, Volume 122, Issue 2–3, pp 313–326 | Cite as

Effects of calcium silicate treatment on the composition of forest floor organic matter in a northern hardwood forest stand

  • Ankit Balaria
  • Chris E. JohnsonEmail author
  • Peter M. Groffman
  • Melany C. Fisk


Calcium amendment can help improve forest sustainability in stands that have been impacted by chronic acid deposition. An important component of this improvement is the stimulation of the microbial activity that supports ecosystem nutrient cycling processes. To test the hypothesis that Ca treatment alters the structure and solubility of organic matter substrates, an important driver of microbial activity, we investigated the effect of wollastonite (CaSiO3) treatment on soil organic matter (SOM) and hot-water-extractable organic matter (HWEOM). We found a decrease in the HWEOM content of forest floor soils within 2 years of treatment with a high dosage of wollastonite (4,250 kg Ca/ha), but not at a low dosage (850 kg Ca/ha). High-dosage treatment did not reduce the biodegradability of HWEOM. Hence, a high dose of CaSiO3 appears to reduce the solubility of organic matter in the forest floor but not the bioavailability of the extracted SOM. Nuclear magnetic resonance spectroscopy revealed no significant changes in the O-alkyl C content of SOM in response to wollastonite addition, but a reduction in the O-alkyl C content of HWEOM suggests that the extractability of carbohydrate structures was reduced by added CaSiO3. Phosphorous treatment, when performed in combination with Ca, also decreased the O-alkyl C content of HWEOM, but had no effect when performed without Ca. The reduced solubility of SOM after Ca treatment may have been the result of bridging between Ca2+ and negatively charged sites on SOM, as suggested in other studies. Also, high concentrations of Si in soil solution, due to dissolution of the wollastonite, likely resulted in oversaturated conditions with respect to SiO2 or kaolinite, perhaps leading to co-precipitation of soluble organic matter. Overall, our results suggest that added Ca and/or Si may react with SOM to reduce the accessibility of labile C forms to soil microbes.


Calcium Forest soil Hot-water extractable organic matter Nuclear magnetic resonance spectroscopy Phosphorus Soil carbon Soil organic matter 



We gratefully acknowledge the United States Department of Agriculture (USDA) NRI Competitive Grants Program (award no. 2005-35107-16200) and the National Science Foundation Long-Term Ecological Research Program (Grant No. 1114804) for support of this research. Ankit Balaria held the Wen-Hsiung and Kuan-Ming Li Graduate Fellowship in the Department of Civil and Environmental Engineering at Syracuse University while conducting this research. We appreciate the help of Mary Margaret Koppers, Mario Montesdeoca, Lisa Martel, Colin Fuss, and David Kiemle. This is a contribution to the Hubbard Brook Ecosystem Study. The Hubbard Brook Experimental Forest is administered by the USDA Forest Service Northern Research Station, Newtown Square, PA.


