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Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight

Abstract

Polyamines are short-chain aliphatic compounds containing multiple amine groups. They are important components of the cytosol of eukaryotes and are present at mmol L−1 concentrations inside phytoplankton cells, while complex polyamines play a role in biosilica deposition. Concentrations of polyamines measured in seawater are typically in the sub-nmol L−1 range, implying rapid and efficient uptake by osmotrophs, likely bacterioplankton. We measured turnover rates of three polyamines (putrescine, spermidine and spermine) using 3H-labeled compounds and determined their concentrations by HPLC to estimate polyamine contributions to dissolved organic matter and bacterioplankton carbon and nitrogen demand. These measurements were made on transects from the inner shelf to the Gulf Stream across the South Atlantic Bight (SAB) during April and October of 2011 and in salt marsh estuaries on the Georgia coast during August of 2011 and April of 2012. We found that turnover rates of polyamines were similar to those of amino acids (arginine and glutamic acid) measured in the same samples; however, fluxes of polyamines into bacterioplankton were much lower than amino acid fluxes as a result of low ambient concentrations. Turnover rates and fluxes of polyamines decreased from near-shore waters to the shelf-break, following the pattern of chlorophyll a concentration. Polyamine uptake accounted for less than 10 % of bacterial N demand and 5 % of bacterial C demand on average, with a large variation among water masses.

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Acknowledgments

We thank the captain and crew of the R/V Savannah for their assistance with sample collection during two cruises, as well as Anna M. Bratcher for assisting with processing the nutrient data presented here. We would also like to thank the crew of the R/V Salty Dawg, especially Jacob Shalack, for assistance with sample collections at GCE-LTER sites, as well as Mary Price and Gracie Townsend for helping with facilities and equipment at UGAMI. This research was funded by the National Science Foundation (NSF OCE 1029742 to J.T.H and OCE 1029607 to X.M.).

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Correspondence to James T. Hollibaugh.

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Responsible Editor: Dr. Mark Brush.

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10533_2014_56_MOESM2_ESM.pdf

Fig. S1 (a) Stations sampled during April (18–22) and October (2–6) cruises in the South Atlantic Bight (SAB). Stations visited on both cruises are represented with diamonds (purple); stations visited only on the April cruise represented with squares (blue) and those visited only on the October cruise represented with circles (red). (b) GCE-LTER sites located on the central Georgia coast and bounded on the east by the SAB. Samples were collected from the Sapelo Sound (SP, GCE 1, 2 and 3), Marsh Landing (ML) and Altamaha Sound (AL, GCE 7, 8 and 9) during 13–16 August 2011 and 17–20 April 2012. Fig. S2 Relative fluorescence (mg m−3), concentrations of biogenic silica (BSi, µmol L−1), l-leucine incorporation rates (LEU, pmol h−1 L−1) and bacterial 16S rRNA gene abundance (copies L−1) in samples collected on the April (a, b) and October (c, d) cruises. Numbers on the abscissa identify the station as per Figure S1a. SW, surface water; MW, mid-water; BW, bottom water (PDF 1001 kb)

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Liu, Q., Lu, X., Tolar, B.B. et al. Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight. Biogeochemistry 123, 117–133 (2015). https://doi.org/10.1007/s10533-014-0056-1

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  • DOI: https://doi.org/10.1007/s10533-014-0056-1

Keywords

  • Polyamines
  • Dissolved organic nitrogen
  • Bacterioplankton
  • Spermidine
  • Spermine
  • Putrescine
  • DFAA
  • South Atlantic Bight