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Effect of Ni2+ and Cd2+ ions on thermally induced conformational transitions in poly(dA)-poly(dT) system

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Abstract

Effects of Ni2+ and Cd2+ ions on thermally induced conformational transitions in the poly(dA)·poly(dT) polynucleotide duplex and poly(dA)·2poly(dT) triplex under near physiological ionic conditions were studied by measurement of UV absorption melting curves and static light scattering intensity. The diagrams of conformational transitions in poly(dA)-poly(dT)-Me2+ systems were plotted. An aggregation in these polynucleotide systems arises at certain values of the metal ions concentration and the temperature after the polymer dissociation into single strands. The phenomenon is conditioned by the aggregation of poly(dA) via the interstrand cross-linking by the dication bridges. Unlike Ni2+, Cd2+ induces formation of very stable aggregates which did not disintegrate even upon cooling up to room temperature.

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Acknowledgments

This work was supported by Government of Ukraine and US Civilian Research and Development Foundation for the Independent States of the Former Soviet Union (CRDF grant UN1-435).

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Authors and Affiliations

  1. Department of Molecular Biophysics, B.Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine, 47 Lenin ave, 61103, Kharkov, Ukraine

    Victor N. Zozulya, Olga A. Ryazanova & Yurii P. Blagoi

  2. Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody sq, 61077, Kharkov, Ukraine

    Nataliya N. Zhigalova

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  1. Victor N. Zozulya
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  2. Olga A. Ryazanova
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  3. Nataliya N. Zhigalova
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  4. Yurii P. Blagoi
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Correspondence to Victor N. Zozulya.

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Zozulya, V.N., Ryazanova, O.A., Zhigalova, N.N. et al. Effect of Ni2+ and Cd2+ ions on thermally induced conformational transitions in poly(dA)-poly(dT) system. Biometals 23, 1191–1201 (2010). https://doi.org/10.1007/s10534-010-9369-2

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