Biogeochemistry

, Volume 120, Issue 1–3, pp 295–305 | Cite as

Microbial controls on DMSP degradation and DMS formation in the Sargasso Sea

  • Maria Vila-Costa
  • Johanna M. Rinta-Kanto
  • Rachel S. Poretsky
  • Shulei Sun
  • Ronald P. Kiene
  • Mary Ann Moran
Article

Abstract

Bacterial degradation of dimethylsulfoniopropionate (DMSP) represents one of the main sources of the climatically–active trace gas dimethylsulfide (DMS) in the upper ocean. Short-term enrichment studies to stimulate specific pathways of DMSP degradation in oligotrophic waters from the Sargasso Sea were used to explore regulatory connections between the different bacterial DMSP degradation steps and determine potential biological controls on DMS formation in the open ocean. Experiments were conducted with surface water at the BATS station in the western North Atlantic Ocean. We added selected organic substrates (25 nmol L−1 final concentration) to induce different steps of DMSP degradation in the microbial community, and then measured DMSP dynamics (assimilation and turnover rates), DMS yields (using 35sulfur-DMSP tracer), and bacterial production rates. In most treatments, the main fate of consumed S-DMSP was excretion as a non-volatile S product. 35S-DMSP tracer turnover rates (accumulation + assimilation + excretion of transformed products as DMS or others) increased upon addition of DMSP and glucose, but not acrylate, methymercaptopropionate (MMPA), methanethiol, DMS or glycine betaine. DMS yields from 35S-DMSP never exceeded 16 % except in a short term DMSP enrichment, for which the yield reached 45 % (±17 %). Results show that availability of non-sulfur containing labile C sources (glucose, acrylate) decreased bacterial DMS production while stimulating bacterial heterotrophic production, and suggest an influence of bacterial sulfur demand in controlling DMS-yielding pathways. However, regulatory effects on 35S-DMSP fate were not consistent across all reduced sulfur compounds (i.e., methanethiol or MMPA), and may reflect alternate roles of DMSP as a bacterial energy source and osmolyte.

Keywords

Dimethylsulfoniopropionate DMSP Dimethylsulfide DMS Sargasso sea Bacterioplankton 

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Maria Vila-Costa
    • 1
    • 2
    • 3
  • Johanna M. Rinta-Kanto
    • 1
    • 5
  • Rachel S. Poretsky
    • 1
    • 6
  • Shulei Sun
    • 1
    • 7
  • Ronald P. Kiene
    • 4
  • Mary Ann Moran
    • 1
  1. 1.Department of Marine SciencesUniversity of GeorgiaAthensUSA
  2. 2.Limnological Observatory of the Pyrenees (LOOP) – Department of EcologyUniversity of BarcelonaBarcelonaSpain
  3. 3.Department of Environmental ChemistryIDAEA-CSICBarcelonaSpain
  4. 4.Department of Marine SciencesUniversity of South AlabamaMobileUSA
  5. 5.Division of Microbiology, Department of Food and Environmental SciencesUniversity of HelsinkiHelsinkiFinland
  6. 6.Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA
  7. 7.Center for Research in Biological SystemsUniversity of California San DiegoLa JollaUSA

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