The cytotoxicity effects of a novel Cu complex on MCF-7 human breast cancerous cells
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A variety of biological activities, such as anti-microbial and anti-tumor properties was reported for 1,10-phenanthroline and its copper complexes. In this study, the anti-proliferative activity of a novel [Cu(L)(phen)] complex was investigated on MCF-7 breast cancer cells using MTT assay. Since chemotherapy is lake of ability to distinguish between normal cells from cancerous cells, therefore we also investigated the effect of [Cu(L)(phen)] complex on normal L929 cells. The results showed that following 24 and 48 h exposure of cells with [Cu(L)(phen)] complex, the IC50 values for MCF-7 were significantly lower than that recorded for L929 and normal cells were less sensitive than cancerous cells to the complex. Additionally, the [Cu(L)(phen)] complex displayed a time- and concentration-dependent cytotoxic response, with MCF-7 and L929 cells. Also flow cytometry findings suggest that [Cu(L)(phen)] complex is capable of decreasing cancer cell viability through apoptosis and did not efficiently activate the necrosis process.
KeywordsApoptosis Breast cancer [Cu(L)(phen)] complex Cytotoxicity MCF-7 cell line
Fetal bovine serum
Inhibiting cell growth by 50%
Phosphate buffered saline
Statistical package for the social sciences
World Health Organization
This article is an excerpt from Fatemeh Mohammadizadeh’s master’s thesis in Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences (RUMS), Rafsanjan, Iran. The authors would like to thank the Molecular Medicine Research Center (MMRC) in RUMS from of Iran for providing the necessary equipment for this work. This study was approved by the RUMS Ethical Committee by the Number of IR.RUMS.REC.1395.140.
This study was supported by the RUMS by the Grant Number 20.1326.
Compliance with ethical standards
Conflict of interest
The authors report no Conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
This article does not contain any studies with human participants.
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