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Responsiveness to kainate in young rats after 2-week zinc deprivation

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Abstract

On the basis of the evidence of the enhanced susceptibility to kainate-induced seizures in young rats fed a zinc-deficient diet for 4 weeks, the relationship between zinc release from hippocampal neuron terminals and seizure susceptibility was studied in young rats fed the zinc-deficient diet for 2 weeks. Timm’s stain, with which histochemically reactive zinc in the presynaptic vesicle is detected, was not attenuated in mossy fibers and other areas in the hippocampus after 2-week zinc deprivation, whereas the attenuation was observed after 4-week zinc deprivation. Extracellular zinc concentration was not also decreased after 2-week zinc deprivation, unlike the case after 4-week zinc deprivation. To check the capacity for zinc release from neuron terminals after 2-week zinc deprivation, the hippocampus was excessively stimulated with 100 mM KCl. The increase in extracellular zinc concentration of zinc-deficient group was significantly more than that of control group. These results suggest that zinc release from hippocampal neuron terminals is not affected by 2-week zinc deprivation. On the other hand, the latency in myoclonic jerks of zinc-deficient group was significantly shorter than in the control group after treatment with kainate, while the latency in clonic convulsions was not different between the two groups. Intracellular fura-2 signal, a calcium indicator, was significantly higher in the hippocampal CA3 areas of zinc-deficient group 4 s after delivery of kainate to dentate granule cells. These results suggest that susceptibility to kainate-induced seizures is altered prior to the decrease in extracellular zinc concentration and zinc release from neuron terminals in zinc-deficient young rats. The alteration of calcium signaling seems to be involved in the susceptibility in zinc deficiency.

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Authors and Affiliations

  1. Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52- 1 Yada, Shizuoka, 422-8526, Japan

    Atsushi Takeda, Hiromasa Itoh, Haruna Tamano & Naoto Oku

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  1. Atsushi Takeda
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  2. Hiromasa Itoh
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  3. Haruna Tamano
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  4. Naoto Oku
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Correspondence to Atsushi Takeda.

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Takeda, A., Itoh, H., Tamano, H. et al. Responsiveness to kainate in young rats after 2-week zinc deprivation. Biometals 19, 565–572 (2006). https://doi.org/10.1007/s10534-005-6145-9

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  • DOI: https://doi.org/10.1007/s10534-005-6145-9

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