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Electrochemical and spectroscopic evidences of the interaction between DNA and Pt(II)(dppf)-complex

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Abstract

The interaction of Pt(II)(dppf)-complex, namely [Pt(dppf)(H2O)2]2+ with DNA was investigated by DPV and 1H-NMR techniques. The results showed that the interaction process has been characterized by changes in the electrochemical parameters of both compounds and the formation of a new anodic current peak close to the anodic current peak of the [Pt(dppf)(H2O)2]2+. In addition, the 1H-NMR spectra show that the coordination of Pt(II)(dppf)-complex to dsDNA occurs via N(7) of guanine. Others parameters like pH and ionic strength that affect the interaction process were also investigated.

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Acknowledgments

The authors thank FAPESP for the financial support.

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Authors and Affiliations

  1. Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Pampa, Campus Bagé, Bage, RS, Brazil

    Lucilene D. Mello

  2. Departamento de Química Inorgânica, UFRJ, Instituto de Química, Rio de Janeiro, RJ, Brazil

    Emerson S. Ribeiro

  3. UNICAMP, Instituto de Química, P.O. Box 6154, 13083-970, Campinas, SP, Brazil

    Lauro T. Kubota

  4. Biochemistry, Chemical Center, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Sofi K. C. Elmroth

  5. Pós-graduação em Farmácia, UNIBAN, Rua Maria Cândida, 1813, Vila Guilherme, São Paulo, SP, Brazil

    Regina M. S. Pereira

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  1. Lucilene D. Mello
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  2. Emerson S. Ribeiro
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  3. Lauro T. Kubota
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  4. Sofi K. C. Elmroth
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Correspondence to Regina M. S. Pereira.

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Mello, L.D., Ribeiro, E.S., Kubota, L.T. et al. Electrochemical and spectroscopic evidences of the interaction between DNA and Pt(II)(dppf)-complex. Biometals 22, 385–392 (2009). https://doi.org/10.1007/s10534-008-9175-2

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  • DOI: https://doi.org/10.1007/s10534-008-9175-2

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