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Pseudomonas siderophores in the sputum of patients with cystic fibrosis

BioMetals volume 24, pages 1059–1067 (2011)Cite this article

Abstract

The lungs of patients with cystic fibrosis become chronically infected with the bacterium Pseudomonas aeruginosa, which heralds progressive lung damage and a decline in health. Iron is a crucial micronutrient for bacteria and its acquisition is a key factor in infection. P. aeruginosa can acquire this element by secreting pyoverdine and pyochelin, iron-chelating compounds (siderophores) that scavenge iron and deliver it to the bacteria. Siderophore-mediated iron uptake is generally considered a key factor in the ability of P. aeruginosa to cause infection. We have investigated the amounts of pyoverdine in 148 sputum samples from 36 cystic fibrosis patients (30 infected with P. aeruginosa and 6 as negative controls). Pyoverdine was present in 93 samples in concentrations between 0.30 and 51 μM (median 4.6 μM) and there was a strong association between the amount of pyoverdine and the number of P. aeruginosa present. However, pyoverdine was not present, or below the limits of detection (~0.3 μM), in 21 sputum samples that contained P. aeruginosa. Pyochelin was also absent, or below the limits of detection (~1 μM), in samples from P. aeruginosa-infected patients with little or no detectable pyoverdine. Our data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. aeruginosa iron-uptake systems must be active in some patients to satisfy the bacterial need for iron.

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Abbreviations

CF:

Cystic fibrosis

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Acknowledgments

This work was supported by Research Grant 352611 from The Australian National Health Research Council. We gratefully acknowledge the generous gift of pyochelin from Gaetan Mislin and Isabelle Schalk.

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Author notes

  1. David W. Reid

    Present address: Department of Thoracic Medicine, The Prince Charles Hospital, Chermside, Brisbane, QLD, 4032, Australia

Affiliations

  1. Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand

    Lois W. Martin & Iain L. Lamont

  2. Department of Preventive and Social Medicine, University of Otago, P.O. Box 56, Dunedin, New Zealand

    Katrina J. Sharples

  3. Menzies Research Institute and University of Tasmania Medical School, 43 Collins Street, Hobart, TAS, 7001, Australia

    David W. Reid

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  1. Lois W. Martin
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  2. David W. Reid
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  4. Iain L. Lamont
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Correspondence to Iain L. Lamont.

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Martin, L.W., Reid, D.W., Sharples, K.J. et al. Pseudomonas siderophores in the sputum of patients with cystic fibrosis. Biometals 24, 1059–1067 (2011). https://doi.org/10.1007/s10534-011-9464-z

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  • DOI: https://doi.org/10.1007/s10534-011-9464-z

Keywords

  • Pyoverdine
  • Pyochelin
  • Iron and infection
  • Pseudomonas aeruginosa
  • Cystic fibrosis