Abstract
Acetaldehyde (AL), a primary carcinogen, not only pollutes the environment, but also endangers human health after drinking alcohol. Here a promising bacterial strain was successfully isolated from a white wine cellar pool in the province of Shandong, China, and identified as Bacillus velezensis-YW01 with 16 S rDNA sequence. Using AL as sole carbon source, initial AL of 1 g/L could be completely biodegraded by YW01 within 84 h and the cell-free extracts of YW01 has also been detected to biodegrade the AL, which indicate that YW01 is a high-potential strain for the biodegradation of AL. The optimal culture conditions and the biodegradation of AL of YW01 are at pH 7.0 and 38 °C, respectively. To further analyze the biodegradation mechanism of AL, the whole genome of YW01 was sequenced. Genes ORF1040, ORF1814 and ORF0127 were revealed in KEGG, which encode for acetaldehyde dehydrogenase. Furthermore, ORF0881 and ORF052 encode for ethanol dehydrogenase. This work provides valuable information for exploring metabolic pathway of converting ethanol to AL and subsequently converting AL to carboxylic acid compounds, which opened up potential pathways for the development of microbial catalyst against AL.
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References
Al-Shamma SA, Zaher DM, Hersi F, Abu Jayab NN, Omar HA (2023) Targeting aldehyde dehydrogenase enzymes in combination with chemotherapy and immunotherapy: an approach to tackle resistance in cancer cells. Life Sci. https://doi.org/10.1016/j.lfs.2023.121541
Borah T, Gogoi B, Khataniar A, Gogoi M, Das A, Borah D (2019) Probiotic characterization of indigenous Bacillus velezensis strain DU14 isolated from Apong, a traditionally fermented rice beer of Assam. Biocatal Agric Biotechnol 18:101008. https://doi.org/10.1016/j.bcab.2019.01.046
Brooks PJ, Enoch MA, Goldman D, Li TK, Yokoyama A (2009) The alcohol flushing response: an unrecognized risk factor for esophageal cancer from alcohol consumption. PLoS Med. https://doi.org/10.1371/journal.pmed.1000050
Chang S, Li B, Chen T, Zhang L, Li Y, He X et al (2023) Engineering aldehyde dehydrogenase PaALDH70140 from Pseudomonas aeruginosa PC-1 with improved catalytic properties for 5-hydroxymethyl-2-furancarboxylic acid synthesis. Biochem Eng J. https://doi.org/10.1016/j.bej.2023.108835
Chen CH, Ferreira JCB, Joshi AU, Stevens MC, Li SJ, Hsu JH et al (2020) Novel and prevalent non-east Asian ALDH2 variants; implications for global susceptibility to aldehydes’ toxicity. EBioMedicine 55:102753. https://doi.org/10.1016/j.ebiom.2020.102753
Chun BH, Kim KH, Jeong SE, Jeon CO (2019) Genomic and metabolic features of the Bacillus amyloliquefaciens group- B. amyloliquefaciens, B. velezensis, and B. siamensis- revealed by pan-genome analysis. Food Microbiol. https://doi.org/10.1016/j.fm.2018.09.001
Delikhoon M, Fazlzadeh M, Sorooshian A, Baghani AN, Golaki M, Ashournejad Q et al (2018) Characteristics and health effects of formaldehyde and acetaldehyde in an urban area in Iran. Environ Pollut 242(Pt A):938–951. https://doi.org/10.1016/j.envpol.2018.07.037
Dhanya Raj CT, Suryavanshi MV, Kandaswamy S, Ramasamy KP, James RA (2023) Whole genome sequence analysis and in-vitro probiotic characterization of Bacillus velezensis FCW2 MCC4686 from spontaneously fermented coconut water. Genomics 115(4):110637. https://doi.org/10.1016/j.ygeno.2023.110637
Duryee MJ, Aripova N, Hunter CD, Ruskamp RJ, Tessin MR, Works DR et al (2022) A novel reactive aldehyde species inhibitor prevents the deleterious effects of ethanol in an animal model of alcoholic liver disease. Int Immunopharmacol 113(Pt A):109400. https://doi.org/10.1016/j.intimp.2022.109400
Etesami H, Jeong BR, Glick BR (2023) Potential use of Bacillus spp. as an effective biostimulant against abiotic stresses in crops—a review. Curr Res Biotechnol. https://doi.org/10.1016/j.crbiot.2023.100128
Felczak MM, TerAvest MA. 2022. Zymomonas mobilis ZM4 Utilizes an NADP+-Dependent Acetaldehyde Dehydrogenase To Produce Acetate. J Bacteriol 204:e00563-21. https://doi.org/10.1128/jb.00563-21