  1. Adams SN, Cooper JE, Dickson DA, Dickson EL, Seaby DA (1978) Some effects of lime and fertilizer on a Sitka spruce plantation. Forestry 51:57–65CrossRefGoogle Scholar
  2. Baath E, Berg B, Lohm U, Lundgren B, Lundkvist H, Rosswall T, Soderstrom B, Wiren A (1980) Effects of experimental acidification and liming on soil organisms and decomposition in a Scots pine forest. Pedobiologia 28:85–100Google Scholar
  3. Badalucco L, Grego S, Dell’Orco S, Nannipieri P (1992) Effect of liming on some chemical, biochemical, and microbiological properties of acid soils under spruce (Picea abies L.). Biol Fertil Soils 14:76–83CrossRefGoogle Scholar
  4. Balaria A, Johnson CE (2013) Compositional characterization of soil organic matter and hot-water-extractable organic matter in organic horizons using a molecular mixing model. J Soils Sediments 13:1032–1042CrossRefGoogle Scholar
  5. Balaria A, Johnson CE, Xu Z (2009) Molecular-scale characterization of hot-water extractable organic matter in a northeastern forest soil. Soil Sci Soc Am J 73:812–821CrossRefGoogle Scholar
  6. Battles JJ, Fahey TJ, Driscoll CT, Blum JD, Johnson CE (2014) Restoring soil calcium reverses forest decline. Environ Sci Technol  Lett 1:15–19CrossRefGoogle Scholar
  7. Belkacem S, Nys C (1995) Consequences of liming and gypsum top-dressing on nitrogen and carbon dynamics in acid forest soils with different humus forms. Plant Soil 173:79–88CrossRefGoogle Scholar
  8. Bohlen PJ, Groffman PG, Driscoll CT, Fahey TJ, Siccama TG (2001) Plant–soil–microbial interactions in a northern hardwood forest. Ecology 82:965–978Google Scholar
  9. Chen CR, Xu Z, Mathers NJ (2004) Soil carbon pools in adjacent natural and plantation forests of subtropical Australia. Soil Sci Soc Am J 68:282–291CrossRefGoogle Scholar
  10. Cho Y, Driscoll CT, Johnson CE, Siccama TG (2010) Chemical changes in soil and soil solution from calcium silicate addition to a northern hardwood forest. Biogeochemistry 100:3–20CrossRefGoogle Scholar
  11. Cho Y, Driscoll CT, Johnson CE, Blum JD, Fahey TJ (2012) Watershed-level responses to calcium silicate treatment in a northern hardwood forest. Ecosystems 15:416–434CrossRefGoogle Scholar
  12. Cleveland CC, Townsend AR, Schmidt SK (2002) Phosphorus limitation of microbial processes in moist tropical forests; evidence from short-term laboratory incubations and field studies. Ecosystems 5:680–691CrossRefGoogle Scholar
  13. Driscoll CT, Lawrence GB, Bulger TJ, Butler CS, Cornan CS, Eager C, Lambert KF, Likens GE, Stoddard JL, Weathers KC (2001) Acidic deposition in the northeastern United States: sources and inputs, ecosystem effects, and management strategies. Bioscience 51:180–198CrossRefGoogle Scholar
  14. Filep T, Szili-Kovács T (2010) Effect of liming on microbial biomass carbon of acidic arenosols in pot experiments. Plant Soil Environ 56:268–273Google Scholar
  15. Fiorentino I, Fahey TJ, Groffman PM, Driscoll CT, Eager C, Siccama TG (2003) Initial response of phosphorous biogeochemistry to calcium addition in a northern hardwood forest ecosystem. Can J For Res 33:1864–1873CrossRefGoogle Scholar
  16. Frostegard A, Baath E, Tunlid A (1993) Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analyses. Soil Biol Biochem 25:723–730CrossRefGoogle Scholar
  17. Ghani A, Dexter M, Perrott KW (2003) Hot-water extractable carbon in soils: a sensitive measurement for determining impacts of fertilization, grazing and cultivation. Soil Biol Biochem 35:1231–1243CrossRefGoogle Scholar
  18. Griffin EM (1985) A comparison of the roles of bacteria and fungi. In: Leadbetter ER, Poindexter JS (eds) Bacteria in Nature, vol. 1. Bacterial activities in perspective. Plenum Press, New York, pp 221–255Google Scholar
  19. Groffman PM, Fisk MC (2011a) Phosphate additions have no effect on microbial biomass and activity in a northern hardwood forest. Soil Biol Biochem 43:2441–2449CrossRefGoogle Scholar
  20. Groffman PM, Fisk MC (2011b) Calcium constrains plant control over forest ecosystem nitrogen cycling. Ecology 92:2035–2042CrossRefGoogle Scholar
  21. Groffman PM, Fisk CF, Driscoll CT, Likens GE, Fahey TJ, Eager C, Pardo LH (2006) Calcium additions and microbial nitrogen cycle processes in a northern hardwood forest. Ecosystems 9:1289–1305CrossRefGoogle Scholar
  22. Hawley GJ, Schaberg PG, Eager C, Borer CH (2006) Calcium addition at the Hubbard Brook Experimental Forest reduced winter injury to red spruce in a high-injury year. Can J For Res 36:1–6CrossRefGoogle Scholar
