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Spectroscopic studies of amphotericin B–Cu2+ complexes

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Abstract

The aim of this research is to investigate amphotericin B (AmB)–Cu2+ complexes in aqueous solution at different pH values. Electronic absorption, circular dichroism (CD), Raman and FTIR spectroscopies were used in this study. We found that different concentrations of AmB and Cu2+ ions in solution leads to formation of complexes with stoichiometry of 2:1 and 1:1. The formation of AmB–Cu2+ complexes at physiological pH values is accompanied by changes of the molecular organization of AmB especially disaggregation. These observed effects might be significant from a medical point of view.

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Acknowledgments

This research was financed by the Ministry of Education and Science of Poland from the budget funds for science in the years 2008–2011 within the research project N N401 015035. Authors thanks to MSc Viktoria Babenko from Department of Chemistry University of Warsaw Poland for assistance with CD measurements.

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Authors and Affiliations

  1. Department of Biophysics, University of Life Sciences in Lublin, Akademicka 13, 20-950, Lublin, Poland

    Mariusz Gagoś & Grzegorz Czernel

  2. Department of Chemistry, University of Life Sciences in Lublin, Akademicka 15, 20-950, Lublin, Poland

    Daniel M. Kamiński

  3. Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612, Lublin, Poland

    Krzysztof Kostro

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  1. Mariusz Gagoś
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  2. Grzegorz Czernel
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  3. Daniel M. Kamiński
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  4. Krzysztof Kostro
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Correspondence to Mariusz Gagoś.

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Gagoś, M., Czernel, G., Kamiński, D.M. et al. Spectroscopic studies of amphotericin B–Cu2+ complexes. Biometals 24, 915–922 (2011). https://doi.org/10.1007/s10534-011-9445-2

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  • DOI: https://doi.org/10.1007/s10534-011-9445-2

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