Ge Z, Kuang Z, Chen J, Chen J, Liu T, She Z et al (2022) Comparative genomics analysis of Bacillus velezensis LOH112 isolated from a nonagenarian provides insights into its biocontrol and probiotic traits. Gene 835:146644. https://doi.org/10.1016/j.gene.2022.146644
Guan X, Rubin E, Anni H (2012) An optimized method for the measurement of acetaldehyde by high-performance liquid chromatography. Alcohol Clin Exp Res 36(3):398–405. https://doi.org/10.1111/j.1530-0277.2011.01612.x
Gupta J, Rathour R, Dupont CL, Kaul D, Thakur IS (2021) Genomic insights into waste valorized extracellular polymeric substances (EPS) produced by Bacillus sp. ISTL8. Environ Res 192:110277. https://doi.org/10.1016/j.envres.2020.110277
Hadei M, Shahsavani A, Hopke PK, Kermani M, Yarahmadi M, Mahmoudi B (2019) Comparative health risk assessment of in-vehicle exposure to formaldehyde and acetaldehyde for taxi drivers and passengers: effects of zone, fuel, refueling, vehicle’s age and model. Environ Pollut. https://doi.org/10.1016/j.envpol.2019.07.111
He G, Dong Y, Huang J, Wang X, Zhang S, Wu C et al (2019) Alteration of microbial community for improving flavor character of Daqu by inoculation with Bacillus velezensis and Bacillus subtilis. Lwt 111:1–8. https://doi.org/10.1016/j.lwt.2019.04.098
Hwangbo K, Um Y, Kim KY, Madhaiyan M, Sa TM, Lee Y (2016) Complete genome sequence of Bacillus velezensis CBMB205, a phosphate-solubilizing bacterium isolated from the rhizoplane of rice in the Republic of Korea. Genome Announcements. https://doi.org/10.1128/genomeA.00654-16
Jakinala P, Lingampally N, Kyama A, Hameeda B (2019) Enhancement of atrazine biodegradation by marine isolate Bacillus velezensis MHNK1 in presence of surfactin lipopeptide. Ecotoxicol Environ Saf 182:109372. https://doi.org/10.1016/j.ecoenv.2019.109372
Jung SJ, Hwang JH, Park EO, Lee SO, Chung YJ, Chung MJ et al (2021) Regulation of alcohol and acetaldehyde metabolism by a mixture of Lactobacillus and Bifidobacterium species in human. Nutrients. https://doi.org/10.3390/nu13061875
Karn SK, Chakrabarty SK, Reddy MS (2010) Characterization of pentachlorophenol degrading strains from secondary pulp-and-paper-industry sludge. Int Biodeterior Biodegrad 64(7):609–613. https://doi.org/10.1016/j.ibiod.2010.05.017
Karn SK, Chakrabarti SK, Reddy MS (2011) Degradation of pentachlorophenol by sp. CL2 isolated from secondary sludge of pulp and paper mill. Biodegradation 22(1):63–69. https://doi.org/10.1007/s10532-010-9376-6
Kawai T, Mihara T, Kawashima I, Fujita Y, Ikeda C, Negishi H et al (2012) Endogenous acetaldehyde toxicity during antral follicular development in the mouse ovary. Reprod Toxicol 33(3):322–330. https://doi.org/10.1016/j.reprotox.2012.01.001
Kim JH, Kim HJ, Son JH, Chun HN, Kim SK (2003) Effect of Lactobacillus fermentum MG590 on alcohol metabolism and liver function in rats. J Microbiol Biotechnol 13(6):919–925
Kokkinakis M, Tsakiris I, Tzatzarakis M, Vakonaki E, Alegakis A, Papachristou S et al (2020) Carcinogenic, ethanol, acetaldehyde and noncarcinogenic higher alcohols, esters, and methanol compounds found in traditional alcoholic beverages. A risk assessment approach. Toxicol Rep 7:1057–1065. https://doi.org/10.1016/j.toxrep.2020.08.017
Kosmider L, Cox S, Zaciera M, Kurek J, Goniewicz ML, McRobbie H et al (2020) Daily exposure to formaldehyde and acetaldehyde and potential health risk associated with use of high and low nicotine e-liquid concentrations. Sci Rep 10(1):6546. https://doi.org/10.1038/s41598-020-63292-1
Lee S, Kim M, Ahn BJ, Jang Y (2023) Odorant-responsive biological receptors and electronic noses for volatile organic compounds with aldehyde for human health and diseases: a perspective review. J Hazard Mater 455:131555. https://doi.org/10.1016/j.jhazmat.2023.131555
Leyrer-Jackson JM, Hood LE, Olive MF (2021) Alcohol consumption preferentially activates a subset of pro-opiomelanocortin (POMC) producing neurons targeting the amygdala. Neuropharmacology 195:108674. https://doi.org/10.1016/j.neuropharm.2021.108674
