Biogeochemistry

, Volume 96, Issue 1–3, pp 189–208 | Cite as

Nitrification and denitrification in a midwestern stream containing high nitrate: in situ assessment using tracers in dome-shaped incubation chambers

  • Richard L. Smith
  • John Karl Böhlke
  • Deborah A. Repert
  • Charles P. Hart
Article

Abstract

The extent to which in-stream processes alter or remove nutrient loads in agriculturally impacted streams is critically important to watershed function and the delivery of those loads to coastal waters. In this study, patch-scale rates of in-stream benthic processes were determined using large volume, open-bottom benthic incubation chambers in a nitrate-rich, first to third order stream draining an area dominated by tile-drained row-crop fields. The chambers were fitted with sampling/mixing ports, a volume compensation bladder, and porewater samplers. Incubations were conducted with added tracers (NaBr and either 15N[NO3], 15N[NO2], or 15N[NH4+]) for 24–44 h intervals and reaction rates were determined from changes in concentrations and isotopic compositions of nitrate, nitrite, ammonium and nitrogen gas. Overall, nitrate loss rates (220–3,560 μmol N m−2 h−1) greatly exceeded corresponding denitrification rates (34–212 μmol N m−2 h−1) and both of these rates were correlated with nitrate concentrations (90–1,330 μM), which could be readily manipulated with addition experiments. Chamber estimates closely matched whole-stream rates of denitrification and nitrate loss using 15N. Chamber incubations with acetylene indicated that coupled nitrification/denitrification was not a major source of N2 production at ambient nitrate concentrations (175 μM), but acetylene was not effective for assessing denitrification at higher nitrate concentrations (1,330 μM). Ammonium uptake rates greatly exceeded nitrification rates, which were relatively low even with added ammonium (3.5 μmol N m−2 h−1), though incubations with nitrite demonstrated that oxidation to nitrate exceeded reduction to nitrogen gas in the surface sediments by fivefold to tenfold. The chamber results confirmed earlier studies that denitrification was a substantial nitrate sink in this stream, but they also indicated that dissolved inorganic nitrogen (DIN) turnover rates greatly exceeded the rates of permanent nitrogen removal via denitrification.

