Abstract
Triadimefon, a type of triazole systemic fungicide, has been extensively used to control various fungal diseases. However, triadimefon could lead to severe environmental pollution, and even threatens human health. To eliminate triadimefon residues, a triadimefon-degrading bacterial strain TY18 was isolated from a long-term polluted site and was identified as Enterobacter hormaechei. Strain TY18 could grow well in a carbon salt medium with triadimefon as the sole nitrogen source, and could efficiently degrade triadimefon. Under triadimefon stress, a total of 430 differentially expressed genes (DEGs), including 197 up-regulated and 233 down-regulated DEGs, were identified in strain TY18 using transcriptome sequencing (RNA-Seq). Functional classification and enrichment analysis revealed that these DEGs were mainly related to amino acid transport and metabolism, carbohydrate transport and metabolism, small molecule and pyrimidine metabolism. Interestingly, the DEGs encoding monooxygenase and hydrolase activity acting on carbon–nitrogen were highly up-regulated, might be mainly responsible for the metabolism in triadimefon. Our findings in this work suggest that strain E. hormaechei TY18 could efficiently degrade triadimefon for the first time. They provide a great potential to manage triadimefon biodegradation in the environment successfully.
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Data availability
The raw RNA-seq data were deposited in NCBI Sequence Read Archive database under BioProject accession number PRJNA923466.
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References
Chen TX, Chen XR, Wang Y, Xu PG, Yang XL (2013) Pathogen identification and chemical control of powdery mildew on Astragalus membranaceus. Agrochemicals 52(8):599–601. https://doi.org/10.16820/j.cnki.1006-0413.2013.08.017
Cheng Y, Zang H, Wang H, Li D, Li C (2018) Global transcriptomic analysis of Rhodococcus erythropolis D310–1 in responding to chlorimuron-ethyl. Ecotox Environ Safe 157:111–120. https://doi.org/10.1016/j.ecoenv.2018.03.074
Dickens JSW (2007) Studies on the chemical control of chrysanthemum white rust caused by Puccinia horiana. Plant Pathol 39(3):434–442. https://doi.org/10.1111/j.1365-3059.1990.tb02519.x
Ganeshan G, Chethana BS, Rao AS, Bellishree K (2011) Comparative efficacy of myclobutanil, triadimefon and mancozeb against major fungal diseases of chilli. Pest Manag Hortic Ecosyst 17(1):42–47
Holt S, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Lippincott Williams and Wilkins, Baltimore
Kong Z, Quan R, Fan B, Liao Y, Chen J, Li M, Dai X (2020) Stereoselective behaviors of the fungicide triadimefon and its metabolite triadimenol during malt storage and beer brewing. J Hazard Mater 400:123238. https://doi.org/10.1016/j.jhazmat.2020.123238
Li Y, Dong F, Liu X, Xu J, Han Y, Zheng Y (2014) Chiral fungicide triadimefon and triadimenol: stereoselective transformation in greenhouse crops and soil, and toxicity to Daphnia magna. J Hazard Mater 265:115–123. https://doi.org/10.1016/j.jhazmat.2013.11.055
Liu N, Jin X, Zhou J, Wang Y, Yang Q, Wu F, Giesy JP, Johnson AC (2018) Predicted no-effect concentration (PNEC) and assessment of risk for the fungicide, triadimefon based on reproductive fitness of aquatic organisms. Chemosphere 207:682–689. https://doi.org/10.1016/j.chemosphere.2018.05.093
Liu Y, Hu H, Zanaroli G, Xu P, Tang H (2021) A Pseudomonas sp. strain uniquely degrades PAHs and heterocyclic derivatives via lateral dioxygenation pathways. J Hazard Mater 403:123956. https://doi.org/10.1016/j.jhazmat.2020.123956
Ma Y, Li B, Ke Y, Zhang Y, Zhang Y (2018) Transcriptome analysis of Rana chensinensis liver under trichlorfon stress. Ecotox Environ Safe 147:487–493. https://doi.org/10.1016/j.ecoenv.2017.09.016
Mai B, Fan J, Jiang Y, He R, Lai Y, Zhang W (2019) Fast enantioselective determination of triadimefon in different matrices by supercritical fluid chromatography. J Chromatogr B 1126–1127:121740. https://doi.org/10.1016/j.jchromb.2019.121740
Margesin R, Volgger G, Wagner AO, Zhang D, Poyntner C (2021) Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil. Appl Microbiol Biotechnol 105(4):2967–2977. https://doi.org/10.1007/s00253-021-11215-z
Meng P, Pei H, Hu W, Shao Y, Li Z (2014) How to increase microbial degradation in constructed wetlands: influencing factors and improvement measures. Bioresour Technol 157:316–326. https://doi.org/10.1016/j.biortech.2014.01.095
