Abstract
Experiments were conducted to investigate the effect of coal ash on organic acid exudation and subsequent metal uptake by ectomycorrhizal fungi. Four isolates of ectomycorrhizal fungi namely, Pisolithus tinctorius (EM-1293 and EM-1299), Scleroderma verucosum (EM-1283) and Scleroderma cepa (EM-1233) were grown on pond ash moistened with Modified Melin-Norkans medium in vitro. Exudation of formic acid, malic acid and succinic acid by these fungi were detected by HPLC. Mycelial accumulation of Al, As, Cd, Cr, Ni and Pb by these fungi was assayed by atomic absorption spectrophotometer. Relationship between organic acid exudation and metal uptake was determined using classical multivariate linear regression model. Correlation between organic acid exudation and metal uptake could be substantiated when several metals are considered collectively. The finding supports the widespread role of low molecular weight organic acid as a function of tolerance, when exposed to metals in vitro.
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Acknowledgments
The present study was supported by funding from Indo French Centre for the Promotion of Advanced Research (IFCPAR), project No 2109-1. The authors wish to thank Dr. R.K Pachauri, Director General, The Energy and Resources Institute, India for providing the infrastructure facilities. The authors also wish to thank Dr. Puneet Chitkara, Head, Department of Policy Studies, Teri University, for his help in doing statistical analysis.
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Ray, P., Adholeya, A. Correlation between organic acid exudation and metal uptake by ectomycorrhizal fungi grown on pond ash in vitro. Biometals 22, 275–281 (2009). https://doi.org/10.1007/s10534-008-9163-6
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DOI: https://doi.org/10.1007/s10534-008-9163-6