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Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons

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Abstract

Selenium (Se) and Selenoprotein W (SelW) plays a pivotal role in the brain development, function, and degeneration and that SelW expression in the brain may be affected by Se. However, the mechanism which Se regulates the SelW gene expression in neurons remains to be unclear. To investigate the effects of the SelW gene expression and mRNA stability induced by Se, primary cultured chicken embryos neurons derived from 8-day-old chick embryo cerebral hemispheres were treated with 10−9–10−5 mol/l Se as selenite for 3, 6, 12, 24 or 48 h, respectively. The morphology and viability of Neurons was detected. The SelW mRNA expression level and mRNA half-life was examined in Se-treated neurons. The relative low concentrations of Se enhanced the neurite outgrowth, increased the SelW mRNA levels and elevated the mRNA half-life of chick embryo neurons. In contrast, the high concentrations of Se presented neurotoxic to neurons, decreased the SelW mRNA levels and reduced the mRNA half-life of neuronal cells. These results suggest that the alteration of post-transcriptional stabilization of SelW mRNA is an important mechanism of Se-induced the elevation or reduction of the SelW expression level in chick embryo neurons.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 30871902) and the Science Foundation of the Education Department of Heilongjiang Province (Grant No. 11551030).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jin-Long Li, Shu Li, Xue-Jiao Gao & Shi-Wen Xu

  2. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510640, People’s Republic of China

    Jin-Long Li & Zhao-Xin Tang

  3. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People’s Republic of China

    Hui-Xin Li

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  1. Jin-Long Li
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  2. Hui-Xin Li
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  5. Shi-Wen Xu
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Correspondence to Shi-Wen Xu or Zhao-Xin Tang.

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Fig. S1

Effects of Se and incubation period on the expression of GADPH mRNA in chicken embryo neurons. GADPH mRNA expression level in neurons was measured by quantitative real-time RT-PCR. Bars represent mean ± standard deviation of triplicate cultures. (PPT 165 kb)

Fig. S2

Effects of Se on the half-life of SelW mRNA in chicken embryo neurons. The chicken embryo neuron monolayers were treated with 0 mol/l, ActD, ActD + 10−8 mol/l Se, ActD + 10−7 mol/l Se, ActD + 10−6 mol/l Se or ActD + 10−5 mol/l Se for 0 h, 3 h, 6 h, 9 h, 12 h, 24 h or 48 h, respectively. SelW mRNA expression level in chicken embryo neurons was measured by quantitative real-time RT-PCR. The RNA half-life was extrapolated from the SelW mRNA decay curve as the time point after 5μg/mL ActD treatment. (PPT 367 kb)

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Li, JL., Li, HX., Li, S. et al. Effects of Selenoprotein W gene expression by selenium involves regulation of mRNA stability in chicken embryos neurons. Biometals 25, 459–468 (2012). https://doi.org/10.1007/s10534-012-9517-y

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