Keywords

Denitrification Nitrification Stream bed Benthic chamber Isotope tracer 

References

  1. Alexander RB, Smith RA, Schwarz GE, Boyer EW, Nolan JV, Brakebill JW (2008) Differences in phosphorus and nitrogen delivery to the Gulf of Mexico from the Mississippi River basin. Environ Sci Technol 42:822–830CrossRefGoogle Scholar
  2. Antweiler RC, Smith RL, Voytek MA, Böhlke JK, Richards KD (2004) Water-quality data from two agricultural drainage basins in northwestern Indiana and northeastern Illinois: I. Lagrangian data, 1999–2002. U S Geol Survey Open-File Report 2004-1317, BoulderGoogle Scholar
  3. Antweiler RC, Smith RL, Voytek MA, Böhlke JK (2005a) Water-quality data from two agricultural drainage basins in northwestern Indiana and northeastern Illinois: II. Diel data, 1999–2001. U S Geol Survey Open-File Report 2005-1053, BoulderGoogle Scholar
  4. Antweiler RC, Smith RL, Voytek MA, Böhlke JK, Dupre DH (2005b) Water-quality data from two agricultural drainage basins in northwestern Indiana and northeastern Illinois: III. Biweekly data, 2000–2002. U S Geol Survey Open-File Report 2005-1197, BoulderGoogle Scholar
  5. Arah JRM, Crichton IJ, Smith KA (1993) Denitrification measured directly using a single-inlet mass spectrometer and by acetylene inhibition. Soil Biol Biochem 25:233–238CrossRefGoogle Scholar
  6. Arrigo KR (2005) Marine microorganisms and global nutrient cycles. Nature 437:349–355CrossRefGoogle Scholar
  7. Bartkow ME, Udy JW (2004) Quantifying potential nitrogen removal by denitrification in stream sediments at a regional scale. Mar Freshw Res 55:309–315CrossRefGoogle Scholar
  8. Bernot MJ, Dodds WK, Gardner WS, McCarthy MJ, Sobolev D, Tank JL (2003) Comparing denitrification estimates for a Texas estuary by using acetylene inhibition and membrane inlet mass spectrometry. Appl Environ Microbiol 69:5950–5956CrossRefGoogle Scholar
  9. Böhlke JK, Harvey JW, Voytek MA (2004) Reach-scale isotope tracer experiment to quantify denitrification and related processes in a nitrate-rich stream, mid-continent United States. Limnol Oceanogr 49:821–838Google Scholar
  10. Böhlke JK, Smith RL, Miller DN (2006) Ammonium transport and reaction in contaminated groundwater: application of isotope tracers and isotope fractionation studies. Water Resour Res 42:W05411. doi:10.1029/2005WR004349 CrossRefGoogle Scholar
  11. Böhlke JK, Smith RL, Hannon JE (2007) Isotopic analysis of N and O in nitrite and nitrate by sequential selective bacterial reduction to N2O. Anal Chem 79:5888–5895CrossRefGoogle Scholar
  12. Böhlke JK, Antweiler RC, Harvey JW, Laursen AE, Smith LK, Smith RL, Voytek MA (2009) Multiscale measurements and modeling of denitrification in streams with varying flow and nitrate concentrations in the upper Mississippi River basin, USA. Biogeochemistry 93:117–141CrossRefGoogle Scholar
  13. Bollmann A, Conrad R (1997) Acetylene blockage technique leads to underestimation of denitrification rates in oxic soils due to scavenging of intermediate nitric oxide. Soil Biol Biochem 29:1067–1077CrossRefGoogle Scholar
  14. Bott TL, Brock JT, Cushing CE, Gregory SV, King D, Petersen RC (1978) A comparison of methods for measuring primary productivity and community respiration in streams. Hydrobiologia 60:3–12CrossRefGoogle Scholar
  15. Bott TL, Brock JT, Baattrup-Pedersen A, Chambers PA, Dodds WK, Himbeault KT, Lawrence JR, Planas D, Snyder E, Wolfaardt GM (1997) An evaluation of techniques for measuring periphyton metabolism in chambers. Can J Fisheries Aquat Sci 54:715–725CrossRefGoogle Scholar
  16. Brooks MH, Smith RL, Macalady DL (1992) Inhibition of existing denitrification enzyme activity by chloramphenicol. Appl Environ Microbiol 58:1746–1753Google Scholar
  17. Casciotti KL, Sigman DM, Hastings GM, Böhlke JK, Hilkert A (2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74:4905–4912CrossRefGoogle Scholar
  18. Coplen TB, Böhlke JK, Casciotti KL (2004) Using dual bacterial denitrification to improve δ15N determinations of nitrates containing mass independent 17O. Rapid Commun Mass Spectrom 18:245–250CrossRefGoogle Scholar
  19. Dalsgaard T, Canfield DE, Petersen J, Thamdrup B, Acuña-González J (2003) N2 production by the anammox reaction in the anoxic water column of the Golf Dulce, Costa Rica. Nature 422:606–608CrossRefGoogle Scholar
  20. Duff JH, Triska FJ (2000) Nitrogen biogeochemistry and surface-subsurface exchange in streams. In: Jones JB, Mulholland PJ (eds) Streams and ground waters. Academic Press, New YorkGoogle Scholar
  21. Duff JH, Murphy F, Fuller CC, Triska FJ, Harvey JW, Jackman AP (1998) A mini drivepoint sampler for measuring pore water solute concentrations in the hyporheic zone of sand-bottom streams. Limnol Oceanogr 43:1378–1383CrossRefGoogle Scholar
