, Volume 28, Issue 5, pp 803–816 | Cite as

Cadmium stress antioxidant responses and root-to-shoot communication in grafted tomato plants

  • Priscila Lupino Gratão
  • Carolina Cristina Monteiro
  • Tiago Tezotto
  • Rogério Falleiros Carvalho
  • Letícia Rodrigues Alves
  • Leila Priscila Peters
  • Ricardo Antunes AzevedoEmail author


Many aspects related to ROS modulation of signaling networks and biological processes that control stress responses still remain unanswered. For this purpose, the grafting technique may be a powerful tool to investigate stress signaling and specific responses between plant organs during stress. In order to gain new insights on the modulation of antioxidant stress responses mechanisms, gas-exchange measurements, lipid peroxidation, H2O2 content, proline, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) and guaiacol peroxidase (GPOX) were analyzed in Micro-Tom grafted plants submitted to cadmium (Cd). The results observed revealed that higher amounts of Cd accumulated mainly in the roots and rootstocks when compared to leaves and scions. Macronutrients uptake (Ca, S, P and Mg) decreased in non-grafted plants, but differed among plant parts in all grafted plants. The results showed that the accumulation of proline observed in scions of grafted plants could be associated to the lower MDA contents in the scions of grafted plants. In the presence of Cd, non-grafted plants displayed increased CAT, GR, GPOX and APX activities for both tissues, whilst grafted plants revealed distinct trends that clearly indicate signaling responses from the rootstocks, allowing sufficient time to activate defense mechanisms in shoot. The information available concerning plants subjected to grafting can provide a better understanding of the mechanisms of Cd detoxification involving root-to-shoot signaling, opening new possibilities on strategies which can be used to manipulate heavy metal tolerance, since antioxidant systems are directly involved in such mechanism.


Cadmium Micro-Tom Grafting Signaling Oxidative stress 



This work was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP - Grant no.09/54676-0). We thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) (R.A.A. and L.P.P.), and FAPESP (P.L.G., C.C.M. and T.T.) for the fellowships and scholarships granted.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Priscila Lupino Gratão
    • 1
  • Carolina Cristina Monteiro
    • 1
  • Tiago Tezotto
    • 2
  • Rogério Falleiros Carvalho
    • 1
  • Letícia Rodrigues Alves
    • 1
  • Leila Priscila Peters
    • 3
  • Ricardo Antunes Azevedo
    • 3
    Email author
  1. 1.Depto. de Biologia Aplicada à Agropecuária (DBAA)UNESP – Univ. Estadual PaulistaJaboticabalBrazil
  2. 2.Centro Universitário da Fundação de Ensino Octávio Bastos, UnifeobSão João da Boa VistaBrazil
  3. 3.Depto. de Genética, Escola Superior de Agricultura Luiz de Queiroz-ESALQUniversidade de São Paulo-USPPiracicabaBrazil

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