Li Y, Lei L, Zheng L, Xiao X, Tang H, Luo C (2020a) Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots. Biotechnol Biofuels. https://doi.org/10.1186/s13068-020-1671-9
Li Y, Zhao M, Chen W, Du H, Xie X, Wang D et al (2020b) Comparative transcriptomic analysis reveals that multiple hormone signal transduction and carbohydrate metabolic pathways are affected by Bacillus cereus in Nicotiana tabacum. Genomics 112(6):4254–4267. https://doi.org/10.1016/j.ygeno.2020.07.022
Liang B, Liu Y, Zhao Y, Xia T, Chen R, Yang J (2021) Development of bacterial biosensor for sensitive and selective detection of acetaldehyde. Biosens Bioelectron. https://doi.org/10.1016/j.bios.2021.113566
Liu Y, Shang X, Zeng R (2015) Permanent draft genome of acetaldehyde degradation bacterium, Shewanella sp. YQH10. Mar Genom 19:9–11. https://doi.org/10.1016/j.margen.2014.11.003
Liu X, Zhang Y, Sun Q, Liu Z, Zhao Y, Fan A et al (2022) Rapid colonization and biodegradation of untreated commercial polyethylene wrap by a new strain of Bacillus velezensis C5. J Environ Manage. https://doi.org/10.1016/j.jenvman.2021.113848
Liu D, Han Z, Hu Z, Yu C, Wang Y, Tong J et al (2023) Comparative analysis of the transcriptome of Bacillus subtilis natto incubated in different substrates for nattokinase production. Process Biochem 129:30–43. https://doi.org/10.1016/j.procbio.2023.03.005
Lu J, Zhu X, Zhang C, Lu F, Lu Z, Lu Y (2020) Co-expression of alcohol dehydrogenase and aldehyde dehydrogenase in Bacillus subtilis for alcohol detoxification. Food Chem Toxicol. https://doi.org/10.1016/j.fct.2019.110890
Lu J, Zhao Y, Cheng Y, Hu R, Fang Y, Lyu M et al (2022) Optimal secretory expression of acetaldehyde dehydrogenase from Issatchenkia terricola in Bacillus subtilis through a combined strategy. Molecules. https://doi.org/10.3390/molecules27030747
Matsuzaki K, Kumatoriya K, Tando M, Kometani T, Shinohara M (2022) Polyphenols from persimmon fruit attenuate acetaldehyde-induced DNA double-strand breaks by scavenging acetaldehyde. Sci Rep 12(1):10300. https://doi.org/10.1038/s41598-022-14374-9
Milagre CDF, Milagre HMS (2022) Alcohol dehydrogenase-catalyzed oxidation. Curr Opin Green Sustainable Chem. https://doi.org/10.1016/j.cogsc.2022.100694
Nosova T, Jousimies-Somer H, Jokelainen K, Heine R, Salaspuro M (2000) Acetaldehyde production and metabolism by human indigenous and probiotic Lactobacillus and Bifidobacterium strains. Alcohol Alcohol 35(6):561–568. https://doi.org/10.1093/alcalc/35.6.561
Olson BJSC, Markwell J (2007) Assays for determination of protein concentration. Curr Protocols Protein Sci. https://doi.org/10.1002/cpph.3
Osborne JP, de Orduña M, Pilone R, G. J., Liu SQ (2000) Acetaldehyde metabolism by wine lactic acid bacteria. FEMS Microbiol Lett 191(1):51–55. https://doi.org/10.1111/j.1574-6968.2000.tb09318.x
Peng Z, Wang R, Xia X, Zhang J (2023) Engineered acetaldehyde dehydrogenase for the efficient degradation of acetaldehyde. J Environ Manage 331:117258. https://doi.org/10.1016/j.jenvman.2023.117258
Pereira WR, Ferreira JCB, Artioli GG (2023) Commentary: aldehyde dehydrogenase, redox balance and exercise physiology: what is missing? Comp Biochem Physiol Mol Integr Physiol 283:111470. https://doi.org/10.1016/j.cbpa.2023.111470
Possanzini M, Di Palo V, Cecinato A (2002) Sources and photodecomposition of formaldehyde and acetaldehyde in Rome ambient air. Atmos Environ 36(19):3195–3201
Ratna A, Mandrekar P (2017) Alcohol and cancer: mechanisms and therapies. Biomolecules. https://doi.org/10.3390/biom7030061
Reimers MJ, Flockton AR, Tanguay RL (2004) Ethanol- and acetaldehyde-mediated developmental toxicity in zebrafish. Neurotoxicol Teratol 26(6):769–781. https://doi.org/10.1016/j.ntt.2004.06.012
Ruiz-García C, Béjar V, Martínez-Checa F, Llamas I, Quesada E (2005) Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. Int J Syst Evol MicroBiol 55(1):191–195. https://doi.org/10.1099/ijs.0.63310-0