Meng X, Dong F, Qian K, Miao L, Yang X, Ge H, Wu Z, Wang J (2019) Transcriptome analysis reveals global gene expression changes of Chilo suppressalis in response to sublethal dose of chlorantraniliprole. Chemosphere 234:648–657. https://doi.org/10.1016/j.chemosphere.2019.06.129
Olotu MI, Maniania NK, Ekesi S, Seguni ZS, du Plessis H (2013) Effect of fungicides used for powdery mildew disease management on the African weaver ant, Oecophylla longinoda (Hymenoptera: Formicidae), a biocontrol agent of sap-sucking pests in cashew crops in Tanzania. Int J Trop Insect Sci 33(4):283–290. https://doi.org/10.1017/S1742758413000313
Peng F, Ye M, Liu Y, Liu J, Lan Y, Luo A, Zhang T, Jiang SH (2022) Comparative genomics reveals response of Rhodococcus pyridinivorans B403 to phenol after evolution. Appl Microbiol Biotechnol 106(7):2751–2761. https://doi.org/10.1007/s00253-022-11858-6
Reddy KSN, Hisanaga T, Tanaka K (1999) Photodegradation of fungicide triadimefon and pesticide pirimicarb in aqueous TIO2 suspension. Toxicol Environ Chem 68(3–4):403–412. https://doi.org/10.1080/02772249909358673
Satapute P, Kaliwal B (2016) Biodegradation of the fungicide propiconazole by Pseudomonas aeruginosa PS-4 strain isolated from a paddy soil. Ann Microbiol 66(4):1355–1365. https://doi.org/10.1007/s13213-016-1222-6
Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C (T) method. Nat Protoc 3:1101–1108. https://doi.org/10.1038/nprot.2008.73%3e
Shang X, Geng L, Yang J, Zhang Y, Xu W (2021) Transcriptome analysis reveals the mechanism of alkalinity exposure on spleen oxidative stress, inflammation and immune function of Luciobarbus capito. Ecotox Environ Safe 225:112748. https://doi.org/10.1016/j.ecoenv.2021.112748
Singh N (2005) Factors affecting triadimefon degradation in soils. J Agr Food Chem 53(1):70–75. https://doi.org/10.1021/jf048884j
Singh A, Pandey AK, Dubey SK (2021) Biodegradation of isoprene by Arthrobacter sp. strain BHU FT2: genomics-proteomics enabled novel insights. Bioresour Technol 340(3):125634. https://doi.org/10.1016/j.biortech.2021.125634
Sun X, Yang G, Cao X, Lin Z (2008) Photochemical degradation of triadimefon in seawater. J Ocean U Chin 7(2):171–176. https://doi.org/10.1007/s11802-008-0171-5
Talab KMA, Yang ZH, Li JH, Zhao Y, Xiong YB (2018) The influence of microbial communities for triadimefon enantiomerization in soils with different pH values. Chirality 30(11):293–301. https://doi.org/10.1002/chir.22796
Tang S, Yin H, Yu X, Chen S, Dang Z (2021) Transcriptome profiling of Pseudomonas aeruginosa YH reveals mechanisms of 2, 2’, 4, 4’-tetrabrominated diphenyl ether tolerance and biotransformation. J Hazard Mater 403:12408. https://doi.org/10.1016/j.jhazmat.2020.124038
Trivedi VD, Jangir PK, Sharma R, Phale PS (2016) Insights into functional and evolutionary analysis of carbaryl metabolic pathway from Pseudomonas sp. strain C5 pp. Sci Rep 6:38430. https://doi.org/10.1038/srep38430
U.S. Environmental Protection Agency (U.S. EPA) (2006) Triadimefon reregistration eligibility decision (RED) and triadimenol tolerance reassessment and risk management decision (TRED) fact sheet. Offifice of Pesticide Programs, U.S. Environmental Protection Agency, Washington
Vera A, Wilson FP, Cupples AM (2022) Predicted functional genes for the biodegradation of xenobiotics in groundwater and sediment at two contaminated naval sites. Appl Microbiol Biotechnol 106(2):835–853. https://doi.org/10.1007/s00253-021-11756-3
Wang Y, Wang C, Li A, Gao J (2015) Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19. Lett Appl Microbiol 61(4):403–410. https://doi.org/10.1111/lam.12476
Wang X, Hou X, Liang S, Lu Z, Hou Z, Zhao X, Sun F, Zhang H (2018) Biodegradation of fungicide Tebuconazole by Serratia marcescens strain B1 and its application in bioremediation of contaminated soil. Int Biodeter Biodegr 127:185–191. https://doi.org/10.1016/j.ibiod.2017.12.001
Wang Y, Zhang X, Wang L, Wang C, Fan W, Wang J (2019) Effective biodegradation of pentachloronitrobenzene by a novel strain Peudomonas putida QTH3 isolated from contaminated soil. Ecotox Environ Safe 182:109463. https://doi.org/10.1016/j.ecoenv.2019.109463
Wang X, Lu H, Li Q, Zhou Y, Zhou J (2022) Comparative genome and transcriptome of Rhodococcus pyridinivorans GF3 for analyzing the detoxification mechanism of anthraquinone compounds. Ecotox Environ Safe 237:113545. https://doi.org/10.1016/j.ecoenv.2022.113545
