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Comparative effect of water and food-chain mediated cadmium exposure in rats

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Abstract

This study sets out to compare the absorption and toxicity of Cadmium (Cd) administered via the food-chain and inorganic Cd administered in drinking water after 1 and 3 months exposure using rats as animal model. The food-chain was mimicked by exposing rats to diet containing Cd pre-exposed fish. The uptake of Cd by the rats after both mode of exposure was calculated by summing up the Cd burden in the liver and kidneys and was expressed in terms of % intake. The toxicity of Cd was assessed by monitoring biochemical indices of liver function in the plasma and liver. Regardless of the mode of exposure of the rats, the Cd load in the liver and kidney was significantly (P < 0.05) higher than the respective controls with the kidney having a significantly higher load than the liver after both periods of exposure. However irrespective of the mode of exposure, more Cd was accumulated in the liver and kidney of the 3 months exposed rats relative to those exposed for 1 month. The uptake of Cd by rats exposed to Cd via the food-chain for 1 and 3 months was significantly (P < 0.05) lower when compared to the corresponding water mediated Cd exposed rats, except for the liver after 3 months of exposure. The liver l-ALT activity of rats administered inorganic Cd in drinking water for 1 and 3 months was significantly (P < 0.05) lower as compared to controls. Parallel analysis of the plasma showed no significant (P > 0.05) difference in l-ALT activity between both groups after the same periods of exposure. The l-AST activity in the plasma of rats similarly exposed to Cd for 1 and 3 months was significantly (P < 0.05) higher as compared to controls with a corresponding reduction in the liver. Conversely no significant (P > 0.05) change was observed in plasma and liver l-ALT and l-AST activities after food-chain mediated exposure to Cd for 1 and 3 months in relation to their respective controls. These findings indicate that Cd incorporated in fish is more easily bioavailable, but less toxic relative to inorganic Cd salts at the end of 3 months of exposure in rats.

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References

  • Andersen O, Nielsen JB, Nordberg GF (1992) Factors affecting the intestinal uptake of cadmium from the diet. In: Nordberg GF, Herber RFM, Alessio L (eds) Cadmium and the environment. IARC, Lyon, pp 173–187

    Google Scholar 

  • Asagba SO, Eriyamremu GE (2007) Oral cadmium exposure alters haematological and liver function parameters of rats fed a Nigerian-like diet. J Nutr Environ Med 16(1–3):267–274

    Article  CAS  Google Scholar 

  • Asagba SO, Eriyamremu GE, Adaikpoh MA, Ezeoma A (2004) Levels of lipid peroxidation, superoxide dismutase and Na+/K+-ATPase in some tissues of rats exposed to a Nigerian-like diet and cadmium. Biol Trace Elem Res 100(1):075–086

    Article  CAS  Google Scholar 

  • Asagba SO, Eriyamremu GE, Igberaese ME (2007) Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (Clarias gariepinus). Fish Physiol Biochem 34:61–69

    Article  PubMed  Google Scholar 

  • Bagchi D, Bagchi M, Hassoun EA, Stohs SJ (1996) Cadmium induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion and hepatic lipid peroxidation in Sprage-Dawley rats. Biol Trace Elem Res 52(2):143–154

    Article  CAS  PubMed  Google Scholar 

  • Bagchi D, Joshi SS, Bagchi M et al (2000) Cadmium- and chromium-induced oxidative stress, DNA damage and apoptotic cell death in cultured human chronic myelogenous leukemic K526 cells, promyelocytic leukemic HL-60 cells and normal human peripheral blood mononuclear cells. J Biochem Mol Toxicol 14:33–41

    Article  CAS  PubMed  Google Scholar 

  • Borges LP, Brandäo R, Grodoi B, Norqueira CW, Zeni G (2008) Oral administration of diphenyl diselenide protects against Cd induced liver damage in rats. Chem Biol Interact 171(1):15–25

    Article  CAS  PubMed  Google Scholar 

  • Brzόska MM, Moniuszko-Jakoniuk J, Jurczuk M, Galazyn-Sidorczuk M, Rogalska J (2000) Effect of short-term ethanol administration on cadmium retention and bioelement metabolism in rats continuously exposed to cadmium. Alcohol Alcohol 35(5):439–445

    Google Scholar 

  • Brzόska MM, Moniuszko-Jakoniuk J, Jurczuk M, Galazyn-Sidorczuk M (2002) Cadmium turnover and changes of zinc and copper body status of rats continuously exposed to cadmium and ethanol. Alcohol Alcohol 37(3):213–221

    Google Scholar 

  • Chang X, Jin T, Chen L, Nordberg M, Lei L (2009) Metallothionein 1 isoform mRNA expression in peripheral lymphocytes as a biomarker for occupational cadmium exposure. Exp Biol Med (Maywood) 234(6):666–672

    Article  CAS  Google Scholar 

  • Chater S, Douki T, Favier A, Sakly M, Abdelmelek H (2009) Changes in antioxidant status and biochemical parameters after orally cadmium administration in female rats. Acta Biol Hung 60(1):79–88