  22. Duff JH, Tesoriero AJ, Richardson WB, Strauss EA, Munn MD (2008) Whole-stream response to nitrate loading in three streams draining agricultural landscapes. J Environ Qual 37:1133–1144CrossRefGoogle Scholar
  23. Fellows CS, Valett HM, Dahm CN (2001) Whole-stream metabolism in two montane streams: contribution of the hyporheic zone. Limnol Oceanogr 46:523–531Google Scholar
  24. Galloway JN, Dentener FJ, Capone DG, Boyer EW, Howarth RW, Seitzinger SP, Asner GP, Cleveland CC, Green PA, Holland EA, Karl DM, Michaels AF, Porter JH, Townsend AR, Vörösmarty CJ (2004) Nitrogen cycles: past, present and future. Biogeochemistry 70:153–226CrossRefGoogle Scholar
  25. Goolsby DA, Battaglin WA, Aulenbach BT, Hooper RP (2001) Nitrogen input to the Gulf of Mexico. J Environ Qual 30:329–336Google Scholar
  26. Grimm NB, Sheibley RW, Crenshaw CL, Dahm CN, Roach WJ, Zeglin LH (2005) N retention and transformation in urban streams. J North Am Benthol Soc 24:626–642Google Scholar
  27. Groffman PM, Dorsey AM, Mayer PM (2005) N processing within geomorphic structures in urban streams. J North Am Benthol Soc 24:613–625Google Scholar
  28. Holmes RM, Jones JB Jr, Fisher SG, Grimm NB (1996) Denitrification in a nitrogen-limited stream ecosystem. Biogeochemistry 33:125–146CrossRefGoogle Scholar
  29. Jenkins MC, Kemp WM (1984) The coupling of nitrification and denitrification in two estuarine sediments. Limnol Oceanogr 29:609–619CrossRefGoogle Scholar
  30. Joye SB, Smith SV, Hollibaugh JT, Paerl HW (1996) Estimating denitrification rates in estuarine sediments: a comparison of stoichiometric and acetylene based methods. Biogeochemistry 33:197–215CrossRefGoogle Scholar
  31. Kemp WM, Sampou P, Caffrey J, Mayer M, Henriksen K, Boynton WR (1990) Ammonium recycling versus denitrification in Chesapeake Bay sediments. Limnol Oceanogr 35:1545–1563CrossRefGoogle Scholar
  32. Knowles R (1982) Denitrification. Microbiol Rev 46:43–70Google Scholar
  33. Knowles R (1990) Acetylene inhibition technique: development, advantages, and potential problems. In: Revsbech NP, Sorensen J (eds) Denitrification in soil and sediment. Plenum Press, New YorkGoogle Scholar
  34. Laursen AE, Seitzinger S (2002) Measurement of denitrification in rivers: an integrated whole reach approach. Hydrobiologia 485:67–81CrossRefGoogle Scholar
  35. Mosier AR, Guenzi WD, Schweizer EE (1986) Field denitrification estimation by nitrogen-15 and acetylene inhibition techniques. Soil Sci Soc Am J 50:831–833CrossRefGoogle Scholar
  36. Mulholland PJ, Valett HM, Webster JR, Thomas SA, Cooper LW, Hamilton SK (2004) Stream denitrification and total nitrate uptake rates measured using a field 15N tracer addition approach. Limnol Oceanogr 49:809–820CrossRefGoogle Scholar
  37. Mulholland PJ, Helton AM, Poole GC, Hall RO Jr, Hamilton SK, Peterson BJ, Tank JL, Ashkenas LR, Cooper LW, Dahm CN, Dodds WK, Findlay SEG, Gregory SV, Grimm NB, Johnson SL, McDowell WH, Meyer JL, Valett HM, Webster JR, Arango CP, Beaulieu JJ, Bernot MJ, Burgin AJ, Crenshaw CL, Johnson LT, Neiderlehner BR, O’Brien JM, Potter JD, Sheibley RW, Sobota DJ, Thomas SM (2008) Stream denitrification across biomes and its response to anthropogenic nitrate loading. Nature 452:202–206CrossRefGoogle Scholar
  38. Oremland RS, Umberger C, Culbertson CW, Smith RL (1984) Denitrification in San Francisco Bay intertidal sediments. Appl Environ Microbiol 47:1106–1112Google Scholar
  39. Peterson BJ, Wolheim WM, Mulholland PJ, Webster JR, Meyer JL, Tank JL, Marti E, Bowden WB, Valett HM, Hershey AE, McDowell WH, Dodds WK, Hamilton SK, Gregory S, Morrall DD (2001) Control of nitrogen export from watersheds by headwater streams. Science 292:86–90CrossRefGoogle Scholar
  40. Pribyl AL, McCutchan JH, Lewis WM Jr, Saunders JF (2005) Whole-system estimation of denitrification in a plains river: a comparison of two methods. Biogeochemistry 73:439–455CrossRefGoogle Scholar
  41. Rabalais NN, Wiseman WJ Jr, Turner RE (1994) Comparison of continuous records of near-bottom dissolved oxygen from the hypoxia zone along the Louisiana coast. Estuaries 14:850–861CrossRefGoogle Scholar
  42. Scavia D, Rabalais NN, Turner RE, Justic D, Wiseman WJ Jr (2003) Predicting the response of Gulf of Mexico hypoxia to variations in Mississippi River nitrogen load. Limnol Oceanogr 48:951–956CrossRefGoogle Scholar
  43. Schade JD, Welter JR, Marti E, Grimm NB (2005) Hydrologic exchange and N uptake by riparian vegetation in an arid-land stream. J North Am Benthol Soc 24:19–28CrossRefGoogle Scholar
  44. Schaller JL, Royer TV, David MB (2004) Denitrification associated with plants and sediments in an agricultural stream. J North Am Benthol Soc 23:667–676CrossRefGoogle Scholar