Woo C, Daniels B, Stirling R, Morris P (2010) Tebuconazole and propiconazole tolerance and possible degradation by basidiomycetes: a wood-based bioassay. Int Biodeter Biodegr 64(5):403–408. https://doi.org/10.1016/j.ibiod.2010.01.009
Wu Z, Liu Y, Zhang J, Shen W, Lu W, Hong Q, Li S (2011) Isolation of a heavy metal-resistant 4-chloronitrobenzene degrader Cupriavidus sp. D4 and cloning of its cnb genes. Int Biodeter Biodegr 65(6):871–876. https://doi.org/10.1016/j.ibiod.2011.06.007
Wu H, Shen J, Jiang X, Liu X, Sun X, Li J, Han W, Mu Y, Wang L (2018) Bioaugmentation potential of a newly isolated strain Sphingomonas sp. NJUST37 for the treatment of wastewater containing highly toxic and recalcitrant tricyclazole. Bioresour Technol 264:98–105. https://doi.org/10.1016/j.biortech.2018.05.071
Yamamoto S, Harayama S (1995) PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microb 61(3):1104–1109. https://doi.org/10.1016/j.ijms.2010.08.029
Yang ZK, Huang XL, Peng L (2022) Transcriptome analysis reveals gene expression changes of the basidiomycetous yeast Apiotrichum mycotoxinivorans in response to ochratoxin A exposure. Ecotox Environ Safe 246:114146. https://doi.org/10.1016/j.ecoenv.2022.114146
Zhang C, Jia L, Wang S, Qu J, Li K, Xu L, Shi Y, Yan Y (2010) Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity. Bioresour Technol 101(10):3423–3429. https://doi.org/10.1016/j.biortech.2009.12.083
Zhang X, Xu D, Zhu C, Lundaa T, Scherr KE (2012) Isolation and identification of biosurfactant producing and crude oil degrading Pseudomonas aeruginosa strains. Chem Eng J 209:138–146. https://doi.org/10.1016/j.cej.2012.07.110
Zhang H, Zhang Y, Hou Z, Wang X, Wang J, Lu Z, Zhao X, Sun F, Pan H (2016) Biodegradation potential of deltamethrin by the Bacillus cereus strain Y1 in both culture and contaminated soil. Int Biodeter Biodegr 106:53–59. https://doi.org/10.1016/j.ibiod.2015.10.005
Zhang W, Lu Y, Huang L, Cheng C, Di S, Chen L, Zhou Z, Diao J (2018) Comparison of triadimefon and its metabolite on acute toxicity and chronic effects during the early development of Rana nigromaculata tadpoles. Ecotox Environ Safe 156:247–254. https://doi.org/10.1016/j.ecoenv.2018.03.009
Zhang M, Ren Y, Du S, Zhou Y, Jiang W, Ke Z, Jiang M, Qiu J, He J, Hong Q (2021) A novel hydrolase PyzH catalyses the cleavage of C=N double bond for pymetrozine degradation in Pseudomonas sp. BYT-1. Environ Microbiol 23(6):3265–3273. https://doi.org/10.1111/1462-2920.15557
Zhang L, Yang Z, Yang M, Yang F, Wang G, Liu D, Li X, Yang L, Wang Z (2022) Copper-induced oxidative stress, transcriptome changes, intestinal microbiota, and histopathology of common carp (Cyprinus carpio). Ecotox Environ Safe 246:114136. https://doi.org/10.1016/j.ecoenv.2022.114136
Zhou Z, Wu X, Lin Z, Pang S, Mishra S, Chen S (2021) Biodegradation of fipronil: current state of mechanisms of biodegradation and future perspectives. Appl Microbiol Biotechnol 105(20):7695–7708. https://doi.org/10.1007/s00253-021-11605-3
Acknowledgements
This work was supported by Basic Research Program Project of Shanxi Province (20210302123405), Scientific Research cultivation and Innovation Project of Plant Protection College, Shanxi Agricultural University (ZBXY23B-3), Science and Technology Innovation Projects of Universities in Shanxi Province (2021L102), the Earmarked Fund for Modern Agro-industry Technology Research System (2021-11).
Funding
Funding was provided by Scientific Research cultivation and Innovation Project of Plant Protection College, Shanxi Agricultural Universit (Granty No. ZBXY23B-3), Basic Research Program Project of Shanxi Province (Grant No. 20210302123405), Science and Technology Innovation Projects of Universities in Shanxi Province (Grant No. 2021L102), the Earmarked Fund for Modern Agro-industry Technology Research System (Grant No. 2021-11).
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YW conceived and planned this work, drafted the manuscript. QG, JW and XZ contributed to the transcriptome sequencing. JL and WF performed the detection method. RZ analyzed the experimental results. CW supervised the experiments, and revised the manuscript. All authors read and approved the manuscript.
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Wang, Y., Guan, Q., Jiao, W. et al. Isolation, identification and transcriptome analysis of triadimefon-degrading strain Enterobacter hormaechei TY18. Biodegradation (2024). https://doi.org/10.1007/s10532-024-10076-3
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DOI: https://doi.org/10.1007/s10532-024-10076-3