Abstract
Fluorescent pseudomonads produce a characteristic fluorescent pigment, pyoverdines as their primary siderophore for iron acquisition under iron-limiting conditions. Here, we report the identification of a random transposon mutant IST3 of Pseudomonas putida S11 showing tolerance to iron starvation stress condition and increased pyoverdine production. The insertion of the Tn5 transposon was found to be in pstS gene of pstSR operon encoding sensor histidine kinase protein of the two-component signal transduction system. A pyoverdine negative derivative of IST3 mutant constructed was sensitive to iron stress condition. It indicated that increased survival of IST3 under iron-limiting condition was due to higher pyoverdine production. The iron starvation tolerant mutant (IST3) exhibited enhanced pyoverdine-mediated iron uptake in minimal medium which significantly improved its biofilm formation, seed adhesion and competitive root colonization.
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Acknowledgments
The work was financially supported by the Indian Council for Agricultural Research (NBAIM/AMAAS/2007-2012/MG (5)/PG/BG/3), India. The authors thank H.P.Schweizer for plasmids pUCP24 and pEX18Tc and F.Wisniewski-Dye for E. coli S17-1 λ-pir strain. Support facilities from Centre for Advanced studies in Functional Genomics, Centre for Excellence in Genomic Sciences, UGC-Networking Resource Centre in Biological Sciences and DBT-MKU Interdisciplinary Life Science Programme for Advanced Research and Education are gratefully acknowledged. PP gratefully acknowledges the valuable technical assistance and discussions from M. Jeya and S. Thiagarajan.
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Ponraj, P., Shankar, M., Ilakkiam, D. et al. Influence of siderophore pyoverdine synthesis and iron-uptake on abiotic and biotic surface colonization of Pseudomonas putida S11. Biometals 25, 1113–1128 (2012). https://doi.org/10.1007/s10534-012-9574-2
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DOI: https://doi.org/10.1007/s10534-012-9574-2