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Proteomic detection of changes in protein synthesis induced by lanthanum in BGC-823 human gastric cancer cells

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Abstract

There is increasing interest in the use of rare earth elements in medicine. However, the biological mechanism of action of this metal ion remains unclear. In the present study, changes in protein synthesis induced by lanthanum in BGC-823 human gastric cancer cells were investigated. The proteins were separated using two-dimensional polyacrylamide gel electrophoresis and four proteins were significantly affected by lanthanum treatment when compared to an untreated control. Among them, one was down-regulated and three were up-regulated. Of these, three were successfully identified as RHOJ, CSP6 and MPI2 with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF-MS) after in-gel trypsin digestion. Among them, RHOJ was down-regulated and CSP6 and MPI2 were up-regulated. The three proteins are involved in various cellular functions, which are correlated with the regulation of cell morphology, gene transcription and cell cycle, respectively. It is suggested that the possible involvement of rare earth elements in the growth arrest of tumor cells is significantly associated with the differential protein expression induced by rare earth ions.

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References

  • LJ Angilheri (1979) ArticleTitleEffects of gallium and lanthanum on experimental tumor growth Eur J Cancer 15 1459–1462

    Google Scholar 

  • AC Bergman T Benjamin A Alaiya et al. (2000) ArticleTitleIdentification of gel-separated tumor marker proteins by mass spectrometry Electrophoresis 21 679–686 Occurrence Handle10.1002/(SICI)1522-2683(20000201)21:3<679::AID-ELPS679>3.0.CO;2-A Occurrence Handle1:CAS:528:DC%2BD3cXhslOqsr4%3D Occurrence Handle10726777

    Article  CAS  PubMed  Google Scholar 

  • YC Dai LI Jian LI Jie et al. (2002) ArticleTitleEffects of rare earth compounds on growth and apoptosis of leukemic cell lines In vitro Cell Dev Biol–An 38 373–375 Occurrence Handle1:CAS:528:DC%2BD3sXhtlKhsLw%3D

    CAS  Google Scholar 

  • M Deveci M Eski M Sengezer U Kisa (2000) ArticleTitleEffects of cerium nitrate bathing and promote burn wound excision on IL-6 and TNF-α levels in burned rats Burns 26 41–45 Occurrence Handle1:STN:280:DC%2BD3c%2FpsVSitg%3D%3D Occurrence Handle10630318

    CAS  PubMed  Google Scholar 

  • KI Galaktionov D Beach (1991) ArticleTitleSpecific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins Cell 67 1181–1194 Occurrence Handle10.1016/0092-8674(91)90294-9 Occurrence Handle1:CAS:528:DyaK3sXhs1Cjsbs%3D Occurrence Handle1836978

    Article  CAS  PubMed  Google Scholar 

  • K Galaktionov AK Lee J Eckstein G Draetta J Meckler M Loda D Beach (1996) ArticleTitleCdc25 phosphatases as potential human oncogenes Science 269 1575–1577

    Google Scholar 

  • WJ Henzed TM Stults SC Wong C Grimley C Watanabe (1993) ArticleTitleIdentifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases Proc Natl Acad Sci USA 90 5011–5015

    Google Scholar 

  • F Hong PL Wang K Wang RC Li (2003) ArticleTitleEffects of rare earths on proliferation toxicity of K562 and BEL-7402 cells J Chin Rare Earths Soc 21 597–599 Occurrence Handle1:CAS:528:DC%2BD2cXislShtQ%3D%3D

    CAS  Google Scholar 

  • M Ito C-X Yuan S Malik et al. (1999) ArticleTitleIdentity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators Mol Cell 3 361–370 Occurrence Handle10.1016/S1097-2765(00)80463-3 Occurrence Handle1:CAS:528:DyaK1MXit1OmsLo%3D Occurrence Handle10198638

    Article  CAS  PubMed  Google Scholar 

  • YJ Ji B Xiao ZH Wang MZ Cui YY Lu (2000) ArticleTitleThe suppression effect of light rare earth elements on proliferation of two cancer cell lines Biomed Environ Sci 13 287–292 Occurrence Handle1:STN:280:DC%2BD3M3ltlyltw%3D%3D Occurrence Handle11351862

