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Biogeochemistry

, Volume 133, Issue 3, pp 347–364 | Cite as

Using oxygen stable isotopes to quantify ecosystem metabolism in northern lakes

  • Matthew J. Bogard
  • Dominic Vachon
  • Nicolas F. St.-Gelais
  • Paul A. del Giorgio
Article

Abstract

In remote regions of the world, whole lake metabolic estimates are scarce, largely because long incubations, intensive sampling and deployment of monitoring equipment are impractical. The oxygen isotope (δ18O) mass balance approach represents a simple and efficient alternative to measure whole-lake gross primary production (GPP) and respiration (R) from a single point sample, yet this option has not been extensively explored in habitats such as remote northern lakes. Here, we explored the applicability of the method using a sensitivity analysis on simulated data, showing that in large, heterotrophic (i.e., R > GPP) lakes, model outputs are sensitive to input terms for isotopic fractionation and air–water gas exchange. Despite these sensitivities, field applications of the δ18O method generated promising results that were generally consistent with parallel, free-water diel DO metabolic modelling, but greater than in vitro incubation measurements. The isotopic approach captured both wide-ranging metabolic conditions in in situ experimental mesocosms, and the seasonal trends in GPP and R in a shallow, dystrophic lake. In a clearer, deeper heterotrophic lake, the isotope approach integrated a fraction of metalimnetic metabolism missed by diel DO metabolic estimates. Overall, metalimnetic contributions to surface δ18O–DO dynamics had the greatest impact on model outputs, but with accurate information on air–water gas exchange, mixing depth, and the vertical DO and light regime of a given system, these effects can be accounted for and the isotopic approach can yield well constrained, spatio-temporally integrated rates of GPP and R. The approach is clearly suitable for use in oligo- and mesotrophic lakes, especially in remote regions where sampling is logistically difficult.

Keywords

Metabolism Oxygen Stable isotope Primary production Respiration Lake Net ecosystem production 

Notes

Acknowledgements

We thank Carolina Garcia Chaves, Simon Gauthier-Fautaux, Juan Pablo Nino Garcia, Cynthia Soued, Marilyne Robidoux, and Anthony Merante for field and laboratory assistance, and Alison Derry for use of the mesocosms. Annick St. Pierre, Alice Parks and the employees of the Station de biologie des Laurentides de l’Université de Montréal provided logistical support. We thank Amber Ulseth, Erin Hotchkiss, and Yves Prairie for helpful discussions on lake metabolism and the use of DO stable isotopes, Biel Obrador for providing published data, and Bob Hall plus one anonymous reviewer for providing constructive comments that improved the paper. M.J.B. was supported by doctoral grants from the National Science and Engineering Research Council of Canada (NSERC) and the Université du Québec a Montréal. This project is part of the program of the NSERC/HQ Industrial Research Chair in Carbon Biogeochemistry in Boreal Aquatic Systems (CarBBAS), co-funded by grants from NSERC and Hydro-Québec (to P.A.d.G.).

Supplementary material

10533_2017_338_MOESM1_ESM.docx (214 kb)
Supplementary material 1 (DOCX 213 kb)

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Matthew J. Bogard
    • 1
    • 2
  • Dominic Vachon
    • 1
    • 3
  • Nicolas F. St.-Gelais
    • 1
  • Paul A. del Giorgio
    • 1
  1. 1.Groupe de Recherche Interuniversitaire En Limnologie, Département des Sciences BiologiquesUniversité du Québec à MontréalMontréalCanada
  2. 2.School of Environmental and Forest Sciences, College of the EnvironmentUniversity of WashingtonSeattleUSA
  3. 3.Department F.-A. Forel for Environmental and Aquatic Sciences (DEFSE), Faculty of ScienceUniversity of GenevaGenevaSwitzerland

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