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Probing the adaptive response of Escherichia coli to extracellular Zn(II)

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Abstract

The adaptive response of Escherichia coli cells to differing intracellular and extracellular Zn(II) concentrations was evaluated by two-dimensional gel electrophoresis and peptide identifications. Twenty-one Zn(II)-responsive proteins, which were previously not known to be associated with Zn(II), were identified. Most of the proteins were related to cellular metabolism and include membrane transporters and glycolytic and TCA-associated enzymes. The expression levels of no known Zn(II) transporters were identified with these studies. The results of these studies suggest a role of Zn(II) in the expression levels of several E. coli proteins, and the results are discussed in light of recent genomic profiling studies on the adaptive response of E. coli cells to stress by Zn(II) excess.

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Abbreviations

ABC:

ATP-binding cassette

DEAE:

diethylaminoethyl

ICP-AES:

inductively coupled plasma atomic emission spectrometry

IEF:

isoelectric focusing

IPG:

immobilized pH gradient

MALDI-TOF MS:

matrix-assisted laser desorption ionization time-of-flight mass spectrometry

PMSF:

phenylmethylsulfonylfluoride

TEM:

transmission electron microscopy

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  1. Department of Chemistry and Biochemistry, Miami University, 112 Hughes Hall, Oxford, OH, 45056, USA

    Tara K. Sigdel, Renee Cilliers, Priya R. Gursahaney, Peter Thompson, J. Allen Easton & Michael W. Crowder

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  1. Tara K. Sigdel
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  2. Renee Cilliers
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  3. Priya R. Gursahaney
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  4. Peter Thompson
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  5. J. Allen Easton
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  6. Michael W. Crowder
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Correspondence to Michael W. Crowder.

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Sigdel, T.K., Cilliers, R., Gursahaney, P.R. et al. Probing the adaptive response of Escherichia coli to extracellular Zn(II). Biometals 19, 461–471 (2006). https://doi.org/10.1007/s10534-005-4962-5

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