were catabolized to 66 kDa fractions according to a transition from reproductive to vegetative stages. However, 24 months after Hg supplementation, the 66 kDa Hg proteic fraction was continuously determined in a prolonged senescence. Accordingly, the identified proteins associated to Hg show catabolic functions such as endopeptidases, hydrolases, glucosidases and nucleosidases. Stress associated proteins, like peroxidase and chitinase were also found associated to Hg. During the reproductive periods of vines, Hg was associated to membrane proteins, such as ATPases and lipid transfer proteins, especially in roots where Hg is absorbed.

Keywords

Mercury Proteins Vines Biological cycle 

Notes

Acknowledgements

We would like to thanks Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) for the scholarship (Spisso and Verni). We also would to thanks Agencia Nacional de Promoción Científica y Técnica (ANPCyT) and Universidad Nacional de San Luis (UNSL) for the funding. We are grateful to Agilent Technologies and CEM Corp. for the instrumentation loans.

Supplementary material

10534_2018_84_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de Química de San Luis (INQUISAL-CONICET)San LuisArgentina
  2. 2.Department of Chemistry, University of Cincinnati/Agilent Technologies, Metallomics Center of the AmericasUniversity of CincinnatiCincinnatiUSA

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