Sam-on MFS, Mustafa S, Mohd Hashim A, Yusof MT, Zulkifly S, Malek AZA et al (2023) Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens. Microb Pathog. https://doi.org/10.1016/j.micpath.2023.106161
Schmidt A, Frensch M, Schleheck D, Schink B, Muller N (2014) Degradation of acetaldehyde and its precursors by Pelobacter carbinolicus and P. acetylenicus. PLoS One 9(12):e115902. https://doi.org/10.1371/journal.pone.0115902
Seitz HK, Neuman MG (2021) The history of alcoholic liver disease: from an unrecognized disease to one of the most frequent diseases in hepatology. J Clin Med. https://doi.org/10.3390/jcm10040858
Sobh A, Loguinov A, Stornetta A, Balbo S, Tagmount A, Zhang L et al (2019) Genome-wide CRISPR screening identifies the tumor suppressor candidate OVCA2 as a determinant of tolerance to acetaldehyde. Toxicol Sci 169(1):235–245. https://doi.org/10.1093/toxsci/kfz037
Tacconi EM, Lai X, Folio C, Porru M, Zonderland G, Badie S et al (2017) BRCA1 and BRCA2 tumor suppressors protect against endogenous acetaldehyde toxicity. EMBO Mol Med 9(10):1398–1414. https://doi.org/10.15252/emmm.201607446
Wang H, Wang B, Li J, Zhu T (2019) Adsorption equilibrium and thermodynamics of acetaldehyde/acetone on activated carbon. Sep Purif Technol 209:535–541. https://doi.org/10.1016/j.seppur.2018.07.076
Woodley JM (2006) Microbial biocatalytic processes and their development. Adv Appl Microbiol 60:1–15. https://doi.org/10.1016/s0065-2164(06)60001-4
Yin C, Chen L, Qiu H, Xiong W, Lin S, Deng Z et al (2020) Production of high-value drug precursors by the whole-cell catalyst based on the transformation of ring-hydroxylating dioxygenase to aromatic compounds. Bioresource Technol Rep. https://doi.org/10.1016/j.biteb.2020.100521
Zenki KC, Mussulini BH, Rico EP, de Oliveira DL, Rosemberg DB (2014) Effects of ethanol and acetaldehyde in zebrafish brain structures: an in vitro approach on glutamate uptake and on toxicity-related parameters. Toxicol Vitro 28(5):822–828. https://doi.org/10.1016/j.tiv.2014.03.008
Zhang H, Fu L (2021) The role of ALDH2 in tumorigenesis and tumor progression: targeting ALDH2 as a potential cancer treatment. Acta Pharm Sin B 11(6):1400–1411. https://doi.org/10.1016/j.apsb.2021.02.008
Zhang Y, Li Z, Lu Y, Zhang J, Sun Y, Zhou J et al (2022) Characterization of Bacillus velezensis E2 with abilities to degrade ochratoxin A and biocontrol against aspergillus westerdijkiae fc-1. Toxicon 216:125–131. https://doi.org/10.1016/j.toxicon.2022.07.006
Zhang L, Wang B, Li K, Wang Z, Xu D, Su Y et al (2023a) Non-negligible health risks caused by inhalation exposure to aldehydes and ketones during food waste treatments in megacity Shanghai. Environ Pollut. https://doi.org/10.1016/j.envpol.2023.121448
Zhang T, Wei S, Liu Y, Cheng C, Ma J, Yue L et al (2023b) Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil. Front Microbiol. https://doi.org/10.3389/fmicb.2023.1167293
Zhu Y, Li Y, Xu Y, Zhang J, Ma L, Qi Q et al (2021) Development of bifunctional biosensors for sensing and dynamic control of glycolysis flux in metabolic engineering. Metab Eng 68:142–151
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This study was funded by the National Key R&D Program of China (Grant No. 2022YFE0118800).
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Conceptualization: HY; Data Curation: JW, ZW and MS; Investigation: JW, ZW and CL; Methodology: YL; Project administration: HY and QX; Resources: HY; Supervision: HY and QX; Validation: JW, ZW and CL; Visualization: JW and ZW; Writing—Original Draft: JW and ZW; Writing—Review & Editing: HY. All authors have read and agreed to the published version of the manuscript.
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Wang, J., Wang, Z., Liu, C. et al. Genome analysis of a newly isolated Bacillus velezensis-YW01 for biodegrading acetaldehyde. Biodegradation (2024). https://doi.org/10.1007/s10532-024-10075-4
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DOI: https://doi.org/10.1007/s10532-024-10075-4