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Dominant tree mycorrhizal associations affect soil nitrogen transformation rates by mediating microbial abundances in a temperate forest

Biogeochemistry volume 158, pages 405–421 (2022)Cite this article

Abstract

Tree–fungal symbioses are increasingly recognized to affect soil nitrogen (N) transformations, yet the role of free-living soil microbes in the process is largely unclear. Soil microbes directly interact with trees and are a primary driver of many N transformation processes. Here, we explored the linkage among tree mycorrhizal associations, free-living soil microbes and N transformation rates in a temperate forest of Northeast China. Across a gradient of increasing ectomycorrhizal (ECM) tree dominance, we measured soil acid–base chemistry, bacterial and fungal abundances, N-hydrolyzing enzyme activities, abundances and community composition of ammonia-oxidizing archaea (AOA) and bacteria, and net N mineralization and net nitrification rates. Results showed that soil pH, exchangeable base cations, inorganic N concentrations and N transformation rates decreased with increasing ECM tree dominance. The ECM tree dominance was negatively related to soil bacterial and AOA amoA gene abundances, and positively to soil fungal abundances and β-N-acetylglucosaminidase activities. These shifts in soil microbial abundances and enzyme activities along the mycorrhizal gradient were linked with the increase in soil acidity with increasing ECM tree dominance. Piecewise structural equation models revealed that ECM tree dominance was not directly related to N transformation rates, but indirectly to net N mineralization rates by affecting bacterial and fungal abundances, and indirectly to net nitrification rates by influencing AOA amoA gene abundances. Collectively, our results indicate that soil microbes provide a mechanistic link between mycorrhizal associations and soil N transformations, and suggest that shifts in forest mycorrhizal associations under global change could have profound consequences for biogeochemical cycling of temperate forests.

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Data availability

The datasets generated during and/or analyzed during the current study are available from corresponding authors on reasonable request.

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Acknowledgements

We are grateful to the National Research Station of Changbai Mountain Forest Ecosystems for providing the experimental site and relevant supports. We thank Dr. Stephen Sebestyen and two anonymous reviewers for suggestions that greatly improve our manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (Nos. 31700538 and 31830015) and the Youth Innovation Promotion Association CAS (No. 2019200).

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Authors and Affiliations

  1. CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China

    Guigang Lin, Zuoqiang Yuan, Yansong Zhang, De-Hui Zeng & Xugao Wang

  2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China

    Yansong Zhang

  3. Liaoning Provincial Key Laboratory of Terrestrial Ecosystem Carbon Neutrality, Shenyang, 110016, China

    Xugao Wang

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  1. Guigang Lin
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  2. Zuoqiang Yuan
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  3. Yansong Zhang
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  4. De-Hui Zeng
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GL, DZ, XW conceived the study; GL, ZY and YZ collected and analyzed the data; All authors contributed critically to the drafts and gave final approval for publication.

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Correspondence to Xugao Wang.

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Lin, G., Yuan, Z., Zhang, Y. et al. Dominant tree mycorrhizal associations affect soil nitrogen transformation rates by mediating microbial abundances in a temperate forest. Biogeochemistry 158, 405–421 (2022). https://doi.org/10.1007/s10533-022-00909-9

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Keywords

  • Ammonia oxidizer
  • Fungal:bacterial ratio
  • Hydrolytic enzyme
  • Mycorrhizal type
  • Plant–soil interaction
  • Soil acid–base chemistry