Abstract
Quinolobactin is a new siderophore produced by a pyoverdine deficient mutant of Pseudomonas fluorescens. A simple and efficient synthesis of quinolobactin is described, starting from xanthurenic acid. The protonation constants of quinolobactin were determined by potentiometric titrations as pKa2=5.50 ± 0.07, pKa1=10.30 ± 0.05. The equilibria of the metal complexes were studied by means of spectrophotometric and potentiometric titrations. The overall stability constants of the quinolobactin-FeIII complexes was found to be log β111=18.60 ± 0.10, log β121=32.60 ± 0.20, log β120=28.20 ± 0.25 resulting in a pFeIII value of 18.2 at pH 7.4. The UV-visible spectral parameters of the [FeL2] are in agreement with a complex containing two ligands coordinated to one Fe3+ cation through the oxygen and nitrogen quinoline atoms.
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du Moulinet d'Hardemare, A., Serratrice, G. & Pierre, JL. Synthesis and iron-binding properties of quinolobactin, a siderophore from a pyoverdine-deficient Pseudomonas fluorescens . Biometals 17, 691–697 (2004). https://doi.org/10.1007/s10534-004-1205-0
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DOI: https://doi.org/10.1007/s10534-004-1205-0