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Metallic ion content and damage to the DNA in oral mucosa cells of children with fixed orthodontic appliances

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A Correction to this article was published on 03 May 2018

Abstract

Although the metal devices used in orthodontic treatments are manufactured highly resistance to corrosion, they may still suffer some localized corrosion resulting from the oral cavity conditions. The corrosion causes the release of metals from the alloys used for their manufacture. In this report, we evaluated the in vivo metal ions release of three alloys (stainless steel, titanium and nickel-free) usually used in the orthodontics treatments and its genotoxicity. We applied to 15 patients, between 12 and 16 years, 4 tubes and 20 brackets. Samples from oral mucosa were taken before the treatment and 30 days later. The concentration of the titanium, chromium, manganese, cobalt, nickel, molybdenum and iron were detected using inductively coupled plasma mass spectrometry (ICP-MS). The genotoxicity was measured with a comet assay (Olive moment). The oral mucosa cells in contact with the stainless steel alloy displayed the greatest titanium and manganese concentrations and those in contact with the nickel-free alloy presented the greatest concentration of chromium and iron. Both alloys, stainless steel and nickel-free, induced a higher DNA damage in the oral mucosa cells than the titanium alloy, in which the Olive moment was similar to controls. Based on the results of our study, we can conclude that titanium brackets and tubes are the most biocompatible of the three alloys.

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Acknowledgments

This work was financially supported by Seneca Foundation of the Region of Murcia (Spain) under a grant (n° 100260).

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Authors and Affiliations

  1. Integral Pediatric Dentistry Teaching Unit, Faculty of Medicine, Dental Clinic, University of Murcia, Morales Meseguer Hospital, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain

    Esther Fernández-Miñano & Antonio J. Ortiz

  2. Department of Physiology, Faculty of Medicine, University of Murcia, 30100, Murcia, Spain

    Clara Ortiz

  3. Docent Unit of Orthodontics, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain

    Ascensión Vicente

  4. General Dentistry, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain

    José L. Calvo

Authors
  1. Esther Fernández-Miñano
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  2. Clara Ortiz
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  3. Ascensión Vicente
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  4. José L. Calvo
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  5. Antonio J. Ortiz
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Correspondence to Antonio J. Ortiz.

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Fernández-Miñano, E., Ortiz, C., Vicente, A. et al. Metallic ion content and damage to the DNA in oral mucosa cells of children with fixed orthodontic appliances. Biometals 24, 935–941 (2011). https://doi.org/10.1007/s10534-011-9448-z

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  • DOI: https://doi.org/10.1007/s10534-011-9448-z

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