Abstract
Rates and pathways of methane production were determined from photosynthetic soft microbial mats and gypsum-encrusted endoevaporites collected in hypersaline environments from California, Mexico and Chile, as well as an organic-rich mud from a pond in the El Tatio volcanic fields, Chile. Samples (mud, soft mats and endoevaporites) were incubated anaerobically with deoxygenated site water, and the increase in methane concentration through time in the headspaces of the incubation vials was used to determine methane production rates. To ascertain the substrates used by the methanogens, 13C-labeled methylamines, methanol, dimethylsulfide, acetate or bicarbonate were added to the incubations (one substrate per vial) and the stable isotopic composition of the resulting methane was measured. The vials amended with 13C-labeled methylamines produced the most 13C-enriched methane, generally followed by the 13C-labeled methanol-amended vials. The stable isotope data and the methane production rates were used to determine first order rate constants for each of the substrates at each of the sites. Estimates of individual substrate use revealed that the methylamines produced 55–92 % of the methane generated, while methanol was responsible for another 8–40 %.
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Acknowledgments
Funding by the NASA Exobiology program is gratefully acknowledged. We would also like to thank Angela Detweiler, Adrienne Frisbee, Amanda Tazaz, Tyler Mauney, Jennifer Poole, Brooke Nicholson, Claire Beaudoin, and Alfonso Davila, as well as our many colleagues in Mexico and Chile, for help in the field and laboratory. We are also appreciative of the access to the field sites provided by Exportadora de Sal, S.A. de C.V and the U.S. Fish and Wildlife Service. Neal Blair is thanked for his thoughtful comments on an earlier version of the manuscript.
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Kelley, C.A., Chanton, J.P. & Bebout, B.M. Rates and pathways of methanogenesis in hypersaline environments as determined by 13C-labeling. Biogeochemistry 126, 329–341 (2015). https://doi.org/10.1007/s10533-015-0161-9
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DOI: https://doi.org/10.1007/s10533-015-0161-9