    Article  CAS  PubMed  Google Scholar 

  • Cherian MG, Goyer RA, Valberg LS (1978) Gastrointestinal absorption and organ distribution of oral cadmium chloride and cadmium-metallothionein in mice. J Toxicol Environ Health 4:861–868

    Article  CAS  PubMed  Google Scholar 

  • Chmielnicka J, Sowa B (1996) Cadmium interaction with essential metals (Zn, Cu, Fe) metabolism, metallothionein and ceruloplasmin in pregnant rats and fetuses. Ecotoxicol Environ Saf 35:277–281

    Article  CAS  PubMed  Google Scholar 

  • Crowe A, Morgan HE (1997) Effect of dietary cadmium on iron metabolism in growing rats. Toxicol Appl Pharmacol 145:136–146

    Article  CAS  PubMed  Google Scholar 

  • Elsenhans B, Strugala GJ, Schäfer SG (1997) Small-intestinal absorption of cadmium and the significance of mucosal metallothionein. Hum Exp Toxicol 16(8):429–434

    Article  CAS  PubMed  Google Scholar 

  • Gorski JR, Muzi G, Weber LW, Feriera DW, Latropoulos MJ, Rozman K (1988) Elevated plasma corticosterone levels and histopathology of the adrenals and thymuses in 2,3,7,8 tetrachlorodibenzo-P-dioxin treated rats. Toxicology 53:19–32

    Article  CAS  PubMed  Google Scholar 

  • Groten JP, Sinkeldam EJ, Luken JB, van Bladeren PJ (1990) Comparison of the toxicity of inorganic and liver incorporated cadmium: a 4-wk feeding study in rats. Food Chem Toxicol 28(6):435–441

    Article  CAS  PubMed  Google Scholar 

  • Groten JP, Koeman JH, Van Nesselrooij JHJ, Luten JB, Fentener Van Vlissingen JM, Stenhuis WS, Van Bladeren PJ (1994) Comparison of renal toxicity after long-term oral administration of cadmium chloride and cadmium-metallothionein in rats. Toxicol Sci 23(4):544–552

    Article  CAS  Google Scholar 

  • Guilhermino L, Soares AMVM, Carvalho AP, Lopes MC (1998) Effects of cadmium and parathion exposure on hematology and blood biochemistry of adult male rats. Bull Environ Contam Toxicol 60:52–59

    Article  CAS  PubMed  Google Scholar 

  • Haouem S, Hmad N, Najjar MF, El Hani A, Sakly R (2007) Accumulation of cadmium and its effects on liver and kidney functions in rats given diet containing cadmium polluted radish bulb. Exp Toxicol Pathol 59(1):77–80

    Article  CAS  PubMed  Google Scholar 

  • HO W (1992) Environmental health criteria, 134. World Health Organisation, Cadmium, Geneva

    Google Scholar 

  • Horiguchi H, Sato M, Konno N, Fukushima M (1996) Long-term cadmium-exposure induces anaemia in rats through hypoinduction of erythropoietin in the kidney. Arch Toxicol 71:11–19

    Article  CAS  PubMed  Google Scholar 

  • Kikuchi Y, Nomiyama T, Kumagai N et al (2003) Uptake of cadmium in meals from the digestive tract of young non-smoking Japanese female volunteers. J Occup Health 45:43–57

    Article  CAS  PubMed  Google Scholar 

  • Klaassen CD, Liu J, Diwan BA (2009) Metallothionein protection of cadmium toxicity. Toxicol Appl Pharmacol 238(3):215–220

    Article  CAS  PubMed  Google Scholar 

  • Kuester RK, Waalkes MP, Goering PL, Fishers BL, Micuskey RS, Sipes IG (2002) Differential hepatotoxicity induced by cadmium in Fisher 344 and Sprague-Dawley rats. Toxicol Sci 65:151–159

    Article  CAS  PubMed  Google Scholar 

  • Lind Y, Wicklund GA, Engman J, Jorhem L (1995) Bioavailability of cadmium from crab hepatopancreas and mushroom in relation to inorganic cadmium; a 9-week feeding study in mice. Food Chem Toxicol 38(8):667–673

    Google Scholar 

  • Lind Y, Engman J, Jorhem L, Glynn AW (1998) Accumulation of cadmium from wheat bran, sugar-beat fibre, carrots and cadmium chloride in the liver and kidneys of mice. Br J Nutr 80(2):205–211

    CAS  PubMed  Google Scholar 

  • Manuel Y, Thomas Y, Pellegrini O (1992) Metallothionein and tissue damage. In: Nordberg GF et al (eds) Cadmium and the environment. International Agency for Research on Cancer, Lyon, pp 231–237

    Google Scholar 

  • Min KS, Nakatsubo T, Kawamura S, Fujita Y, Onosaka S, Tanaka K (1992) Effects of mucosal metallothionein in small intestine on tissue distribution of cadmium after oral administration of cadmium compounds. Toxicol Appl Pharmacol 113(2):306–310

    Article  CAS  PubMed  Google Scholar 

  • Muller L, Abel J, Ohnesorge FK (1986) Absorption and distribution of cadmium (Cd), copper and zinc following oral subchronic low level administration to rats after different binding forms of cadmium (Cd-acetate, Cd-metallothionein, Cd-glutathione). Toxicology 39:187–195

    Article  CAS  PubMed  Google Scholar 

  • National Research Council (1996) Guide for the care and use of laboratory animals 1996. National Academy Press, Washington, DC

    Google Scholar 

  • Ohta H, Cherian MG (1991) Gastrointestinal absorption of cadmium and metallothionein. Toxicol Appl Pharmacol 107(1):63–72.

