BioMetals

, Volume 28, Issue 1, pp 11–20 | Cite as

Estimation of trace metal elements in oral mucosa specimens by using SR-XRF, PIXE, and XAFS

  • Tomoko Sugiyama
  • Motohiro Uo
  • Takahiro Wada
  • Daisuke Omagari
  • Kazuo Komiyama
  • Tadahide Noguchi
  • Yoshinori Jinbu
  • Mikio Kusama
Article

Abstract

The effects of dissolved elements from metal dental restorations are a major concern in lesions of the oral mucosa, and the evaluation of accumulated metal elements, especially their distribution and chemical state, is essential for determining the precise effects of trace metals. In this study, X-ray fluorescence with synchrotron radiation (SR-XRF) and particle-induced X-ray emission (PIXE) were applied for distribution analysis of the trace metal elements contained in the oral mucosa, and the chemical states of the elements were estimated using X-ray absorption fine structure (XAFS) analysis. Appropriate combination of these analysis techniques, particularly SR-XRF and PIXE, to visualize the distributions of the elements in the oral mucosa allowed for the observation and evaluation of accumulated metal ions and debris. Importantly, the analyses in this study could be carried out using conventional histopathological specimens without damaging the specimens. Therefore, this method would be applicable for the detection of accumulated trace metal elements in biopsy specimens from the oral mucosa.

Keywords

Synchrotron radiation X-ray fluorescence analysis Particle-induced X-ray emission analysis X-ray absorption fine structure analysis Trace elemental analysis Histopathological specimen 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Tomoko Sugiyama
    • 1
  • Motohiro Uo
    • 2
  • Takahiro Wada
    • 2
  • Daisuke Omagari
    • 3
  • Kazuo Komiyama
    • 3
  • Tadahide Noguchi
    • 1
  • Yoshinori Jinbu
    • 1
  • Mikio Kusama
    • 1
  1. 1.Department of Dentistry, Oral and Maxillofacial SurgeryJichi Medical UniversityShimotsukeJapan
  2. 2.Advanced Biomaterials Department, Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityTokyoJapan
  3. 3.Department of PathologyNihon University School of DentistryTokyoJapan

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