, Volume 117, Issue 1, pp 5–21 | Cite as

Extracellular enzymes in terrestrial, freshwater, and marine environments: perspectives on system variability and common research needs

  • C. Arnosti
  • C. Bell
  • D. L. Moorhead
  • R. L. Sinsabaugh
  • A. D. Steen
  • M. Stromberger
  • M. Wallenstein
  • M. N. Weintraub


Extracellular enzymes produced by heterotrophic microbial communities are major drivers of carbon and nutrient cycling in terrestrial, freshwater, and marine environments. Although carbon and nutrient cycles are coupled on global scales, studies of extracellular enzymes associated with terrestrial, freshwater, and marine microbial communities are not often compared across ecosystems. In part, this disconnect arises because the environmental parameters that control enzyme activities in terrestrial and freshwater systems, such as temperature, pH, and moisture content, have little explanatory power for patterns of enzyme activities in marine systems. Instead, factors such as the functional diversity of microbial communities may explain varying patterns of enzyme activities observed in the ocean to date. In any case, many studies across systems focus on similar issues that highlight the commonalities of microbial community organization. Examples include the effective lifetime of enzymes released into the environment; the extent to which microbial communities coordinate enzyme expression to decompose complex organic substrates; and the influence of microbial community composition on enzyme activities and kinetics. Here we review the often-disparate research foci in terrestrial, freshwater, and marine environments. We consider the extent to which environmental factors may regulate extracellular enzyme activities within each ecosystem, and highlight commonalities and current methodological challenges to identify research questions that may aid in integrating cross-system perspectives in the future.


Extracellular enzymes Soil Aquatic Terrestrial Marine Microbial communities 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • C. Arnosti
    • 1
  • C. Bell
    • 2
  • D. L. Moorhead
    • 3
  • R. L. Sinsabaugh
    • 4
  • A. D. Steen
    • 5
  • M. Stromberger
    • 6
    • 7
  • M. Wallenstein
    • 2
  • M. N. Weintraub
    • 3
  1. 1.Department of Marine SciencesUniversity of North Carolina-Chapel HillChapel HillUSA
  2. 2.Natural Resource Ecology LaboratoryColorado State UniversityFort CollinsUSA
  3. 3.Department of Environmental SciencesUniversity of ToledoToledoUSA
  4. 4.University of New MexicoAlbuquerqueUSA
  5. 5.Department of MicrobiologyUniversity of TennesseeKnoxvilleUSA
  6. 6.Department of Soil and Crop SciencesColorado State UniversityFort CollinsUSA
  7. 7.Graduate Degree Program in EcologyColorado State UniversityFort CollinsUSA

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