    CAS  PubMed  Google Scholar 

  • AR Ketring (1987) ArticleTitle 153 Sm-EDTMP and 186 Re-HEDP as bone therapeutic radiopharmaceuticals Nucl Med Biol 14 223–232 Occurrence Handle1:CAS:528:DyaL2sXltFWrurk%3D

    CAS  Google Scholar 

  • UK Laemmli (1970) ArticleTitleCleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680–685 Occurrence Handle10.1038/227680a0 Occurrence Handle1:CAS:528:DC%2BD3MXlsFags7s%3D Occurrence Handle5432063

    Article  CAS  PubMed  Google Scholar 

  • C Lammer S Wagerer R Saffrcih D Mertens W Ansorge I Hoffmann (1998) ArticleTitleThe Cdc25B phosphatase is essential for the G2/M phase transition in human cell J Cell Sci 111 2445–2453 Occurrence Handle1:CAS:528:DyaK1cXmtVWqtbY%3D Occurrence Handle9683638

    CAS  PubMed  Google Scholar 

  • XB Li AR Zhou WH Yu XA Chen (1999) ArticleTitleThe effect of cerium compounds on two cell lines: human lung cancer cells PG and human gastric carcinoma cells BGC-823 Chin J Biochem Mol Biol 15 651–654 Occurrence Handle1:CAS:528:DyaK1MXlslGru74%3D

    CAS  Google Scholar 

  • XB Li AR Zhou WH Yu XA Chen (2000) ArticleTitleEffect of lanthanum citrate on two cell lines: human lung cancer cells PG and human carcinoma cells BGC-823 J Chin Rare Earths Soc 18 156–159

    Google Scholar 

  • T Sato M Hashizume Y Hotta Y Okahata (1998) ArticleTitleMorphology and proliferation of B16 melanoma cells in the presence of lanthanoid and Al 3+ ions Biometals 11 107–112 Occurrence Handle10.1023/A:1009273827598 Occurrence Handle1:CAS:528:DyaK1cXit1Wrtrk%3D Occurrence Handle9542064

    Article  CAS  PubMed  Google Scholar 

  • D Scheidegger BG Sparkes N Lüscher GA Schoenenberger M Allgöwer (1992) ArticleTitleSurvival in major burn injuries treated by one bathing in cerium nitrate Burns 18 296–300 Occurrence Handle10.1016/0305-4179(92)90150-S Occurrence Handle1:STN:280:ByyD3sbltlw%3D Occurrence Handle1418505

    Article  CAS  PubMed  Google Scholar 

  • E Vignal M De Toledo F Comunale et al. (2000) ArticleTitleCharacterization of TCL, a new GTPase of the Rho family related to TC10 and Cdc42 J Biol Chem 275 36457–36464 Occurrence Handle10.1074/jbc.M003487200 Occurrence Handle1:CAS:528:DC%2BD3cXosVSrsbs%3D Occurrence Handle10967094

    Article  CAS  PubMed  Google Scholar 

  • CL Wu JS Liu WD Yang ZG Sheng (2003) ArticleTitleEffects of La(NO3)3 on human neuroglioma cells J Chin Rare Earths Soc 21 321–325

    Google Scholar 

  • B Xiao YJ Ji ZH Wang et al. (1997) ArticleTitleEffect of lanthanum and cerium on malignant proliferation and expression of tumor-related gene Chin J Prev Med 31 228–230 Occurrence Handle1:CAS:528:DyaK1cXjtV2hu7s%3D

    CAS  Google Scholar 

Download references

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Correspondence to Ping Shi.

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Published online December 2004

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Shi, P., Huang, Z. Proteomic detection of changes in protein synthesis induced by lanthanum in BGC-823 human gastric cancer cells. Biometals 18, 89–95 (2005). https://doi.org/10.1007/s10534-004-1812-9

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  • DOI: https://doi.org/10.1007/s10534-004-1812-9

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