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Methane oxidation kinetics in northern freshwater lakes

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Abstract

Understanding the drivers of aerobic methane (CH4) oxidation (MOX) is paramount in assessing the current and potential future CH4 emissions from freshwater aquatic systems. Regulation of MOX kinetics is a complex function of CH4 and oxygen (O2) concentrations. While MOX activity is usually proportional to the concentration of CH4 itself, the effects of O2 have been more conflicting, with maximum MOX rates often restricted to low O2 concentrations. Despite the complexity involved, MOX kinetics are often modelled as monotonic positive functions of both CH4 and O2 concentrations. We conducted a series of incubation experiments using natural and unamended water samples obtained from multiple depths in northern temperate lakes that vary widely and independently in their CH4 and O2 concentrations. Our results showed the expected positive effect of CH4 concentration and temperature but also demonstrated the strong inhibitory effects of O2 at high concentration. We then developed a general model describing the kinetics of MOX, simultaneously integrating the effects of CH4 concentration, temperature as well as the non-linear effect O2 on MOX activity. The model revealed an overall temperature dependency (activation energy = 0.49 ± 0.06 eV) much lower than reported for methanogenesis and an optimal O2 level around 15 μmol O2 L−1 where maximum MOX activity occurs, regardless of CH4 concentration and temperature. We further show that ignoring the inhibitory effect of O2 can lead to significant bias in calculating the expected MOX rates in different portions of the water column.

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Acknowledgements

We thank Brenden Chabot and Katherine Velghe for assistance in the field and laboratory. We also thank the staff at the Station de biologie des Laurentides (Université de Montréal) for assistance. This study was supported by Natural Sciences and Engineering Research Council of Canada Discovery grants to YTP and is a contribution to UNESCO Chair in Global Environmental Change. SDT and PCJR are supported by doctoral scholarships from Fonds de recherche Nature et Technologies du Québec.

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  1. Groupe de Recherche Interuniversitaire en Limnologie, Département des Sciences Biologiques, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, Montréal, QC, H3C 3P8, Canada

    Shoji D. Thottathil, Paula C. J. Reis & Yves T. Prairie

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Thottathil, S.D., Reis, P.C.J. & Prairie, Y.T. Methane oxidation kinetics in northern freshwater lakes. Biogeochemistry 143, 105–116 (2019). https://doi.org/10.1007/s10533-019-00552-x

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