  45. Schlesinger WH, Reckhow KH, Bernhardt ES (2006) Global change: the nitrogen cycle in rivers. Water Resour Res 42:W03S06. doi:10.1029/2005WR004300 CrossRefGoogle Scholar
  46. Seitzinger SP (1988) Denitrification in freshwater and coastal marine ecosystems: ecological and geochemical significance. Limnol Oceanogr 33:702–724CrossRefGoogle Scholar
  47. Seitzinger SP, Nielsen LP, Caffrey J, Christensen PB (1993) Denitrification measurements in aquatic sediments: a comparison of three methods. Biogeochemistry 23:147–167CrossRefGoogle Scholar
  48. Sheibley RW, Duff JH, Jackman AP, Triska FJ (2003) Inorganic nitrogen transformations in the bed of the Shingobee River, Minnesota: integrating hydrologic and biological processes using sediment perfusion cores. Limnol Oceanogr 48:1129–1140CrossRefGoogle Scholar
  49. Sigman DM, Casciotti KL, Andreani M, Barford C, Galanter M, Böhlke JK (2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73:4145–4153CrossRefGoogle Scholar
  50. Smith RL, Miller LG, Howes BL (1993) The geochemistry of methane in Lake Fryxell, an amictic, permanently ice-covered, antarctic lake. Biogeochemistry 21:95–115CrossRefGoogle Scholar
  51. Smith RL, Garabedian SP, Brooks MH (1996) Comparison of denitrification activity measurements in groundwater using cores and natural-gradient tracer tests. Environ Sci Technol 30:3448–3456CrossRefGoogle Scholar
  52. Smith RL, Böhlke JK, Garabedian SP, Revesz KM, Yoshinari T (2004) Assessing denitrification in groundwater using natural gradient tracer tests with 15N: In situ measurement of a sequential multistep reaction. Water Resour Res 40:W07101. doi:10.1029/2003WR002019 CrossRefGoogle Scholar
  53. Smith RL, Buckwalter SP, Repert DA (2005) Small-scale, hydrogen-oxidizing-denitrifying bioreactor for treatment of nitrate-contaminated drinking water. Water Res 39:2014–2023CrossRefGoogle Scholar
  54. Smith LK, Voytek MA, Böhlke JK, Harvey JW (2006a) Denitrification in nitrate-rich streams: application of N2:Ar and 15N-tracer methods in intact cores. Ecol Appl 16:2191–2207CrossRefGoogle Scholar
  55. Smith RL, Baumgartner LK, Miller DN, Repert DA, Böhlke JK (2006b) Assessment of nitrification potential in ground water using short term, single-well injection experiments. Microb Ecol 51:22–35CrossRefGoogle Scholar
  56. Starry OS, Valett HM, Schreiber ME (2005) Nitrification rates in a headwater stream: influences of seasonal variation in C and N supply. J North Am Benthol Soc 24:753–768CrossRefGoogle Scholar
  57. Tank JL, Meyer JL, Sanzone DM, Mulholland PJ, Webster JR, Peterson BJ, Wollheim WM, Leonard NE (2000) Analysis of nitrogen cycling in a forest stream during autumn using a 15N-tracer addition. Limnol Oceanogr 45:1013–1029CrossRefGoogle Scholar
  58. Trimmer M, Nicholls JC, Deflandre B (2003) Anaerobic ammonium oxidation measured in sediments along the Thames Estuary, United Kingdom. Appl Environ Microbiol 69:6447–6454CrossRefGoogle Scholar
  59. Trimmer M, Nicholls JC, Morley N, Davies CA, Aldridge J (2005) Biphasic behavior of anammox regulated by nitrite and nitrate in an estuarine sediment. Appl Environ Microbiol 71:1923–1930CrossRefGoogle Scholar
  60. USGS (2008) Stream-flow and water quality data. U S geological survey, national water information system. http://waterdata.usgs.gov/nwis, Reston
  61. Uzarski DG, Burton TM, Stricker CA (2001) A new chamber design for measuring community metabolism in a Michigan stream. Hydrobiologia 455:137–155CrossRefGoogle Scholar
  62. Uzarski DG, Stricker CA, Burton TM, King DK, Steinman AD (2004) The importance of hyporheic sediment respiration in several mid-order Michigan rivers: comparison between methods in estimates of lotic metabolism. Hydrobiologia 518:47–57CrossRefGoogle Scholar
  63. Vidon P, Hill AR (2004) Denitrification and patterns of electron donors and acceptors in eight riparian zones with contrasting hydrogeology. Biogeochemistry 71:259–283CrossRefGoogle Scholar
  64. Watts SH, Seitzinger SP (2000) Denitrification rates in organic and mineral soils from riparian sites: a comparison of N2 flux and acetylene inhibition methods. Soil Biol Biochem 32:1383–1392CrossRefGoogle Scholar
  65. Wollheim WM, Peterson BJ, Deegan LA, Hobbie JE, Hooker B, Bowden WB, Edwardson KJ, Arscott DB, Hershey AE, Finlay J (2001) Influence of stream size on ammonium and suspended particulate nitrogen processing. Limnol Oceanogr 46:1–13CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Richard L. Smith
    • 1
  • John Karl Böhlke
    • 2
  • Deborah A. Repert
    • 1
  • Charles P. Hart
    • 1
  1. 1.US Geological SurveyBoulderUSA
  2. 2.US Geological SurveyRestonUSA

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