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Decreased aluminium tolerance in the growth of Saccharomyces cerevisiae with SSO2 gene disruption

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Abstract

Aluminium ions inhibit growth of the budding yeast Saccharomyces cerevisiae. Disruption of the SSO2 gene increased the susceptibility to aluminium. Sso2p belongs to the soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) family. SSO2 has one paralogue, SSO1, which encodes Sso1p. The SNARE complex containing Sso1/2p plays a role in the recognition of plasma membrane targeted vesicle transport. The susceptibility to aluminium stress was not increased in the Δsso1 strain. The phenotype of aluminium ion influx between the wild-type and Δsso2 strains was not different, suggesting that Sso2p was involved in the elimination of cellular aluminium. However, the cellular lipid constitution of Δsso2 was richer in unsaturated fatty acids than the wild type, indicating that Sso2p is associated with lipid homeostasis of the plasma membrane. Aluminium treatment increased the production of reactive oxygen species (ROS) during proliferation. ROS production was increased in the Δsso2 strain after 3 h of aluminium treatment compared with the wild type. These results suggested that Sso2p plays a role in maintaining the lipid composition of the plasma membrane and the increase in unsaturated fatty acids amplified the production of ROS in the acute phase of aluminium stress. ROS derived from aluminium stress inhibited growth and resulted in the susceptibility of the Δsso2 strain.

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Acknowledgements

We are grateful to Dr. Kazuo Furihata for help in NMR analyses, Dr. Mayuki Tanaka for help in intracellular aluminium analyses, and Dr. Takehiko Sasaki, Dr. Tomohiro Furukawa and Dr. Shohei Sakuda for support of this work.

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Author notes

  1. Toshiyoshi Yamamoto and Daiki Yamamoto have contributed equally to this work.

Authors and Affiliations

  1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Toshiyoshi Yamamoto, Daiki Yamamoto, Keiji Rokugawa, Ko Yoshimura, Yuki Imura, Etsuro Yoshimura & Michio Suzuki

  2. The Open University of Japan, 2-11 Wakaba, Mishima-ku, Chiba, 261–8586, Japan

    Etsuro Yoshimura

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  1. Toshiyoshi Yamamoto
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  2. Daiki Yamamoto
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  5. Yuki Imura
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Correspondence to Etsuro Yoshimura or Michio Suzuki.

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10534_2017_69_MOESM1_ESM.eps

Fig S1 1H-1H DQF-COSY spectrum of yeast lipid extract. The diagram was obtained from the lipid extract of Δsso2 cultured in 100 μM of aluminium ions for 18 h. a: entire diagram, b:magnified diagram of (a) around 2.0 ppm. Numbers on axis indicates chemical shift (ppm). Supplementary material 1 (EPS 1984 kb)

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Yamamoto, T., Yamamoto, D., Rokugawa, K. et al. Decreased aluminium tolerance in the growth of Saccharomyces cerevisiae with SSO2 gene disruption. Biometals 31, 203–215 (2018). https://doi.org/10.1007/s10534-017-0069-z

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  • DOI: https://doi.org/10.1007/s10534-017-0069-z

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