    Article  CAS  PubMed  Google Scholar 

  • Ohta H, Seki Y, Imamiva S (1993) Possible role of metallothionein on the gastrointestinal absorption and distribution of cadmium. Kitasato Arch Exp Med 65:137–145

    PubMed  Google Scholar 

  • Oshi S, Nakagawa J, Ando M (2000) Effects of cadmium administration on the endogenous metal balance in rats. Biol Trace Elem Res 76(3):257–278

    Google Scholar 

  • Patra RC, Swarup D, Senapati SK (1999) Effects of cadmium on lipid peroxides and superoxide dismutase in hepatic, renal and testicular tissue of rats. Vet Hum Toxicol 41:65–67

    CAS  PubMed  Google Scholar 

  • Reeves PG, Vanderpool RA (1998) Organ content and fecal excretion of cadmium in male and female rats consuming variable amounts of naturally occurring cadmium in confectionery sunflower kernels (Helianthus annuus L.). J Nutr Biochem 9(11):636–644

    Article  CAS  Google Scholar 

  • Reeves PG, Chaney RL, Simmons RW, Cherian MG (2005) Metallothionein induction is not involved in cadmium accumulation in the duodenum of mice and rats fed diets containing high-cadmium rice or sunflower kernels and a marginal supply of zinc, iron and calcium. J Nutr 135:99–108

    CAS  PubMed  Google Scholar 

  • Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am J Clin Pathol 28:56–58

    CAS  PubMed  Google Scholar 

  • Roqalska J, Brzóska MM, Roszczenko A, Moniuszko-Jakonuik J (2009) Enhanced zinc consumption prevents cadmium induced alterations in lipid metabolism in male rats. Chem Biol Interact 177(2):142–152

    Article  Google Scholar 

  • Sabbioni E, Marafante E, Amantini L, Ubertalli L, Petra R (1978) Cadmium toxicity studies under long term low-level exposure (LLE) conditions. 1. Metabolic patterns in rats exposed to present environmental dietary level of Cd for two years. Sci Total Environ 10(2):131–161

    CAS  PubMed  Google Scholar 

  • Sarkar S, Yadar P, Trivedi R, Bansal AK, Bhatnagar D (1995) Cadmium-induced lipid peroxidation and the status of the antioxidant system in rat tissues. J Trace Elem Med Biol 9(3):144–149

    CAS  PubMed  Google Scholar 

  • Shukla V, Dhankhar M, Prakash J, Sastry KV (2007) Bioaccumulation of Zn, Cu, and Cd in Channa punctatus. J Environ Biol 28(2):395–397

    CAS  PubMed  Google Scholar 

  • Siewicki TC, Van Dolah FM, Logan JS (1987) Cadmium retention in mice fed radiolabeled rat liver, eastern and pacific oysters, and hardshell and softshell clams. Nutr Reports Inter. 36:1291–1304

    CAS  Google Scholar 

  • Sugawara N, Sugawara C (1991) Gastrointestinal absorption of Cd-metallothionein and cadmium chloride in mice. Arch Toxicol 65:689–692

    Article  CAS  PubMed  Google Scholar 

  • Sullivan MF, Hardy JT, Miller BM, Buschbom RL, Siewicki TC (1984) Absorption and distribution of cadmium in mice fed diets containing either inorganic or oyster-incorporated cadmium. Toxicol Appl Pharmacol 72:210–217

    Article  CAS  PubMed  Google Scholar 

  • Timbrell J (2000) Principles of biochemical toxicology, 3rd edn edn. Taylor & Francis, London

    Google Scholar 

  • Vahter M, Berglund M, Nermell B, Akesson A (1996) Bioavailability of cadmium from shell fish and mixed diet in women. Toxicol Appl Pharmacol 136(2):332–341

    Article  CAS  PubMed  Google Scholar 

  • Zikic R, Stajn A, Saicic Z, Spasic M, Ziemnicki K, Petrovic V (1996) The activities of superoxide dismutase, catalase and ascorbic acid content in the liver of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiol Res 45(6):479–481

    CAS  PubMed  Google Scholar 

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Acknowledgements

Many thanks to Prof F.O. Obi of the Department of Biochemistry, University of Benin, Benin-City, Nigeria, for his guidance during the course of this study.

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  1. Department of Biochemistry, Delta State University, Abraka, Nigeria

    Samuel Ogheneovo Asagba

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Correspondence to Samuel Ogheneovo Asagba.

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Asagba, S.O. Comparative effect of water and food-chain mediated cadmium exposure in rats. Biometals 23, 961–971 (2010). https://doi.org/10.1007/s10534-010-9313-5

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