  23. Haynes RJ, Swift RS (1988) Effect of lime and phosphate additions on changes in enzyme activities, microbial biomass and levels of extractable nitrogen, sulphur and phosphorous in an acid soil. Biol Fert Soils 6:153–158CrossRefGoogle Scholar
  24. Hobbie SE, Vitousek PM (2000) Nutrient limitation of decomposition in Hawaiian forests. Ecology 81:1867–1877CrossRefGoogle Scholar
  25. Hobbie SE, Miley TA, Weiss MS (2002) Carbon and nitrogen cycling in soils from acidic and nonacidic tundra with different glacial histories in northern Alaska. Ecosystems 5:761–775CrossRefGoogle Scholar
  26. Illmer P, Schinner F (1991) Effects of lime and nutrient salts on the microbiological activities of forest soils. Biol Fert Soils 11:261–266CrossRefGoogle Scholar
  27. Ivarson KC (1977) Changes in decomposition rate, microbial population and carbohydrate content of an acid peat bog after liming and reclamation. Can J Soil Sci 57:129–137CrossRefGoogle Scholar
  28. Johnson CE, Johnson AH, Siccama TG (1991) Whole-tree clearcutting effects on exchangeable cations and soil acidity. Soil Sci Soc Am J 55:502–508CrossRefGoogle Scholar
  29. Johnson CE, Driscoll CT, Blum JD, Fahey TJ, Battles JJ (2014) Soil chemical dynamics after calcium silicate addition to a northern hardwood forest. Soil Sci Soc Am J 78:1458–1468CrossRefGoogle Scholar
  30. Juice SM, Fahey TJ, Siccama TG, Driscoll CT, Denny EG, Eager C, Cleavitt NL, Minocha R, Richardson AD (2006) Response of sugar maple to calcium addition to northeastern hardwood forest. Ecology 87:1267–1280CrossRefGoogle Scholar
  31. Kreutzer K (1995) Effects of forest liming on soil processes. Plant Soil 168–169:447–470CrossRefGoogle Scholar
  32. Likens GE, Driscoll CT, Buso DC, Siccama TG, Johnson CE, Lovett GM, Ryan DF, Fahey TJ, Reiners WA (1998) The biogeochemistry of calcium at Hubbard Brook. Biogeochemistry 41:89–173CrossRefGoogle Scholar
  33. Lohm U, Larsson K, Nömmik H (1984) Acidification and liming of coniferous forest soil: long-term effects on turnover rates of carbon and nitrogen during an incubation experiment. Soil Biol Biochem 16:343–346CrossRefGoogle Scholar
  34. Melvin AM, Lichstein JW, Goodale CL (2013) Forest liming increases forest floor carbon and nitrogen stocks in a mixed hardwood forest. Ecol Appl 23:1962–1975CrossRefGoogle Scholar
  35. Minick KJ, Fisk MC, Groffman PM (2011) Calcium and phosphorus interact to reduce mid-growing season net nitrogen mineralization potential in organic horizons in a northeastern hardwood forest. Soil Biol Biochem 43:271–279CrossRefGoogle Scholar
  36. Nannipieri P, Johnson RL, Paul EAB (1978) Criteria for measurement of microbial growth and activity in soil. Soil Biol Biochem 10:223–228CrossRefGoogle Scholar
  37. Neale SP, Shah Z, Adams WA (1997) Changes in microbial biomass and nitrogen turnover in acidic organic soils following liming. Soil Biol Biochem 29:1463–1474CrossRefGoogle Scholar
  38. Nelson PN, Baldock JA (2005) Estimating the molecular composition of a diverse range of natural organic materials from solid-state 13C NMR and elemental analyses. Biogeochemistry 72:1–34CrossRefGoogle Scholar
  39. Persson T, Lundkvist H, Wiren A, Hyvonen R, Wessen B (1989) Effects of acidification and liming on carbon and nitrogen mineralization and soil organisms in mor humus. Water Air Soil Pollut 45:77–96Google Scholar
  40. Peters SC, Blum JD, Driscoll CT, Likens GE (2004) Dissolution of wollastonite during the experimental manipulation of Hubbard Brook Watershed 1. Biogeochemistry 67:309–329CrossRefGoogle Scholar
  41. Poozesh V, Castillon P, Cruz P, Bertoni G (2010) Re-evaluation of the liming fertilization interaction in grassland on poor and acid soils. Grass Forage Sci 65:260–272CrossRefGoogle Scholar
  42. Speir TW, Ross DJ (1978) Soil phosphatase and sulphatase. In: Burns RG (ed) Soil Enzymes. Academic Press, London, pp 197–250Google Scholar
  43. Yavitt JB, Newton RM (1990) Liming effects on some chemical and biological parameters of soil (spodosols and histosols) in a hardwood forest watershed. Water Air Soil Pollut 54:529–544CrossRefGoogle Scholar
  44. Zelles L, Scheunert I, Kreutzer K (1978) Bioactivity in limed soil of a spruce forest. Biol Fert Soils 3:211–216CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Ankit Balaria
    • 1
  • Chris E. Johnson
    • 1
    Email author
  • Peter M. Groffman
    • 2
  • Melany C. Fisk
    • 3
  1. 1.Department of Civil and Environmental EngineeringSyracuse UniversitySyracuseUSA
  2. 2.Cary Institute of Ecosystem StudiesMillbrookUSA
  3. 3.Department of BiologyMiami UniversityOxfordUSA

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