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Four mononuclear platinum(II) complexes: synthesis, DNA/BSA binding, DNA cleavage and cytotoxicity

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Abstract

Four new platinum(II) complexes: PtII L1·H2O (C1, H2 L1 = C20H16N2O2), PtII L2Cl2 (C2, L2 = C22H16N2O2), PtII L3Cl2·H2O (C3, L3 = C20H16N2), PtII L4Cl2·0.4H2O (C4, L4 = C18H14N4) have been synthesized and characterized by using various physico-chemical techniques. The binding interaction of the four platinum(II) complexes C1C4 with calf thymus (CT)-DNA has been investigated by UV–Vis and fluorescence emission spectrometry. The apparent binding constant (K app) values follow the order: C3 > C1 > C2 > C4. In addition, fluorescence spectrometry of bovine serum albumin (BSA) with the four platinum(II) complexes C1C4 showed that the quenching mechanism might be a static quenching procedure. For C1C4, the number of binding sites was about one for BSA and the binding constants follow the order: C3 (7.08 × 105M−1) > C1 (2.82 × 105M−1) > C2 (0.85 × 105M−1) > C4 (0.15 × 105M−1). With the single condition change such as absence of an external agent, the DNA cleavage abilities of C3 exhibit remarkable changes. In addition, the cytotoxicity of C3 in vitro on tumor cells lines (MCF-7, HepG2 and HT29) were examined by MTT and showed better antitumor effects on the tested cells.

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Abbreviations

DNA:

Deoxyribonucleic acid

BSA:

Bovine serum albumin

EB:

Ethidium bromide

MTT:

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide

H 2 L1 :

C20H16N2O2

L2 :

C22H16N2O2

L3 :

C20H16N2

L4 :

C18H14N4

C1 :

C20H14N2O2Pt·H2O

C2 :

C22H16Cl2N2O2Pt

C3 :

C20H16Cl2N2Pt·H2O

C4 :

C18H14Cl2N4Pt·0.4H2O

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Acknowledgements

This work were financially supported by the National Natural Science Foundation for Young Scientists of China (21301150, 21571154), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (13KJB150037, 14KJB150027), the Post-Doctoral Foundation of Jiangsu Provincial (1501032B), the six Taleng Peak Project in Jiangsu Province (SWYY-063), the practice inovation trainng program projects for the Jiangsu College students (201310324014Z, 201410324053), the Natural Science Foundation of Yancheng Teachers University (10YCKL017,14YCKL006) and sponsored by Qing Lan Project of Jiangsu Provices.

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Correspondence to Qingming Wang.

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Wang, Q., Yang, L., Wu, J. et al. Four mononuclear platinum(II) complexes: synthesis, DNA/BSA binding, DNA cleavage and cytotoxicity. Biometals 30, 17–26 (2017). https://doi.org/10.1007/s10534-016-9984-7

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